Detection of conformational changes in immunoglobulin G using isothermal titration calorimetry with low-molecular-weight probes

Analytical Biochemistry

Volumn 380, Issue 2, 2008, Pages 303-309

  Rispens, Theo ^a,b   Lakemond, Catriona M M ^c   Derksen, Ninotska I L ^a,b   Aalberse, Rob C ^a,b  

^a SANQUIN RESEARCH   (Netherlands)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

^c WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

8 Anilino 1 naphthalene sulfonate; Fluorescence; Heat induced denaturation; Immunoglobulin; Isothermal titration calorimetry

Indexed keywords

BINDING SITES; CALORIMETERS; CALORIMETRY; DISSOCIATION; ENTHALPY; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; ISOTHERMS; MOLAR RATIO; MOLECULAR WEIGHT; PROTEINS; TITRATION;

8-ANILINO-1-NAPHTHALENE SULFONATE; CONFORMATIONAL CHANGE; DISSOCIATION CONSTANT; FLUORESCENCE MEASUREMENTS; IMMUNOGLOBULIN; ISOTHERMAL TITRATION CALORIMETRY; LOW MOLECULAR WEIGHT; MONOMERIC PRECURSORS;

PROBES;

8 ANILINO 1 NAPHTHALENESULFONIC ACID; ADALIMUMAB; IMMUNOGLOBULIN G; OMALIZUMAB;

ANALYTIC METHOD; ARTICLE; BINDING SITE; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; HEAT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTHERMAL TITRATION CALORIMETRY; MOLECULAR WEIGHT; PRECIPITATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; SPECTROPHOTOMETER; THERMODYNAMICS;

EID: 47749085443     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.06.001     Document Type: Article

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