Study of key operational parameters for the side-chain cleavage of sitosterol by free mycobacterial cells in bis-(2-ethylhexyl) phthalate

Biocatalysis and Biotransformation

Volumn 22, Issue 3, 2004, Pages 189-194

  Cruz, A ^a   Angelova, B ^a,b   Fernandes, P ^a,c   Cabral, J M S ^a   Pinheiro, Helena M ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b INSTITUTE OF MICROBIOLOGY   (Bulgaria)

^c UNIVERSIDADE LUSÓFONA DE HUMANIDADES E TECNOLOGIAS   (Portugal)

Author keywords

Androstenedione; Biotransformation in organic medium; Mycobacterium sp.; Phthalate; Sitosterol side chain cleavage

Indexed keywords

BIOCATALYSTS; CELLS; DRUG PRODUCTS PLANTS; SUBSTRATES;

ANDROSTENEDIONE (AD); SITOSTEROL;

BIOMATERIALS;

ANDROSTENEDIONE; CHOLESTEROL MONOOXYGENASE (SIDE CHAIN CLEAVING); ORGANIC SOLVENT; PHTHALIC ACID BIS(2 ETHYLHEXYL) ESTER; SITOSTEROL;

AERATION; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BIOCATALYST; CELL SUSPENSION; CHEMICAL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME KINETICS; ENZYME SUBSTRATE; MYCOBACTERIUM; NONHUMAN; QUANTUM YIELD; REACTOR MONITORING; STIRRED REACTOR; STRUCTURE ANALYSIS;

MYCOBACTERIUM; MYCOBACTERIUM SP.;

EID: 4844229254     PISSN: 10242422     EISSN: None     Source Type: Journal    
DOI: 10.1080/10242420412331283297     Document Type: Article

References (16)

1. Cruz, A., Fernandes, P., Cabral, J.M.S. and Pinheiro, H.M. (2001) Whole-cell bioconversion of β-sitosterol in aqueous-organic two-phase systems, J. Mol. Catalysis B: Enzymatic 11, 579-585.
2. Cruz, A., Fernandes, P., Cabral, J.M.S. and Pinheiro, H.M. (2002) Effect of phase composition on the whole-cell bioconversion of sitosterol in biphasic media, J. Mol. Catalysis B: Enzymatic 19-20, 371-375.
3. Daugulis, A.J. (2001) Two-phase partitioning bioreactors: a new technology platform for destroying xenobiotics, Tibtech 19, 457-462.
4. de Carvalho, C.C.C.R., Cruz, A., Angelova, B., Fernandes, P., Pons, M.N., Pinheiro, H.M., Cabral, J.M.S. and da Fonseca, M.M.R. (2004) Behaviour of Mycobacterium sp. NRRL B-3805 in aqueous, organic-aqueous and organic media studied by fluorescence microscopy, Appl. Microbiol. Biotechnol. 64(5), 695-701.
5. Dias, A.C.P., Cabral, J.M.S. and Pinheiro, H.M. (1994) Sterol sidechain cleavage with immobilized Mycobacterium cells in water-immiscible organic solvents, Enzyme Microb. Technol. 16, 708-714.
6. Fernandes, P., Cabral, J.M.S. and Pinheiro, H.M. (1998) Influence of some operational parameters on the bioconversion of sitosterol with immobilized whole cells in organic medium, J. Mol. Catalysis B: Enzymatic 5, 307-310.
7. Fernandes, P., Cruz, A., Angelova, B., Pinheiro, H.M. and Cabral, J.M.S. (2003) Microbial conversion of steroid compounds: recent developments, Enzyme Microb. Technol 32, 688-705.
8. León, R., Fernandes, P., Pinheiro, H.M. and Cabral, J.M.S. (1998) Whole cell biocatalysis in organic media, Enzyme Microb. Technol. 23, 483-500.
9. Lo, C.-K., Pan, C.-P. and Liu, W.-H. (2002) Production of testosterone from phytosterol using a single-step microbial transformation by a mutant of Mycobacterium sp., J. Ind. Microbio. Biotechnol. 28, 280-283.
10. Mahato, S.B. and Garai, S. (1997) Advances in microbial steroid biotransformation, Steroids 62, 332-345.
11. Mutafov, S., Angelova, B., Avramova, T., Boyadjieva, L. and Dimova, I. (1997) The inducibility of 9α-steroid hydroxylating activity in resting Rhodococcus sp. cells, Process Biochem. 32, 585-589.
12. Schmid, A., Dordick, J.S., Hauer, B., Kiener, A., Wubbolts, M. and Witholt, B. (2001) Industrial biocatalysis today and tomorrow, Nature 409, 258-268.
13. Staebler, A., Cruz, A., van der Goot, W., Pinheiro, H.M., Cabral, J.M.S., and Fernandes P. (2004) Optimization of androstenedione production in an organic-aqueous two-liquid phase system, J. Mol. Catalysis B: Enzymatic 29, 19-23.
14. Stark, D. and von Stockar, U. (2003) In Situ Product Removal (ISPR) in whole cell biotechnology during the last twenty years, Advances Biochem. Eng. Biotechnol. 80, 115-148.
15. Szentirmai, A. (1990) Microbial physiology of sidechain degradation of sterols, J. Ind. Microbiol. 6, 101-116.
16. Vermuë, M., Sikkema, J., Verheul, A., Bakker, R. and Tramper, J. (1993) Toxicity of homologous series of organic solvents for the gram-positive bacteria Arthrobacter and Nocardia sp. and the gram-negative bacteria Acinetobacter and Pseudomonas sp., Biotechnol. Bioeng. 42, 747-758.