Serratia marcescens internalization and replication in human bladder epithelial cells

BMC Infectious Diseases

Volumn 4, Issue , 2004, Pages

  Hertle, Ralf ^a   Schwarz, Heinz ^b  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLYSIN; GENTAMICIN; HEMOLYSIN; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; SHLA PROTEIN, SERRATIA MARCESCENS;

ACTIN FILAMENT; ARTICLE; BACTERIAL CELL; BACTERIAL INFECTION; BACTERIAL STRAIN; BACTERIUM ADHERENCE; BACTERIUM MUTANT; BLADDER EPITHELIUM; CELL ASSAY; CELL CULTURE; CELL DISRUPTION; CELL DIVISION; CELL INVASION; CELL MOTILITY; CELL TRANSPORT; CELL VACUOLE; CHANNEL GATING; CONTROLLED STUDY; CYTOLYSIS; CYTOTOXICITY; ELECTRON MICROSCOPY; EPITHELIUM CELL; EUKARYOTIC CELL; HEP 2 CELL; HUMAN; HUMAN CELL; MICROTUBULE; MONOLAYER CULTURE; NONHUMAN; PATHOGENESIS; PROTEIN SECRETION; SERRATIA MARCESCENS; SITE DIRECTED MUTAGENESIS; WILD TYPE; BIOSYNTHESIS; BLADDER; CELL LINE; CYTOLOGY; DRUG EFFECT; GENETICS; HEMOLYSIS; MICROBIOLOGY; PATHOGENICITY; PHYSIOLOGY; TRANSMISSION ELECTRON MICROSCOPY; TUMOR CELL LINE; VIRULENCE;

ANTI-BACTERIAL AGENTS; BACTERIAL ADHESION; BACTERIAL PROTEINS; CELL LINE; CELL LINE, TUMOR; EPITHELIAL CELLS; GENTAMICINS; HEMOLYSIN PROTEINS; HEMOLYSIS; HUMANS; MICROSCOPY, ELECTRON, TRANSMISSION; MUTAGENESIS, SITE-DIRECTED; SERRATIA MARCESCENS; URINARY BLADDER; VACUOLES; VIRULENCE;

EID: 4744359205     PISSN: 14712334     EISSN: None     Source Type: Journal    
DOI: 10.1186/1471-2334-4-16     Document Type: Article

References (39)

