Purification of monoclonal antibody from tobacco extract using membrane-based bioseparation techniques

Journal of Membrane Science

Volumn 323, Issue 1, 2008, Pages 159-166

  Yu, Deqiang ^a   McLean, Michael D ^b   Hall, J Christopher ^b   Ghosh, Raja ^a  

^a MCMASTER UNIVERSITY   (Canada)

^b UNIVERSITY OF GUELPH   (Canada)

Author keywords

Bioseparation; Membrane chromatography; Monoclonal antibody; Transgenic tobacco; Ultrafiltration

Indexed keywords

AGRICULTURAL PRODUCTS; ALLERGIES; BIOMOLECULES; CHEMICALS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; DESALINATION; GRADIENT METHODS; HYDROPHOBIC CHROMATOGRAPHY; HYDROPHOBICITY; ION CHROMATOGRAPHY; ION EXCHANGE; ION EXCHANGE MEMBRANES; LIGANDS; MEMBRANES; MERGERS AND ACQUISITIONS; MONOCLONAL ANTIBODIES; PLANTS (BOTANY); PROTEINS; PURIFICATION; SURFACE PLASMON RESONANCE; TOBACCO; ULTRAFILTRATION;

AFFINITY LIGANDS; ANTIBODY CAPTURE; ANTIBODY PURIFICATION; BIO-SEPARATION; BOUND PROTEINS; ELSEVIER (CO); GRADIENT ELUTION; HIGH RESOLUTIONS; HIGH-PURITY; HYDROPHOBIC INTERACTIONS; IMMUNOTOXICITY; LARGE SCALE PRODUCTIONS; MEMBRANE CHROMATOGRAPHY; MEMBRANE-BASED PROCESS; MOBILE PHASE; MONOCLONAL ANTIBODIES (MOABS); PROTEIN A; PURIFICATION METHODS; SINGLE PEAK; SINGLE STEP; SIZE EXCLUSION; TRANSGENIC PLANTS;

ANTIBODIES;

MONOCLONAL ANTIBODY; PROTEIN A;

ANIMAL CELL; ARTICLE; BIOPOLISHING; BIOSEPARATION; CATION EXCHANGE; CHROMATOGRAPHY; CONTROLLED STUDY; GEL PERMEATION CHROMATOGRAPHY; IMMUNOTOXICITY; ION EXCHANGE CHROMATOGRAPHY; MEMBRANE FILTER; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN PURIFICATION; TOBACCO; ULTRAFILTRATION;

EID: 48149101101     PISSN: 03767388     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.memsci.2008.06.019     Document Type: Article

References (28)

