Real-time quantitative analysis of carbon catabolite derepression of cellulolytic genes expressed in the basidiomycete Phanerochaete chrysosporium

Applied Microbiology and Biotechnology

Volumn 80, Issue 1, 2008, Pages 99-106

  Suzuki, Hitoshi ^a   Igarashi, Kiyohiko ^a   Samejima, Masahiro ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Carbon catabolite derepression; Cellulose degradation; Phanerochaete chrysosporium; Real time PCR

Indexed keywords

BAST FIBERS; CARBON; CONCENTRATION (PROCESS); ENZYMES; GENE EXPRESSION; GENES; RATE CONSTANTS;

CARBON CATABOLITE DEREPRESSION; CARBON CATABOLITE REPRESSION; CATABOLITE DEREPRESSION; CELLOBIOHYDROLASES; CELLOBIOSE DEHYDROGENASE; CELLULOLYTIC ENZYMES; CELLULOSE DEGRADATION; CHRYSOSPORIUM; DE-REPRESSION; EXPRESSION LEVELS; GLUCOSE CONCENTRATIONS; GLUCOSIDASE; PHANEROCHAETE CHRYSOSPORIUM; PROMOTER ACTIVITIES; QUANTITATIVE ANALYSIS; REAL-TIME PCR; REVERSE TRANSCRIPTASE- POLYMERASE CHAIN REACTION;

GLUCOSE;

BETA GLUCOSIDASE; CARBON; CELLOBIOSE QUINONE OXIDOREDUCTASE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; GLUCOSE;

CARBON; CATABOLISM; CELLULOSE; FUNGUS; GENE EXPRESSION; GLUCOSE; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME;

ARTICLE; BASIDIOMYCETES; CATABOLITE REPRESSION; FUNGUS CULTURE; GENE EXPRESSION; INCUBATION TIME; MYCELIUM; NUCLEOTIDE SEQUENCE; PHANEROCHAETE; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CELLULOSE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GLUCOSE; PHANEROCHAETE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSCRIPTION, GENETIC;

CARBON; ENZYMES; GENES; GLUCOSE; QUANTITATIVE ANALYSIS; TISSUE CULTURE;

PHANEROCHAETE CHRYSOSPORIUM;

EID: 47149090525     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1539-6     Document Type: Article

References (43)

