Biophysical and ion channel functional characterization of the Torpedo californica nicotinic acetylcholine receptor in varying detergent-lipid environments

Journal of Membrane Biology

Volumn 223, Issue 1, 2008, Pages 13-26

  Asmar Rovira, Guillermo A ^a   Asseo García, Aloysha M ^b   Quesada, Orestes ^b   Hanson, Michael A ^a   Cheng, Anchi ^a   Nogueras, Carlos ^b   Lasalde Dominicci, José A ^b   Stevens, Raymond C ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF PUERTO RICO   (Puerto Rico)

Author keywords

Biophysical characterization; Detergent; Ion channel function; Lipid; nAChR; Planar lipid bilayer

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; DETERGENT; DICHLORODIFLUOROMETHANE; ION CHANNEL; NICOTINIC RECEPTOR; PYRANOSIDE;

ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; GAS CHROMATOGRAPHY; LIPID BILAYER; LIPID DEGRADATION; NONHUMAN; PROTEIN ANALYSIS; SCANNING ELECTRON MICROSCOPY; STATISTICAL SIGNIFICANCE; TORPEDO CALIFORNICA;

ANIMALS; BIOPHYSICAL PROCESSES; DETERGENTS; DIMERIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ION CHANNELS; LIPIDS; MICROSCOPY, ELECTRON; MODELS, CHEMICAL; MOLECULAR STRUCTURE; RECEPTORS, NICOTINIC; TORPEDO;

TORPEDINIFORMES; TORPEDO CALIFORNICA;

EID: 47649089486     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-008-9107-7     Document Type: Article

References (26)

1. Anholt R, Lindstrom J, Montal M (1980) Functional equivalence of monomeric and dimeric forms of purified acetylcholine receptors from Torpedo californica in reconstituted lipid vesicles. Eur J Biochem 109:481-487
2. Anholt R, Lindstrom J, Montal M (1981) Stabilization of acetylcholine receptor channels by lipids in cholate solution and during reconstitution in vesicles. J Biol Chem 256:4377-4387
3. Bhushan A, McNamee MG (1990) Differential scanning calorimetry and Fourier transform infrared analysis of lipid-protein interactions involving the nicotinic acetylcholine receptor. Biochim Biophys Acta 1027:93-101
4. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911-917
5. Boheim G, Hanke W, Barrantes FJ, Eibl H, Sakmann B, Fels G, Maelicke A (1981) Agonist-activated ionic channels in acetylcholine receptor reconstituted into planar lipid bilayers. Proc Natl Acad Sci USA 78:3586-3590
6. Cheng A, Leung A, Fellmann D, Quispe J, Suloway C, Pulokas J, Abeyrathne PD, Lam JS, Carragher B, Potter CS (2007) Towards automated screening of two-dimensional crystals. J Struct Biol 160:324-331
7. da Costa CJ, Ogrel AA, McCardy EA, Blanton MP, Baenziger JE (2002) Lipid-protein interactions at the nicotinic acetylcholine receptor. A functional coupling between nicotinic receptors and phosphatidic acid-containing lipid bilayers. J Biol Chem 277:201-208
8. Dellisanti CD, Yao Y, Stroud JC, Wang ZZ, Chen L (2007) Crystal structure of the extracellular domain of nAChR alpha1 bound to alpha-bungarotoxin at 1.94 Å resolution. Nat Neurosci 10:953-962; erratum in Nat Neurosci (2007) 10:1222
9. Fong TM, McNamee MG (1986) Correlation between acetylcholine receptor function and structural properties of membranes. Biochemistry 25:830-840
10. Hamouda AK, Sanghvi M, Sauls D, Machu TK, Blanton MP (2006) Assessing the lipid requirements of the Torpedo californica nicotinic acetylcholine receptor. Biochemistry 45:4327-4337
11. Hertling-Jaweed S, Bandini G, Müller-Fahrnow A, Dommes V, Hucho F (1988) Rapid preparation of the nicotinic acetylcholine receptor for crystallization in detergent solution. FEBS Lett 241:29-32
12. Hilf RJ, Dutzler R (2008) X-ray structure of a prokaryotic pentameric ligand-gated ion channel. Nature 452:375-379
13. Jones OT, Eubanks JH, Earnest JP, McNamee MG (1988) A minimum number of lipids are required to support the functional properties of the nicotinic acetylcholine receptor. Biochemistry 27:3733-3742
14. Karlin A (2002) Emerging structure of the nicotinic acetylcholine receptors. Nat Rev Neurosci 3:102-114
15. Karlin A, Akabas MH (1995) Toward a structural basis for the function of nicotinic acetylcholine receptors and their cousins. Neuron 15:1231-1244
16. Martínez KL, Gohon Y, Corringer PJ, Tribet C, Mérola F, Changeaux JP, Popot JL (2002) Allosteric transitions of Torpedo acetylcholine receptor in lipids, detergent and amphipols: molecular interactions vs. physical constraints. FEBS Lett 528:251-256
17. Miyazawa A, Fujiyoshi Y, Unwin N (2003) Structure and gating mechanism of the acetylcholine receptor pore. Nature 423:949-955
18. Nelson N, Anholt R, Lindstrom J, Montal M (1980) Reconstitution of purified acetylcholine receptors with functional ion channels in planar lipid bilayers. Proc Natl Acad Sci USA 77:3057-3061
19. Ochoa EL, Dalziel AW, McNamee MG (1983) Reconstitution of acetylcholine receptor function in lipid vesicles of defined composition. Biochim Biophys Acta 727:151-162
20. Paraschos A, González-Ros JM, Martínez-Carrión M (1982) Acetylcholine receptor from Torpedo. Preferential solubilization and efficient reintegration into lipid vesicles. Biochim Biophys Acta 691:249-260
21. Potter CS, Pulokas J, Smith P, Suloway C, Carragher B (2004) Robotic grid loading system for a transmission electron microscope. J Struct Biol 146:431-440
22. Schindler H, Quast U (1980) Functional acetylcholine receptor from Torpedo marmorata in planar membranes. Proc Natl Acad Sci USA 77:3052-3056
23. Schürholz T, Kehne J, Gieselmann A, Neumann E (1992) Functional reconstitution of the nicotinic acetylcholine receptor by CHAPS dialysis depends on the concentrations of salt, lipid, and protein. Biochemistry 31:5067-5077
24. Suloway C, Pulokas J, Fellmann D, Cheng A, Guerra F, Quispe J, Stagg S, Potter CS, Carragher B (2005) Automated molecular microscopy: the new Leginon system. J Struct Biol 151:41-60
25. Unwin N (2005) Refined structure of the nicotinic acetylcholine receptor at 4 Å resolution. J Mol Biol 346:967-989
26. Walker JW, Takeyasu K, McNamee MG (1982) Activation and inactivation kinetics of Torpedo californica acetylcholine receptor in reconstituted membranes. Biochemistry 21:5384-5389