HPLC and mass spectrometric characterization of a candidate reference material for the alcohol biomarker carbohydrate-deficient transferrin (CDT)

Clinica Chimica Acta

Volumn 395, Issue 1-2, 2008, Pages 142-145

  Oberrauch, Walter ^a   Bergman, Ann Charlotte ^b   Helander, Anders ^b  

^a RAD Data Communications GmbH   (Germany)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Alcohol biomarker; Carbohydrate deficient transferrin (CDT); HPLC; Mass spectrometry; Reference material; Standardization

Indexed keywords

ALCOHOL; BIOLOGICAL MARKER; CARBOHYDRATE DEFICIENT TRANSFERRIN; TRANSFERRIN;

ARTICLE; CORRELATION ANALYSIS; ELECTROSPRAY MASS SPECTROMETRY; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MASS SPECTROMETRY; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN ANALYSIS; REFERENCE SUBSTANCE; REFERENCE VALUE; SAMPLING;

ALCOHOLISM; BIOLOGICAL MARKERS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ETHANOL; GLYCOSYLATION; HUMANS; N-ACETYLNEURAMINIC ACID; REFERENCE STANDARDS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TIME FACTORS; TRANSFERRIN;

EID: 47649095038     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2008.06.001     Document Type: Article

References (17)

1. de Jong G., van Dijk J.P., and van Eijk H.G. The biology of transferrin. Clin Chim Acta 190 (1990) 1-46
2. Helander A., Husa A., and Jeppsson J.O. Improved HPLC method for carbohydrate-deficient transferrin in serum. Clin Chem 49 (2003) 1881-1890
3. Kamboh M.I., and Ferrell R.E. Human transferrin polymorphism. Hum Hered 37 (1987) 65-81
4. Jaeken J., and Matthijs G. Congenital disorders of glycosylation: a rapidly expanding disease family. Annu Rev Genomics Hum Genet 8 (2007) 261-278
5. Stibler H. Carbohydrate-deficient transferrin in serum: a new marker of potentially harmful alcohol consumption reviewed. Clin Chem 37 (1991) 2029-2037
6. Bergström J.P., and Helander A. Influence of alcohol use, ethnicity, age, gender, BMI and smoking on the serum transferrin glycoform pattern: implications for use of carbohydrate-deficient transferrin (CDT) as alcohol biomarker. Clin Chim Acta 388 (2008) 59-67
7. Arndt T. Carbohydrate-deficient transferrin as a marker of chronic alcohol abuse: a critical review of preanalysis, analysis, and interpretation. Clin Chem 47 (2001) 13-27
8. Bortolotti F., De Paoli G., and Tagliaro F. Carbohydrate-deficient transferrin (CDT) as a marker of alcohol abuse: a critical review of the literature 2001-2005. J Chromatogr B Analyt Technol Biomed Life Sci 841 (2006) 96-109
9. Jeppsson J.O., Arndt T., Schellenberg F., et al. Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference method. Clin Chem Lab Med 45 (2007) 558-562
10. Frenoy N., Goussault Y., and Bourrillon R. Preparation de la transferrine du serum humain. [Preparation of human serum transferrin]. Clin Chim Acta 32 (1971) 243-249
11. Helander A., and Bergström J.P. Determination of carbohydrate-deficient transferrin in human serum using the Bio-Rad %CDT by HPLC test. Clin Chim Acta 371 (2006) 187-190
12. Bianchi V., Goy M.C., Pinca T., Sida G., Roveta A., and Arfini C. Application of immunosubtraction in the determination of carbohydrate-deficient transferrin [Abstract]. Biochim Clin 30 (2006) 369
13. del Castillo Busto M.E., Montes-Bayon M., Blanco-Gonzalez E., Meija J., and Sanz-Medel A. Strategies to study human serum transferrin isoforms using integrated liquid chromatography ICPMS, MALDI-TOF, and ESI-Q-TOF detection: application to chronic alcohol abuse. Anal Chem 77 (2005) 5615-5621
14. Kleinert P., Kuster T., Durka S., et al. Mass spectrometric analysis of human transferrin in different body fluids. Clin Chem Lab Med 41 (2003) 1580-1588
15. Landberg E., Påhlsson P., Lundblad A., Arnetorp A., and Jeppsson J.O. Carbohydrate composition of serum transferrin isoforms from patients with high alcohol consumption. Biochem Biophys Res Commun 210 (1995) 267-274
16. Peter J., Unverzagt C., Engel W.D., Renauer D., Seidel C., and Hösel W. Identification of carbohydrate deficient transferrin forms by MALDI-TOF mass spectrometry and lectin ELISA. Biochim Biophys Acta 1380 (1998) 93-101
17. Flahaut C., Michalski J.C., Danel T., Humbert M.H., and Klein A. The effects of ethanol on the glycosylation of human transferrin. Glycobiology 13 (2003) 191-198