Characterization of rabbit ear skin as a skin model for in vitro transdermal permeation experiments: Histology, lipid composition and permeability

Skin Pharmacology and Physiology

Volumn 21, Issue 4, 2008, Pages 218-226

  Nicoli, S ^a   Padula, C ^a   Aversa, V ^a   Vietti, B ^a   Wertz, P W ^b   Millet, A ^c   Falson, F ^c   Govoni, P ^a   Santi, P ^a  

^a UNIVERSITY OF PARMA   (Italy)

^b UNIVERSITY OF IOWA   (United States)

^c UNIVERSITÉ LYON 1   (France)

Author keywords

Differential Scanning Calorimetry; Lipid composition; Pig skin; Rabbit skin; Stratum corneum permeability; Transdermal permeation

Indexed keywords

CAFFEINE; CERAMIDE; CHOLESTEROL ESTER; NICOTINAMIDE; PROGESTERONE; TRIACYLGLYCEROL;

ANIMAL TISSUE; ARTICLE; DIFFERENTIAL SCANNING CALORIMETRY; DRUG PENETRATION; EAR; EPIDERMIS; HISTOLOGY; HYDROPHILICITY; IN VITRO STUDY; LIPID COMPOSITION; LIPOPHILICITY; NONHUMAN; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RABBIT; SKIN PERMEABILITY; STRATUM CORNEUM; SWINE;

ADMINISTRATION, CUTANEOUS; ANIMALS; CAFFEINE; CALORIMETRY, DIFFERENTIAL SCANNING; EPIDERMIS; LIPIDS; MODELS, BIOLOGICAL; NIACINAMIDE; PROGESTERONE; RABBITS; SKIN; SKIN ABSORPTION; SPECIES SPECIFICITY; SWINE;

EID: 47249112934     PISSN: 16605527     EISSN: None     Source Type: Journal    
DOI: 10.1159/000135638     Document Type: Article

References (42)