1. Schillberga S., Twymanb R.M., and Fischera R. Opportunities for recombinant antigen and antibody expression in transgenic plants-technology assessment. Vaccine 23 (2005) 1764
2. Ma J.K., Drake P.M., Chargelegue D., Obregon P., and Prada A. Antibody processing and engineering in plants, and new strategies for vaccine production. Vaccine 23 (2005) 1814
3. Ko K., and Koprowski H. Plant biopharming of monoclonal antibodies. Virus Res. 111 (2005) 93
4. Hiatt A., Cafferkey R., and Bowdish K. Production of antibodies in transgenic plants. Nature 342 (1989) 76
5. Ma J.K., Hiatt A., Hein M., Vine N.D., Wang F., Stabila P., Van Dolleweerd C., Mostov K., and Lehner T. Generation and assembly of secretory antibodies in plants. Science 268 (1995) 716
6. McLean M.D., Almquist K.C., Niu Y., Kimmel R., Lai Z., Schreiber J.R., and Hall J.C. A human anti-pseudomonas aeruginosa O6ad IgG1 expressed in transgenic tobacco is capable of recruiting immune system effector function in vitro. Antimicrob. Agents Chemother. 51 (2007) 3322
7. Olea-Popelka F.C., McLean M.D., Horsman J., Almquist K., Brandle J.E., and Hall J.C. Increasing expression of an anti-picloram single-chain variable fragment (ScFv) antibody and resistance to picloram in transgenic tobacco (Nicotiana tabacum). J. Agric. Food Chem. 53 (2005) 6683
8. Almquist K.C., McLean M.D., Niu Y., Byrne G., Olea-Popelka F.C., Murrant C., Barclay J., and Hall J.C. Expression of an anti-botulinum toxin A neutralizing single-chain Fv recombinant antibody in transgenic tobacco. Vaccine 24 (2006) 2079
9. Daniell H., Streatfield S.J., and Wycoff K. Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci. 6 (2001) 219
10. Farid S.S. Established bioprocesses for producing antibodies as a basis for future planning. Adv. Biochem. Eng. Biotechnol. 101 (2006) 1
11. Stoger E., Sack M., Nicholson L., Fischer R., and Christou P. Recent progress in plantibody technology. Curr. Pharm. Des. 11 (2005) 2439
12. Schillberg S., Fischera R., and Emans N. 'Molecular farming' of antibodies in plants. Naturwissenschaften 90 (2003) 145
13. Carlson K.L. Flexibility-the guiding principle for antibody manufacturing. Nat. Biotechnol. 23 (2005) 1054
14. Arakawa T., Philo J.S., Tsumoto K., Yumioka R., and Ejima D. Elution of antibodies from a protein-A column by aqueous arginine solutions. Protein Expr. Purif. 36 (2004) 244
15. Ghosh R. Protein separation using membrane chromatography: opportunities and challenges. J. Chromatogr. A 952 (2002) 13
16. Zou H., Luo Q., and Zhou D. Affinity membrane chromatography for the analysis and purification of proteins. J. Biochem. Biophys. Methods 49 (2001) 199
17. Castilho L.R., Anspach F.B., and Deckwer W.D. Comparison of affinity membranes for the purification of immunoglobulins. J. Membr. Sci. 207 (2002) 253
18. Knudsen H.L., Fahrner R.L., Xu Y., Norling L.A., and Blank G.S. Membrane ion-exchange chromatography for process-scale antibody purification. J. Chromatogr. A 907 (2001) 145
19. Phillips M., Cormier J., Ferrence J., Dowd C., Kiss R., Lutz H., and Carter J. Performance of a membrane adsorber for trace impurity removal in biotechnology manufacturing. J. Chromatogr. A 1078 (2004) 74
20. Ghosh R. Separation of proteins using hydrophobic interaction membrane chromatography. J. Chromatogr. A 923 (2001) 59
21. Ghosh R. Fractionation of human plasma proteins by hydrophobic interaction membrane chromatography. J. Membr. Sci. 260 (2005) 112
22. Ghosh R., and Wang L. Purification of humanized monoclonal antibody by hydrophobic interaction membrane chromatography. J. Chromatogr. A 1107 (2006) 104
23. Yu D., Chen X., Pelton R., and Ghosh R. Paper-PEG based membranes for hydrophobic interaction chromatography: Purification of monoclonal antibody. Biotechnol. Bioeng. 99 (2007) 1434
24. Yu D., McLean M.D., Hall J.C., and Ghosh R. Purification of monoclonal antibody from transgenic tobacco plant using membrane chromatographic processes. J. Chromatogr. A 1187 (2008) 128
25. Ghosh R., and Wong T. Effect of module design on the efficiency of membrane chromatographic separation processes. J. Membr. Sci. 281 (2006) 532
26. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680
27. Balasubramaniam D., Wilkinson C., Cott K.V., and Zhang C. Tobacco protein separation by aqueous two-phase extraction. J. Chromatogr. A 989 (2003) 119
28. Hester J.F., and Mayes A.M. Design and performance of foul-resistant poly(vinylidene fluoride) membranes prepared in a single-step by surface segregation. J. Membr. Sci. 202 (2002) 119