The AdLMP-1 transfection in two different cells; AF cells, chondrocytes as potential cell therapy candidates for disc degeneration

Acta Neurochirurgica

Volumn 150, Issue 8, 2008, Pages 803-810

  Kuh, S U ^a   Zhu, Y ^b   Li, J ^b   Tsai, K J ^b   Fei, Q ^b   Hutton, W C ^b   Yoon, S T ^a,c  

^a Yonsei University College of Medicine   (South Korea)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Emory Spine Center   (United States)

Author keywords

AdLMP 1; Anulus fibrosus cell; Chondrocyte; Proteoglycan

Indexed keywords

AGGRECAN; BONE MORPHOGENETIC PROTEIN 2; COLLAGEN TYPE 1; COLLAGEN TYPE 2; GLYCOSAMINOGLYCAN POLYSULFATE; GREEN FLUORESCENT PROTEIN; LIM MINERALIZATION PROTEIN; MESSENGER RNA; METHYLENE BLUE; OSTEOGENIC PROTEIN 1; PROTEIN;

ANIMAL CELL; ANULUS FIBROSUS CELL; ARTICLE; CARTILAGE CELL; CELL; CELL CULTURE; CELL THERAPY; CONTROLLED STUDY; CULTURE MEDIUM; GENETIC TRANSFECTION; IMMUNOFLUORESCENCE; INTERVERTEBRAL DISK DEGENERATION; LUMBAR DISK; MONOLAYER CULTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; STAINING; UPREGULATION;

AGGRECANS; BONE MORPHOGENETIC PROTEINS; CELL TRANSPLANTATION; CHONDROCYTES; COLLAGEN TYPE I; COLLAGEN TYPE II; GENE EXPRESSION REGULATION; GENE THERAPY; GLYCOSAMINOGLYCANS; HUMANS; INTERVERTEBRAL DISK; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPINAL DISEASES; TRANSFECTION; TRANSFORMING GROWTH FACTOR BETA;

EID: 48349134844     PISSN: 00016268     EISSN: 09420940     Source Type: Journal    
DOI: 10.1007/s00701-008-1617-7     Document Type: Article

References (17)

1. J Antoniou T Steffen F Nelson N Winterbottom AP Hollander RA Poole M Aebi M Alini 1996 The human lumbar intervertebral disc: evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration J Clin Invest 98 996 1003
2. B Barnes SD Boden J Louis-Ugbo PR Tomak JS Park MS Park A Minamide 2005 Lower dose of rhBMP-2 achieves spine fusion when combined with an osteoconductive bulking agent in non-human primates Spine 30 1127 1133
3. SD Boden 2000 Biology of lumbar spine fusion and use of bone graft substitutes: present, future, and next generation Tissue Eng 6 383 399
4. SD Boden J Kang H Sandhu JG Heller 2002 Use of recombinant human bone morphogenetic protein-2 to achieve posterolateral lumbar spine fusion in humans: a prospective, randomized clinical pilot trial: 2002 Volvo Award in clinicalstudies Spine 27 2662 2673
5. SD Boden L Titus G Hair Y Liu M Viggeswarapu MS Nanes C Baranowski 1998 Lumbar spine fusion by local gene therapy with a cDNA encoding a novel osteoinductive protein (LMP-1) Spine 23 2486 2492
6. SL Cheng J Lou NM Wright CF Lai LV Avioli KD Riew 2001 In vitro and in vivo induction of bone formation using a recombinant adenoviral vector carrying the human BMP-2 gene Calcif Tissue Int 68 87 94
7. T Kai G Shao-qing D Geng-ting 2003 In vivo evaluation of bone marrow stromal-derived osteoblastsporous calcium phosphate ceramic composites as bone graft substitute for lumbar intervertebral spinal fusion Spine 28 1653 1658
8. DJ Kim SH Moon H Kim UH Kwon MS Park KJ Han 2003 Bone morphogenetic protein-2 facilitates expression of chondrogenic, not osteogenic, phenotype of human intervertebral disc cells Spine 28 2679 2684
9. KS Kim ST Yoon JS Park J Li MS Park WC Hutton 2003 Inhibition of proteoglycan and type II collagen synthesis of disc nucleus cells by nicotine J Neurosurg 99 291 297
10. J Li ST Yoon WC Hutton 2004 Effect of bone morphogenetic protein-2 (BMP-2) on matrix production, other BMPs, and BMP receptors in rat intervertebral disc cells J Spinal Disord Tech 17 423 428
11. JS Park K Nagata 2004 BMP and LMP-1 for intervertebral disc regeneration Clin Calcium 14 76 78
12. E Pola W Gao Y Zhou R Pola W Lattanzi C Sfeir A Gambotto PD Robbins 2004 Efficient bone formation by gene transfer of human LIM mineralization protein-3 Gene Ther 11 683 693
13. PJ Roughley M Alini J Antoniou 2002 The role of proteoglycans in aging, degeneration and repair of the intervertebral disc Biochem Soc Trans 30 869 874
14. HS Sandhu 2003 Bone morphogenetic proteins and spinal surgery Spine 28 S64 S73
15. ST Yoon KS Kim J Li JS Park T Akamaru WA Elmer WC Hutton 2003 The effect of bone morphogenetic protein-2 on rat intervertebral disc cells in vitro Spine 28 1773 1780
16. M Viggeswarapu SD Boden Y Liu GA Hair J Louis-Ugbo H Murakami HS Kim MT Mayr WC Hutton L Titus 2001 Adenoviral delivery of LIM mineralization protein-1 induces new-bone formation in vitro and in vivo J Bone Joint Surg Am 83-A 364 376
17. ST Yoon JS Park KS Kim J Li ES Attallah-Wasif WC Hutton SD Boden 2004 ISSLS prize winner: LMP-1 upregulates intervertebral disc cell production of proteoglycans and BMPs in vitro and in vivo Spine 29 2603 2611