Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Witusik, Monika ^a   Piaskowski, Sylwester ^a   Hulas Bigoszewska, Krystyna ^a   Zakrzewska, Magdalena ^a   Gresner, Sylwia M ^a   Azizi, S Ausim ^b   Krynska, Barbara ^b   Liberski, Pawel P ^a   Rieske, Piotr ^a  

^a MEDICAL UNIVERSITY OF LODZ   (Poland)

^b TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL CULTURE; BIOACTIVITY; CELLS; DIFFERENTIATION (CALCULUS); PEPTIDES; TURBULENT FLOW;

CULTURE CONDITIONS; CULTURES (TRADITIONAL); ENVIRONMENTAL CHANGES; ENVIRONMENTAL CONDITIONING; EXTRACELLULAR; FIBRONECTIN (FN); GLIAL CELLS; GROWTH FACTORS; IN-CELL; IN-VITRO; INTRINSIC FACTORS; NEURAL CELLS; NEURAL STEM CELLS; NEURONAL CELLS; SERUM FREE MEDIUM; TESTING CONDITIONS; VIMENTIN;

CELL CULTURE;

BASIC FIBROBLAST GROWTH FACTOR; FIBRONECTIN; GLIAL CELL LINE DERIVED NEUROTROPHIC FACTOR; GLIAL FIBRILLARY ACIDIC PROTEIN; HERMES ANTIGEN; MICROTUBULE ASSOCIATED PROTEIN 2; PROTEIN S100B; SONIC HEDGEHOG PROTEIN; TRANSCRIPTION FACTOR SOX2; VIMENTIN; BIOLOGICAL MARKER; CD44 PROTEIN, HUMAN; DNA BINDING PROTEIN; FIBROBLAST GROWTH FACTOR; HIGH MOBILITY GROUP B PROTEIN; MAP2 PROTEIN, HUMAN; MICROTUBULE ASSOCIATED PROTEIN; NERVE GROWTH FACTOR; NERVE PROTEIN; PROTEIN S 100; S 100 CALCIUM BINDING PROTEIN BETA SUBUNIT; S-100 CALCIUM-BINDING PROTEIN BETA SUBUNIT; SOX-2 TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; ASTROCYTE; CATECHOLAMINE NERVE CELL; CELL CULTURE; CELL CULTURE MONITORING; CELL DIFFERENTIATION; CELL POPULATION; CLINICAL PROTOCOL; CONTROLLED STUDY; CULTURE MEDIUM; FETUS; GABAERGIC SYSTEM; GLIA CELL; HUMAN; HUMAN CELL; NEURAL STEM CELL; NONHUMAN; CELL LINEAGE; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; GLIA; METABOLISM; METHODOLOGY; NERVE CELL; PHYSIOLOGY; STEM CELL;

ANTIGENS, CD44; ASTROCYTES; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; CELLS, CULTURED; CULTURE MEDIA, SERUM-FREE; DNA-BINDING PROTEINS; FIBROBLAST GROWTH FACTORS; FIBRONECTINS; GLIAL FIBRILLARY ACIDIC PROTEIN; HMGB PROTEINS; HUMANS; MICROTUBULE-ASSOCIATED PROTEINS; NERVE GROWTH FACTORS; NERVE TISSUE PROTEINS; NEUROGLIA; NEURONS; S100 PROTEINS; STEM CELLS; TRANSCRIPTION FACTORS; VIMENTIN;

EID: 48249146508     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-56     Document Type: Article

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