Isolated spermatogonia protrude active pseudopodia in vitro

Fertility and Sterility

Volumn 90, Issue 2, 2008, Pages 453-455

  Tanaka, Atsushi ^a   Nagayoshi, Motoi ^a   Awata, Shoichiro ^a   Himeno, Norio ^a   Tanaka, Izumi ^a   Kusunoki, Hiroshi ^b  

^a Saint Mother Obstetrics and Gynecology Clinic   (Japan)

^b KOBE UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; HISTONE H2AX;

ANIMAL TISSUE; ARTICLE; CLINICAL ARTICLE; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMMUNOCOMPETENT CELL; IN VITRO STUDY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PSEUDOPODIUM; SPERMATOCYTE; SPERMATOGONIUM; TESTIS;

ADULT; ANIMALS; AZOOSPERMIA; HUMANS; MALE; MICE; PSEUDOPODIA; SPERMATOGONIA;

EID: 47849110866     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fertnstert.2007.06.055     Document Type: Article

References (21)

1. Witschi E. Migration of the germ cells of human embryos from the yolk sac to the primitive gonadal folds. Carnegie Contr Embryol 32 (1948) 67-80
2. Chiquoine A.D. The identification, origin, and migration of the primordial germ cells in the mouse embryo. Anat Rec 118 (1954) 135-146
3. Ulvik N.M. Stage-dependent topographical relationship of spermatogonia and early spermatocytes to Sertoli-Sertoli interspaces in the rat testis. Int J Androl 6 (1983) 469-481
4. Ulvik N.M. Selective uptake by the Sertoli cells of cytoplasm from normal spermatogonia in the rat testis. Int J Androl 6 (1983) 367-374
5. Tesarik J., Mendoza C., and Testart J. Viable embryos from injection of round spermatids into oocytes. N Engl J Med 333 (1995) 525
6. Hannay T. New Japanese IVF method finally made available in Japan. Nature Med 1 (1995) 289-290
7. Cremates N., Bernabeu R., Barros A., and Sousa M. In-vitro maturation of round spermatids using co-culture on Vero cells. Hum Reprod 14 (1999) 1287-1293
8. Cremates N., Sousa M., Bernabeu R., and Barros A. Developmental potential of elongating and elongated spermatids obtained after in-vitro maturation of isolated round spermatids. Hum Reprod 16 (2001) 1938-1944
9. Tesarik J., Balaban B., Isikal A., Alatas C., Urman B., Aksoy S., et al. In-vitro spermatogenesis resumption in men with maturation arrest: relationship with in-vivo blocking stage and serum FSH. Hum Reprod 15 (2000) 1350-1354
10. Tesarik J., Guido M., Mendoza C., and Gerco E. Human spermatogenesis in vitro: respective effects of follicle-stimulating hormone and testosterone on meiosis, spermiogenesis, and Sertoli cell apoptosis. J Clinic Endocrinol Metab 33 (1998) 4467-4473
11. Tesarik J., Gerco E., Rienzi L., Ubaldi F., Guido M., Cohen-Bacrie P., et al. Differentiation of spermatogenic cells during in-vitro culture of testicular biopsy samples from patients with obstructive azoospermia: effect of recombinant follicle stimulating hormone. Hum Reprod 13 (1998) 2772-2781
12. Tesarik J., Bahceci M., Ozcan C., Greco E., and Mendoza C. Restoration of fertility by in vitro spermatogenesis. Lancet 353 (1999) 555
13. Tanaka A., Nagayoshi M., Awata S., Mawatari Y., Tanaka I., and Kusunoki H. Completion of meiosis in human primary spermatocytes through in vitro coculture with Vero cells. Fertil Steril 79 (2003) 795-801
14. Vanderzwalmen P., Nijs M., Stecher A., Zech H., Bertin G., Lejeune B., et al. Is there a future for spermatid injection?. Hum Reprod 13 (1998) 71-84
15. Vanderzwalmen P., Nijs M., Schoysman R., Bertin G., Lejeune B., Vandamme B., et al. The problems of spermatid microinjection in the human: the need for accurate morphological approach and selective methods for viable and normal cells. Hum Reprod 13 (1998) 514-519
16. Marh J., Tres L.L., Yamazaki Y., Yanagimachi R., and Kierszenbaum A.L. Mouse round spermatids developed in vitro from preexisting spermatocytes can produce normal offspring by nuclear injection into in vivo-developed mature oocytes. Biol Reprod 69 (2003) 169-176
17. Johnson L., Neaves W.B., Barnard J.J., Keillor G.E., Brown S.W., and Yanagimachi R. A comparative morphological study of human germ cells in vitro or in situ within seminiferous tubules. Biol Reprod 61 (1999) 927-934
18. Johnson L., Staub C., Neaves W.B., and Yanagimachi R. Live human germ cells in the context of their spermatogenic stages. Hum Reprod 16 (2001) 1575-1582
19. Lacham-Kaplan O. In vivo and in vitro differentiation of male germ cells in the mouse. Reproduction 128 (2004) 147-152
20. Hamer G., Roepers-Gajadien H.L., van Duyn-Goedhart A., Gademan I.S., Kal H.B., van Buul P.P., et al. DNA double-strand breaks and γ-H2AX signaling in the testis. Biol Reprod 68 (2003) 628-634
21. Brinster R.L., and Avarbock M.R. Germline transmission of donor haplotype following spermatogonial transplantation. Proc Natl Acad Sci USA 91 (1994) 11303-11307