A small subset of signal peptidase residues are perturbed by signal peptide binding

Chemical Biology and Drug Design

Volumn 72, Issue 2, 2008, Pages 140-146

  Musial Siwek, Monika ^a,b   Yeagle, Philip L ^a,c   Kendall, Debra A ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

^b YALE UNIVERSITY   (United States)

^c RUTGERS UNIVERSITY   (United States)

Author keywords

Membrane protein; NMR; Signal peptidase; Signal peptide; Substrate binding

Indexed keywords

ALKALINE PHOSPHATASE; ASPARTIC ACID; ESCHERICHIA COLI PROTEIN; GLUTAMIC ACID; GLUTAMINE; GLYCINE; ISOLEUCINE; LYSINE; MEMBRANE PROTEIN; METHIONINE; PROTEIN PRECURSOR; SERINE; SIGNAL PEPTIDASE; SIGNAL PEPTIDASE I; SIGNAL PEPTIDE; THREONINE; VALINE;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; ENZYME SUBUNIT; ESCHERICHIA COLI; HETERONUCLEAR SINGLE QUANTUM COHERENCE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; PROTON NUCLEAR MAGNETIC RESONANCE;

ESCHERICHIA COLI; MEMBRANE PROTEINS; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, TERTIARY; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

EID: 47949106667     PISSN: 17470277     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1747-0285.2008.00685.x     Document Type: Article

References (20)

1. Wolfe P.B., Wickner W., Goodman J.M. (1983) Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope. J Biol Chem 258 : 12073 12080.
2. Whitley P., Nilsson L., von Heijne G. (1993) Three-dimensional model for the membrane domain of Escherichia coli leader peptidase based on disulfide mapping. Biochemistry 32 : 8534 8539.
3. Paetzel M., Dalbey R.E., Strynadka N.C. (2002) Crystal structure of a bacterial signal peptidase apoenzyme: implications for signal peptide binding and the Ser-Lys dyad mechanism. J Biol Chem 277 : 9512 9519.
4. Paetzel M., Dalbey R.E., Strynadka N.C. (1998) Crystal structure of a bacterial signal peptidase in complex with a beta-lactam inhibitor. Nature 396 : 186 190.
5. Ekici O.D., Karla A., Paetzel M., Lively M.O., Pei D., Dalbey R.E. (2007) Altered -3 substrate specificity of Escherichia coli signal peptidase 1 mutants as revealed by screening a combinatorial peptide library. J Biol Chem 282 : 417 425.
6. Wang L., Miller A., Kendall D.A. (2000) Signal peptide determinants of SecA binding and stimulation of ATPase activity. J Biol Chem 275 : 10154 10159.
7. Izard J.W., Doughty M.B., Kendall D.A. (1995) Physical and conformational properties of synthetic idealized signal sequences parallel their biological function. Biochemistry 34 : 9904 9912.
8. Paetzel M., Chernaia M., Strynadka N., Tschantz W., Cao G., Dalbey R.E., James M.N.R. (1995) Crystallization of a soluble, catalytically active form of Escherichia coli leader peptidase. Proteins 23 : 122 125.
9. Delaglio F., Grzesiek S., Vuister G.W., Zhu G., Pfeifer J., Bax A. (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6 : 277 293.
10. Deber C.M., Wang C., Liu L.P., Prior A.S., Agrawal S., Muskat B.L., Cuticchia A.J. (2001) TM Finder: a prediction program for transmembrane protein segments using a combination of hydrophobicity and nonpolar phase helicity scales. Protein Sci 10 : 212 219.
11. Liu L.P., Deber C.M. (1998) Guidelines for membrane protein engineering derived from de novo designed model peptides. Biopolymers 47 : 41 62.
12. Melnyk R.A., Partridge A.W., Yip J., Wu Y., Goto N.K., Deber C.M. (2003) Polar residue tagging of transmembrane peptides. Biopolymers 71 : 675 685.
13. Tang Y.C., Deber C.M. (2004) Aqueous solubility and membrane interactions of hydrophobic peptides with peptoid tags. Biopolymers 76 : 110 118.
14. Tschantz W.R., Paetzel M., Cao G., Suciu D., Inouye M., Dalbey R.E. (1995) Characterization of a soluble, catalytically active form of Escherichia coli leader peptidase: requirement of detergent or phospholipid for optimal activity. Biochemistry 34 : 3935 3941.
15. Hajduk P.J., Meadows R.P., Fesik S.W. (1997) Discovering high-affinity ligands for proteins. Science 278 : 497 499.
16. Shuker S.B., Hajduk P.J., Meadows R.P., Fesik S.W. (1996) Discovering high-affinity ligands for proteins: SAR by NMR. Science 274 : 1531 1534.
17. Tschantz W.R., Sung M., Delgado-Partin V.M., Dalbey R.E. (1993) A serine and a lysine residue implicated in the catalytic mechanism of the Escherichia coli leader peptidase. J Biol Chem 268 : 27349 27354.
18. Carlos J.L., Klenotic P.A., Paetzel M., Strynadka N.C., Dalbey R.E. (2000) Mutational evidence of transition state stabilization by serine 88 in Escherichia coli type I signal peptidase. Biochemistry 39 : 7276 7283.
19. Karla A., Lively M.O., Paetzel M., Dalbey R. (2005) The identification of residues that control signal peptidase cleavage fidelity and substrate specificity. J Biol Chem 280 : 6731 6741.
20. Yeagle P.L., Sen A. (1986) Hydration and the lamellar to hexagonal II phase transition of phosphatidylethanolamine. Biochemistry 25 : 7518 7522.