1. Allison C, Coleman N, Jones PL, Hughes C: Ability of Proteus mirabilis to invade human urothelial cells is coupled to motility and swarming differentiation. Infect Immun 1992, 60: 4740-4746.
2. Amako K, Ohshima H, Yasunaka K, Kono K: Pili mediated agglutination of Serratia marcescens in human urine. Microbiol Immunol 1981, 25:981-992.
3. Assadian O, Berger A, Aspock C, Mustafa S, Kohlhauser C, Hirschl AM: Nosocomial outbreak of Serratia marcescens in a neonatal intensive care unit. Infect Control Hosp Epidemiol 2002, 23:457-461.
4. Baorto DM, Gao Z, Malaviya R, Dustin ML, van der Merwe A, Lublin DM, Au AS: Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic. Nature 1997, 389: 36-639.
5. Bar-Ness R, Rosenberg M: Putative role of a 70 kDa outer-surface protein in promoting cell-surface hydrophobicity of Serratia marcescens RZ. J Gen Microbiol 1989, 135: 277-2281.
6. Bernheimer AW: Assay of hemolytic toxins. Meth Enzymol 1988, 165:213-217.
7. Coria-Jimenez R, Romero-Olvera J, Celis-Cruz C: Superficial hydrophobicity in Serratia marcescens strains of clinical origin. Res Microbiol 1998, 49:27-29.
8. Esel D, Doganay M, Bozdemir N, Yildiz O, Tezcaner T, Sumerkan B, Aygen B, Selcuklu A: Polymicrobial ventriculitis and evaluation of an outbreak in a surgical intensive care unit due to inadequate sterilization. J Hosp Infect 2002, 50:170-174.
9. Finlay BB, Falkow S: Common themes in microbial pathogenicity revisited. Microbiol Mol Biol Rev 1997, 61: 36-169.
10. Finlay BB, Falkow S: Comparison of the invasion strategies used by Salmonella cholerae-suis, Shigella flexneri and Yersinia enterocolitica to enter cultured animal cells:endosome acidification is not required for bacterial invasion or intracellular replication. Biochimie 1988, 70:1089-1099.
11. Fleisch F, Zimmermann-Baer U, Zbinden R, Bischoff G, Arlettaz R, Waldvogel K, Nadal D, Ruef C: Three consecutive outbreaks of Serratia marcescens in a neonatal intensive care unit. Clin Infect Dis 2002, 34:767-773.
12. Hejazi A, Falkiner FR: Serratia marcescens. J Med Microbiol 1997, 46:903-912.
13. Herrero M, de Lorenzo V, Timmis KN: Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria J Bacteriol 1990, 172:6557-6567.
14. Hertle R: Adhesion of Serratia marcescens to epithelial cells by type I fimbriae and hydrophobicity. 2004 in press.
15. Hertle R, Hilger M, Weingardt-Kocher S, Walev I: Cytotoxic action of Serratia marcescens hemolysin on human epithelial cells Infect Immun 1999, 67:817-825.
16. Hume EB, Conerly LL, Moreau JM, Cannon BM, Engel LS, Stroman DW, Hill JM, O'Callaghan RJ: Serratia marcescens keratitis: strainspecific corneal pathogenesis in rabbits. Curr Eye Res 1999, 19:525-532.
17. Janda JM, Abbott SL, Oshiro LS: Penetration and replication of Edwardsiella spp. in HEp-2 cells. Infect Immun 1991, 59:154-161.
18. König W, Faltin Y, Scheffer J, Schöffler H, Braun V: Role of cellbound hemolysin as a pathogenicity factor for Serratia infections. Infect Immun 1987, 55:2554-2561.
19. Kreft B, Carstensen O, Straube E, Bohnet S, Hacker J, Marre R: Adherence to and cytotoxicity of Escherichia coli for eucaryotic cell lines quantified by MTT [3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide]. Int J Med Microbiol Virol Parasitol Infect Dis 1992, 276: 31-242.
20. Kurz CL, Chauvet S, Andres E, Aurouze M, Vallet I, Michel GP, Uh M, Celli J, Filloux A, De Bentzmann S, Steinmetz I, Hoffmann JA, Finlay BB, Gorvel JP, Ferrandon D, Ewbank JJ: Virulence factors of the human opportunistic pathogen Serratia marcescens identified by in vivo screening. EMBO J 2003, 22:1451-1460.
21. Lee CA, Jones BD, Falkow S: Identification of a Salmonella typhimurium invasion locus by selection for hyperinvasive mutants. Proc Natl Acad Sci USA 1992, 89:1847-1851.
22. Leranoz S, Orus P, Berlanga M, Dalet F, Vinas M: New fimbrial adhesins of Serratia marcescens isolated from urinary tract infections: description and properties. J Urol 1997, 157:694-698.
23. Mallick SA: Cell surface hydrophobicity and its relation to outer membrane proteins of Serratia marcescens. Indian J Exp Biol 1996, 34:107-110.
24. Marre R, Hacker J, Braun J: The cell-bound hemolysin of Serratia marcescens contributes to uropathogenicity. Microbial Pathogenesis 1989, 7:153-156.
25. Miller VL, Mekalanos JJ: A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J Bacteriol 1988, 170:2575-2583.
26. Mizunoe Y, Matsumoto T, Haraoka M, Sakumoto M, Kubo S, Kumazawa J: Effect of pili of Serratia marcescens on superoxide production and phagocytosis of human polymorphonuclear leukocytes. J Urol 1995, 154:1227-1230.
27. Oelschlaeger TA, Tall BD: Uptake pathways of clinical isolates of Proteus mirabilis into human epithelial cell lines. Microb Pathogh 1996, 21:1-16.
28. Ondraczek R, Hobbie S, Braun S: In vitro activation of the Serratia marcescens hemolysin through modification and complementation. J Bacteriol 1992, 174:5086-5094.
29. Ostrowsky BE, Whitener C, Bredenberg HK, Carson LA, Holt S, Hutwagner L, Arduino MJ, Jarvis WR: Serratia marcescens bacteremia traced to an infused narcotic. N Engl J Med 2002, 346:1529-1537.
30. Poole K, Braun V: Influence of growth temperature and lipopolysaccharide on hemolytic activity of Serratia marcescens. J Bacteriol 1988, 170:5146-5152.
31. Ruan Y, Braun V: Hemolysin as a marker for Serratia Arch Microbiol 1990, 154:221-225.
32. Schiebel E, Braun V: Integration of the Serratia marcescens haemolysin into human erythrocyte membranes. Mol Microbiol 1989, 3:445-453.
33. Schmitz G, Braun V: Cell-bound and secreted proteases of Serratia marcescens. J Bacteriol 1985, 161: 002-1009.
34. Sieben S, Hertle R, Gumpert J, Braun V: Expression and secretion of Serratia marcescens hemolysin in stable protoplast type Lforms of Proteus mirabilis. Arch Microbiol 1998, 170: 36-242.
35. Strauss EJ, Ghori N, Falkow S: An Edwardsiella tarda strain containing a mutation in a gene with homology to shlB and hpmB is defective for entry into epithelial cells in culture. Infect Immun 1997, 65:3924-3932.
36. Studier FW, Moffatt BA: Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 1986, 189:113-130.
37. Vieira J, Messing J: The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 1982, 19:259-268.
38. Wood GE, Dutro SM, Totten PA: Target cell range of Haemophilus ducreyi hemolysin and its involvement in invasion of human epithelial cells. Infect Immun 1999, 67:3740-3749.
39. Yamamoto T, Ariyoshi A, Amako K: Fimbria-mediated adherence of Serratia marcescens strain US5 to human urinary bladder surface. Microbiol Immunol 1985, 29:677-681.