1. Asbill C, Kim N, El-Kattan A, Creek K, Wertz P, Michniak B: Evaluation of a human bio-engineered skin equivalent for drug permeation studies. Pharm Res 2000;17:1092-1097.
2. Schmook FP, Meingassner JG, Billich A: Comparison of human skin or epidermis models with human and animal skin in in vitro percutaneous absorption. Int J Pharm 2001;215:51-56.
3. Netzlaff F, Lehr CM, Wertz PW, Schaefer UF: The human epidermis models EpiSkin, SkinEthic and EpiDerm: an evaluation of morphology and their suitability for testing phototoxicity, irritancy, corrosivity, and substance transport. Eur J Pharm Biopharm 2005;60:167-178.
4. de Jager MW, Gooris GS, Dolbnya IP, Ponec M, Bouwstra JA: Modelling the stratum corneum lipid organisation with synthetic lipid mixtures: the importance of synthetic ceramide composition. Biochim Biophys Acta 2004;1664:132-140.
5. de Jager MW, Gooris GS, Ponec M, Bouwstra JA: Lipid mixtures prepared with well-defined synthetic ceramides closely mimic the unique stratum corneum lipid phase behavior. J Lipid Res 2005;46:2649-2656.
6. Panchagnula R, Stemmer K, Ritschel WA: Animal models for transdermal drug delivery. Methods Find Exp Clin Pharmacol 1997;19:335-341.
7. Wester RC, Maibach HI: Animal models for percutaneous absorption; in Shah VP, Maibach HI (eds): Topical Drug Bioavailability, Bioequivalence and Penetration. New York, Plenum Press, 1993, pp 333-349.
8. Sekkat N, Kalia YN, Guy RH: Biophysical study of porcine ear skin in vitro and its comparison to human skin in vivo. J Pharm Sci 2002;91:2376-2381.
9. Dick IP, Scott RC: Pig ear skin as an in-vitro model for human skin permeability. J Pharm Pharmacol 1992;44:640-645.
10. Simon GA, Maibach HI: The pig as an experimental animal model of percutaneous permeation in man: qualitative and quantitative observations - an overview. Skin Pharmacol Appl Skin Physiol 2000;13:229-234.
11. Andega S, Kanikkannan N, Singh M: Comparison of the effect of fatty alcohols on the permeation of melatonin between porcine and human skin. J Control Release 2001;77:17-25.
12. Hirvonen J, Rytting JH, Paronen P, Urtti A: Dodecyl N,N-dimethylamino acetate and azone enhance drug penetration across human, snake, and rabbit skin. Pharm Res 1991;8:933-937.
13. Corbo M, Liu JC, Chien YW: Transdermal controlled delivery of propranolol from a multilaminate adhesive device. Pharm Res 1989;6:753-758.
14. Kanikkannan N, Singh J, Ramarao P: In vitro transdermal iontophoretic transport of timolol maleate: effect of age and species. J Control Release 2001;71:99-105.
15. Artusi M, Nicoli S, Colombo P, Bettini R, Sacchi A, Santi P: Effect of chemical enhancers and iontophoresis on thiocolchicoside permeation across rabbit and human skin in vitro. J Pharm Sci 2004;93:2431-2438.
16. Nicoli S, Cappellazzi M, Colombo P, Santi P: Characterization of the permselective properties of rabbit skin during transdermal iontophoresis. J Pharm Sci 2003;92:1482-1488.
17. Nicoli S, Colombo P, Santi P: Release and permeation kinetics of caffeine from bioadhesive transdermal films. AAPS J 2005;7:E218-E223.
18. Nicoli S, Rimondi S, Colombo P, Santi P: Physical and chemical enhancement of transdermal delivery of triptorelin. Pharm Res 2001;18:1634-1637.
19. Nicoli S, Santi P: Suitability of excised rabbit ear skin - fresh and frozen - for evaluating transdermal permeation of estradiol. Drug Deliv 2007;14:195-199.
20. Ostacolo C, Marra F, Laneri S, Sacchi A, Nicoli S, Padula C, Santi P: Alpha-tocopherol pro-vitamins: synthesis, hydrolysis and accumulation in rabbit ear skin. J Control Release 2004;99:403-413.
21. Scott RC, Corrigan MA, Smith F, Mason H: The influence of skin structure on permeability: an intersite and interspecies comparison with hydrophilic penetrants. J Invest Dermatol 1991;96:921-925.
22. Jacobi U, Kaiser M, Toll R, Mangelsdorf S, Audring H, Otberg N, Sterry W, Lademann J: Porcine ear skin: an in vitro model for human skin. Skin Res Technol 2007;13:19-24.
23. Jacobi U, Taube H, Schäfer UF, Sterry W, Lademann J: Comparison of four different in vitro systems to study the reservoir capacity of the stratum corneum. J Control Release 2005;103:61-71.
24. Otberg N, Richter H, Schaefer H, Blume-Peytavi U, Sterry W, Lademann J: Variations of hair follicle size and distribution in different body sites. J Invest Dermatol 2004;122:14-19.
25. Lu MY, Lee D, Rao GS: Percutaneous absorption enhancement of leuprolide. Pharm Res 1992;9:1575-1579.
26. Law S, Wertz PW, Swartzendruber DC, Squier CA: Regional variation in content, composition and organization of porcine epithelial barrier lipids revealed by thin-layer chromatography and transmission electron microscopy. Arch Oral Biol 1995;40:1085-1091.
27. Silva CL, Nunes SC, Eusebio ME, Pais AA, Sousa JJ: Thermal behaviour of human stratum corneum: a differential scanning calorimetry study at high scanning rates. Skin Pharmacol Physiol 2006;19:132-139.
28. Silva CL, Nunes SC, Eusebio ME, Sousa JJ, Pais AA: Study of human stratum corneum and extracted lipids by thermomicroscopy and DSC. Chem Phys Lipids 2006;140:36-47.
29. Bleck O, Abeck D, Ring J, Hoppe U, Vietzke JP, Wolber R, Brandt O, Schreiner V: Two ceramide subfractions detectable in Cer(AS) position by HPTLC in skin surface lipids of non-lesional skin of atopic eczema. J Invest Dermatol 1999;113:894-900.
30. Lampe MA, Burlingame AL, Whitney J, Williams ML, Brown BE, Roitman E, Elias PM: Human stratum corneum lipids: characterization and regional variations. J Lipid Res 1983;24:120-130.
31. Moser K, Kriwet K, Naik A, Kalia YN, Guy RH: Passive skin penetration enhancement and its quantification in vitro. Eur J Pharm Biopharm 2001;52:103-112.
32. Hammond SA, Tsonis C, Sellins K, Rushlow K, Scharton-Kersten T, Colditz I, Glenn GM: Transcutaneous immunization of domestic animals: opportunities and challenges. Adv Drug Deliv Rev 2000;43:45-55.
33. Inan S, Oztukcan S, Vatansever S, Ermertcan AT, Zeybek D, Oksal A, Giray G, Muftuoglu S: Histopathological and ultrastructural effects of glycolic acid on rat skin. Acta Histochem 2006;108:37-47.
34. van den Bergh BA, Bouwstra JA, Junginger HE, Wertz PW: Elasticity of vesicles affects hairless mouse skin structure and permeability. J Control Release 1999;62:367-379.
35. Grubauer G, Feingold KR, Harris RM, Elias PM: Lipid content and lipid type as determinants of the epidermal permeability barrier. J Lipid Res 1989;30:89-96.
36. Tanojo H, Bouwstra JA, Junginger HE, Bodde HE: Thermal analysis studies on human skin and skin barrier modulation by fatty acids and propylene glycol. J Therm Anal Cal 1999;57:313-322.
37. Tanojo H, Bouwstra JA, Junginger HE, Bodde HE: In vitro human skin barrier modulation by fatty acids: skin permeation and thermal analysis studies. Pharm Res 1997;14:42-49.
38. Francoeur ML, Golden GM, Potts RO: Oleic acid: its effects on stratum corneum in relation to (trans)dermal drug delivery. Pharm Res 1990;7:621-627.
39. Hirvonen J, Rajala R, Vihervaara P, Laine E, Paronen P, Urtti A: Mechanism and reversibility of penetration enhancer action in the skin - a DSC study. Eur J Pharm Biopharm 1994;40:81-85.
40. Al-Saidan SM, Barry BW, Williams AC: Differential scanning calorimetry of human and animal stratum corneum membranes. Int J Pharm 1998;168:17-22.
41. Pouliot R, Germain L, Auger FA, Tremblay N, Juhasz J: Physical characterization of the stratum corneum of an in vitro human skin equivalent produced by tissue engineering and its comparison with normal human skin by ATR-FTIR spectroscopy and thermal analysis (DSC). Biochim Biophys Acta 1999;1439:341-352.
42. Dias M, Farinha A, Faustino E, Hadgraft J, Pais J, Toscano C: Topical delivery of caffeine from some commercial formulations. Int J Pharm 1999;182:41-47.