Genotoxicity of lead in lupin root cells as evaluated by the comet assay

Cellular and Molecular Biology Letters

Volumn 9, Issue 3, 2004, Pages 519-528

  Rucińska, Renata ^a   Sobkowiak, Robert ^a   Gwóźdź, Edward A ^a  

^a ADAM MICKIEWICZ UNIVERSITY   (Poland)

Author keywords

Comet Assay; Lead; Lupin

Indexed keywords

DNA FRAGMENT; LEAD; PLANT DNA;

ARTICLE; CELL NUCLEUS; COMET ASSAY; CONCENTRATION (PARAMETERS); DNA DETERMINATION; ELECTRIC FIELD; EVALUATION; EXPOSURE; GENOTOXICITY; LUPIN; NONHUMAN; PH; PLANT ROOT; ROOT CELL; ROOT GROWTH; SEEDLING;

LUPINUS; LUPINUS LUTEUS;

EID: 4744344922     PISSN: 14258153     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Wierzbicka, M. How lead loses its toxicity to plants. Acta Soc. Bot. Pol. 64 (1995) 81-90.
2. Rank, J. and Nielsen, M.N. Evaluation of Allium cepa anaphase-telophase test in relation to genotoxicity screening of industrial wastewater. Mutat. Res. 312 (1994) 17-24.
3. Steinkellner, H., Mun-Sik, K., Helma, C., Ecker, S., Ma, T.H., Horak, O., Kundi, M. and Knasmü, S. Genotoxic effects of heavy metals: comparative investigation with plant bioassays. Environ. Mol. Mutagen. 31 (1998) 183-191.
4. Conte, C., Mutti, I., Puglisi, P., Ferrarini, A., Regina, G., Maestri, E. and Marmoroli, N. DNA fingerprinting analysis by a PCR based method for monitoring the genotoxic effects of heavy metals pollution. Chemosphere 37 (1998) 2739-2749.
5. Kovalchuk, O., Titiv, V., Hohn, B. and Kovalchuk, I. A sensitive transgenic plant system to detect toxic inorganic compounds in the environment. Nat. Biotech. 19 (2001) 568-572.
6. Fairbairn, D.W., Olive, P.L. and O'Neill, K.L. The comet assay: a comprehensive review. Mutat. Res. 339 (1995) 37-59.
7. Koppen, G. and Verschaeve, L. The alkaline comet test on plant cell: A new genotoxicity test for DNA strand breaks in Viciafaba root cells. Mutat Res. 360 (1996) 193-200.
8. Rojas, E., Lopez, M.C. and Valverde, M. Single gel electophoresis assay: methodology and application. J. Chromatogr. 722 (1999) 225-254.
9. Rucińska, R., Waplak, S. and Gwóźdź, E.A. Free radical formation and activity of antioxidant enzymes in lupin roots exposed to lead. Plant Physiol. Biochem. 37 (1999) 187-194.
10. 2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79 (1994) 583-593.
11. Pfosser, A., Amon, A., Lelley, T. and Heberle-Bors, E. Evaluation of sensitivity of flow cytometry in detecting aneuploidy in wheat using disomic and ditelosomic wheat-rye addition lines. Cytometry 21 (1995) 387-393.
12. Nadin, S.B., Vargas-Roing, L.M. and Ciocca, D.R. A silver staining method for single-cell gel assay. J. Histochem. Cytochem. 49 (2001) 1183-1186.
13. Helma, C. and Uhl, M. A public domain image-analysis program for the single-cell gel-electrophoresis (comet) assay. Mutat. Res. 466 (2000) 9-15.
14. Ashby, J.A., Tinwell, H., Levefre, P.A. and Browne, M.A. The single gel electrophoresis assay for induced DNA damage (comet assay): measurement tail length and moment. Mutagenesis 10 (1995) 85-90.
15. Collins, A.R., Duthie, S.J. and Dobson, V.L. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis 14 (1993) 1733-1735.
16. Przymusiński R., Spychała M., Gwóźdź E.A. Inorganic lead changes growth and polypeptide pattern of lupin roots. Biochem. Physiol. Pflanzen 187 (1991) 51-57.
17. Przymusiński R., Gwóźdź E.A. Heavy metal-induced polypeptides in lupin roots are similar to pathogenesis-related proteins. J. Plant Physiol. 154 (1999) 703-708.
18. Yang, Y.Y., Jung, J.Y., Song, W.Y., Suh, H.S. and Lee, Y. Identification of rice varieties with high tolerance or sensitivity to lead and characterization on the mechanism of tolerance. Plant Physiol. 124 (2000) 1019-1026.
19. Ernst H.O., Effects of metals in plants at the cellular and organismic level. in: Ecotoxicology, (Schüürmann, G. and Market, M., Eds), J. Wiley and Sons, Inc. And Spektrum Akademischer Verlag, Heidelberg, 1998, 588-620.
20. Woźniak, K. and Blasiak, J. In vitro genotoxicity of lead acetate: induction of single and double strand breaks and DNA-protein cross-links. Mutat. Res. 535 (2003) 127-139.
21. Zhang, Y. and Yang, X. The toxic effects of cadmium on cell division and chromosomal morphology of Hordeum vulgare. Mutat. Res. 312 (1994) 121-126.
22. Cunningham, R.P. DNA repair: caretakers of the genome? Curr. Biol. 7 (1997) R576-R579.
23. Yang, J.L., Wang L.C., Chang C.Y. and Liu, T.Y. Singlet oxygen is the major species participating in the induction of DNA strand breakage and 8-hydroxydeoxyguanosine adduct by lead acetate. Mol. Mutagen. 33 (1999) 194-201.