Maize recombinant non-C4 NADP-malic enzyme: A novel dimeric malic enzyme with high specific activity

Plant Molecular Biology

Volumn 55, Issue 1, 2004, Pages 97-107

  Saigo, Mariana ^a   Bologna, Federico P ^a   Maurino, Verónica G ^b   Detarsio, Enrique ^a   Andreo, Carlos S ^a   Drincovich, María F ^a  

^a UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

^b UNIVERSITY OF COLOGNE   (Germany)

Author keywords

C4 evolution; NADP malic enzyme; Non photosynthetic; Oligomeric state; Prokariotic expression; Zea mays

Indexed keywords

CROPS; DNA; ESCHERICHIA COLI; METABOLISM; OLIGOMERS; PHOTOSYNTHESIS; PLANTS (BOTANY);

IMMUNOREACTIVE BANDS; ISOFORMS; METABOLIC PATHWAYS;

ENZYMES;

COMPLEMENTARY DNA; ISOENZYME; MALATE DEHYDROGENASE; MALATE DEHYDROGENASE (OXALOACETATE DECARBOXYLATING) (NADP+); MALATE DEHYDROGENASE (OXALOACETATE-DECARBOXYLATING) (NADP+); RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; DIMERIZATION; DNA SEQUENCE; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; MAIZE; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; WESTERN BLOTTING;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CLONING, MOLECULAR; DIMERIZATION; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; ISOENZYMES; KINETICS; MALATE DEHYDROGENASE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; ZEA MAYS;

CORN; ENZYMES; EVOLUTION; METABOLISM; NUCLEIC ACIDS; OLIGOMERS; PHOTOSYNTHESIS; PLANTS;

ESCHERICHIA COLI; ZEA MAYS;

EID: 4744337931     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-004-0472-z     Document Type: Article

References (22)

1. 3). Phytochemistry 52: 985-992.
2. Chang, G.-G. and Tong, L. 2003. Structure and function of malic enzyme, a new class of oxidative decarboxylase. Biochemistry 42: 12721-12733.
3. 4 NADP-malic enzyme: expression in E. coli and characterization of site-directed mutants at the putative nucleotide binding sites. J. Biol. Chem. 278: 13757-13764
4. Drincovich, M.F., Iglesias, A.A. and Andreo, C.S. 1991. Interaction of divalent metal ions with the NADP-malic enzyme from maize leaves. Plant Physiol. 81: 462-466.
5. 4 photosynthesis in Flaveria species. Isoforms of NADP-malic enzyme. Plant Physiol. 117: 733-744.
6. Drincovich, M.F., Casati, P. and Andreo, C.S. 2001. NADP-malic enzyme from plants: A ubiquitous enzyme involved in different metabolic pathways. FEBS Lett. 290: 1-6.
7. Edwards, G.E. and Andreo, C.S. 1992. NADP-malic enzyme from plants. Phytochemistry 31: 1845-1857.
8. Emanuelsson, O., Nielsen, H. and von Heijne, G. 1999. ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci. 8: 978-984.
9. Emanuelsson, O., Nielsen, H., Brunak, S. and von Heijne, G. 2000. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J. Mol. Biol. 300: 1005-1016.
10. Huang, T.-M. and Chang, G.-G. 1992. Characterization of the tetramer-dimer-monomer equilibrium of the enzymatically active subunits of pigeon liver malic enzyme. Biochemistry 31: 12658-12664.
11. Iglesias, A.A. and Andreo, C.S. 1990. NADP-dependent malate dehydrogenase (decarboxylating) from sugar cane leaves. Kinetic properties of different oligomeric structures. Eur. J. Biochem. 192: 729-732.
12. 4. Nature 227: 680-685.
13. 4 Flaveria species. Plant Physiol. 128: 140-149.
14. 4 Flaveria species. Plant Physiol. 128: 125-139.
15. Maurino, V.G., Drincovich, M.F. and Andreo, C.S. 1996. NADP-malic enzyme isoforms in maize leaves. Biochem. Mol. Biol. Int. 38: 239-250.
16. 3 plant wheat. J. Exp. Bot. 48: 799-811.
17. Maurino, V.G., Saigo, M., Andreo, C.S. and Drincovich, M.F. 2001. Non-photosynthetic NADP-malic enzyme from maize: A constitutively expressed enzyme that responds to plant defense inducers. Plant Mol. Biol. 45: 409-420.
18. Plaxton, W.C. 1989. Molecular and immunological characterization of plastid and cytosolic pyruvate kinase isozymes from castor-oil-plant endosperm and leaf. Eur. J. Biochem. 181: 443-451.
19. Takeuchi, Y., Akagi, H., Kasamura, N., Osumi, M. and Honda, H. 1998. Aberrant chloroplasts in transgenic rice expressing high level of maize NADP-dependent malic enzyme. Planta. 211: 265-274.
20. 4 dependent malic enzymes are encoded by distinct genes derived from a plastid-localized ancestor. Plant Mol. Biol. 50: 635-652.
21. Xu, Y., Bhargava, G., Wu, H., Loeber, G. and Tong, L. 1999. Crystal structure of human mitochondrial NAD(P) dependent malic enzyme: a new class of oxidative decarboxylases. Structure 7: 877-889.
22. Yang, Z., Zhang, H., Hung, H.-H., Kuo, C.-C., Tsai, L.-C., Yuan, H.S., Chou, W.Y., Chang, G.-G. and Tong, L. 2002. Structural studies of the pigeon liver cytosolic NADP-dependent malic enzyme. Prot. Sci. 11: 332-341.