Rapid detection of pathogenic Vibrio parahaemolyticus by a sensitive and specific duplex PCR-hybridization probes assay using LightCycler

Journal of Applied Microbiology

Volumn 105, Issue 2, 2008, Pages 575-584

  Lo, C L H ^a   Leung, P H M ^a   Yip, S P ^a   To, T S S ^a   Ng, T K ^b   Kam, K M ^c  

^a HONG KONG POLYTECHNIC UNIVERSITY   (Hong Kong)

^b PRINCESS MARGARET HOSPITAL   (Hong Kong)

^c DEPARTMENT OF HEALTH   (Hong Kong)

Author keywords

Hybridization probe; LightCycler; Real time PCR; tdh; Thermostable direct haemolysin; toxR; Vibrio parahaemolyticus

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; BLOOD; POLYMERASE CHAIN REACTION; PROBES;

HAEMOLYSINS; HYBRIDIZATION PROBE; LIGHTCYCLER; PATHOGENICS; REAL TIME POLYMERASE CHAIN REACTIONS; TDH; THERMOSTABLE DIRECT; THERMOSTABLE DIRECT HEMOLYSIN; TOXR; VIBRIO PARAHAEMOLYTICUS;

GENES;

FLUORESCEIN; HEMOLYSIN;

BACTERIUM; BIOASSAY; BIOCHEMISTRY; DETECTION METHOD; PATHOGEN; POLYMERASE CHAIN REACTION; PROBE; REAL TIME; SENSITIVITY ANALYSIS;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BACTERIUM ISOLATE; COLONY FORMING UNIT; GENE AMPLIFICATION; GENE TARGETING; HYBRIDIZATION; LATEX AGGLUTINATION TEST; MISMATCH REPAIR; MOLECULAR PROBE; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; THERMOSTABILITY; UNINDEXED SEQUENCE; VIBRIO PARAHAEMOLYTICUS;

VIBRIO PARAHAEMOLYTICUS;

EID: 47249098507     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2008.03776.x     Document Type: Article

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