1. Aro N, Pakula T, Penttila M (2005) Transcriptional regulation of plant cell wall degradation by filamentous fungi. FEMS Microbiol Rev 29:719-739
2. Ayers AR, Ayers SB, Eriksson KE (1978) Cellobiose oxidase, purification and partial characterization of a hemoprotein from Sporotrichum pulverulentum. Eur J Biochem 90:171-181
3. Bao W, Usha SN, Renganathan V (1993) Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungus Phanerochaete chrysosporium. Arch Biochem Biophys 300:705-713
4. Broda P, Birch PR, Brooks PR, Sims PF (1995) PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families. Appl Environ Microbiol 61:2358-2364
5. Canevascini G, Coudray M-R, Rey J-P, Southgate RJG, Meier H (1979) Induction and catabolite repression of cellulase synthesis in the thermophilic fungus Sporotrichum thermophile. J Gen Microbiol 110:291-303
6. Covert SF, Vanden Wymelenberg A, Cullen D (1992) Structure, organization, and transcription of a cellobiohydrolase gene cluster from Phanerochaete chrysosporium. Appl Environ Microbiol 58:2168-2175
7. Cubero B, Scazzocchio C (1994) Two different, adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans. EMBO J 13:407-415
8. Deshpande V, Eriksson KE, Pettersson B (1978) Production, purification and partial characterization of 1,4-beta-glucosidase enzymes from Sporotrichum pulverulentum. Eur J Biochem 90:191-198
9. Dowzer CE, Kelly JM (1989) Cloning of the creA gene from Aspergillus nidulans: a gene involved in carbon catabolite repression. Curr Genet 15:457-459
10. Dowzer CE, Kelly JM (1991) Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans. Mol Cell Biol 11:5701-5709
11. Eberhart BM, Beck RS, Goolsby KM (1977) Cellulase of Neurospora crassa. J Bacteriol 130:181-186
12. Eriksson KE (1978) Enzyme mechanisms involved in cellulose hydrolysis by the rot fungus Sporotrichum pulverulentum. Biotechnol Bioeng 20:317-332
13. Henriksson G, Sild V, Szabo IJ, Pettersson G, Johansson G (1998) Substrate specificity of cellobiose dehydrogenase from Phanerochaete chrysosporium. Biochim Biophys Acta 1383:48-54
14. Igarashi K, Tani T, Kawai R, Samejima M (2003) Family 3 beta-glucosidase from cellulose-degrading culture of the white-rot fungus Phanerochaete chrysosporium is a glucan 1,3-beta-glucosidase. J Biosci Bioeng 95:572-576
15. Ilmen M, Thrane C, Penttila M (1996a) The glucose repressor gene cre1 of Trichoderma: isolation and expression of a full-length and a truncated mutant form. Mol Gen Genet 251:451-460
16. Ilmen M, Onnela ML, Klemsdal S, Keranen S, Penttila M (1996b) Functional analysis of the cellobiohydrolase I promoter of the filamentous fungus Trichoderma reesei. Mol Gen Genet 253:303-314
17. Ilmen M, Saloheimo A, Onnela ML, Penttila ME (1997) Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol 63:1298-1306
18. Johnsrud SC, Eriksson KE (1985) Cross-breeding of selected and mutated homokaryotic strains of Phanerochaete chrysosporium K-3-new cellulase deficient strains with increased ability to degrade lignin. Appl Microbiol Biotechnol 21:320-327
19. Kremer SM, Wood PM (1992) Evidence that cellobiose oxidase from Phanerochaete chrysosporium is primarily an Fe(III) reductase. Kinetic comparison with neutrophil NADPH oxidase and yeast flavocytochrome b2. Eur J Biochem 205:133-138
20. Kurasawa T, Yachi M, Suto M, Kamagata Y, Takao S, Tomita F (1992) Induction of cellulase by gentiobiose and its sulfur-containing analog in Penicillium purpurogenum. Appl Environ Microbiol 58:106-110
21. Lamar RT, Schoenike B, Vanden Wymelenberg A, Stewart P, Dietrich DM, Cullen D (1995) Quantitation of fungal mRNAs in complex substrates by reverse transcription PCR and its application to Phanerochaete chrysosporium-colonized soil. Appl Environ Microbiol 61:2122-2126
22. Li B, Nagalla SR, Renganathan V (1996) Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium. Appl Environ Microbiol 62:1329-1335
23. Lymar ES, Li B, Renganathan V (1995) Purification and characterization of a cellulose-binding (beta)-glucosidase from cellulose-degrading cultures of Phanerochaete chrysosporium. Appl Environ Microbiol 61:2976-2980
24. Mandels M, Parrish FW, Reese ET (1962) Sophorose as an inducer of cellulase in Trichoderma viride. J Bacteriol 83:400-408
25. Meijer MM, Boonstra J, Verkleij AJ, Verrips CT (1998) Glucose repression in Saccharomyces cerevisiae is related to the glucose concentration rather than the glucose flux. J Biol Chem 273:24102-24107
26. Ohnishi Y, Nagase M, Ichiyanagi T, Kitamoto Y, Aimi T (2007) Transcriptional regulation of two cellobiohydrolase encoding genes (cel1 and cel2) from the wood-degrading basidiomycete Polyporus arcularius. Appl Microbiol Biotechnol 76:1069-78
27. Rho D, Desrochers M, Jurasek L, Driguez H, Defaye J (1982) Induction of cellulose in Schizophyllum commune: thiocellobiose as a new inducer. J Bacteriol 149:47-53
28. Schmoll M, Kubicek CP (2003) Regulation of Trichoderma cellulase formation: lessons in molecular biology from an industrial fungus. A review. Acta Microbiol Immunol Hung 50:125-145
29. Seiboth B, Hakola S, Mach RL, Suominen PL, Kubicek CP (1997) Role of four major cellulases in triggering of cellulase gene expression by cellulose in Trichoderma reesei. J Bacteriol 179:5318-5320
30. Sims P, James C, Broda P (1988) The identification, molecular cloning and characterisation of a gene from Phanerochaete chrysosporium that shows strong homology to the exo-cellobiohydrolase I gene from Trichoderma reesei. Gene 74:411-422
31. Sims PF, Soares-Felipe MS, Wang Q, Gent ME, Tempelaars C, Broda P (1994) Differential expression of multiple exo-cellobiohydrolase I-like genes in the lignin-degrading fungus Phanerochaete chrysosporium. Mol Microbiol 12:209-216
32. Smith MH, Gold MH (1979) Phanerochaete chrysosporium beta-glucosidases: Induction, cellular localization, and physical characterization. Appl Environ Microbiol 37:938-942
33. Stapleton PC, Dobson AD (2003) Carbon repression of cellobiose dehydrogenase production in the white rot fungus Trametes versicolor is mediated at the level of gene transcription. FEMS Microbiol Lett 221:167-172
34. Strauss J, Mach RL, Zeilinger S, Hartler G, Stoffler G, Wolschek M, Kubicek CP (1995) Cre1, the carbon catabolite repressor protein from Trichoderma reesei. FEBS Lett 376:103-107
35. Streamer M, Eriksson KE, Pettersson B (1975) Extracellular enzyme-system utilized by fungus Sporotrichum-Pulverulentum (Chrysosporium-Lignorum) for breakdown of cellulose - Functional characterization of 5 endo-1,4-beta- glucanases and one exo-1,4-beta-glucanase. Eur J Biochem 59:607-613
36. Takashima S, Iikura H, Nakamura A, Masaki H, Uozumi T (1996) Analysis of Cre1 binding sites in the Trichoderma reesei cbh1 upstream region. FEMS Microbiol Lett 145:361-366
37. Tempelaars CA, Birch PR, Sims PF, Broda P (1994) Isolation, characterization, and analysis of the expression of the cbhII gene of Phanerochaete chrysosporium. Appl Environ Microbiol 60:4387-4393
38. Uzcategui E, Ruiz A, Montesino R, Johansson G, Pettersson G (1991) The 1,4-beta-D-glucan cellobiohydrolases from Phanerochaete-Chrysosporium. 1. A system of synergistically acting enzymes homologous to Trichoderma-Reesei. J Biotechnol 19:271-285
39. Vallim MA, Janse BJ, Gaskell J, Pizzirani-Kleiner AA, Cullen D (1998) Phanerochaete chrysosporium cellobiohydrolase and cellobiose dehydrogenase transcripts in wood. Appl Environ Microbiol 64:1924-1928
40. Vanden Wymelenberg A, Covert SF, Cullen D (1993) Identification of the gene encoding the major cellobiohydrolase of the white rot fungus Phanerochaete chrysosporium. Appl Envir Microbiol 59:3492-3494
41. Vanden Wymelenberg A, Minges P, Sabat G et al (2006) Computational analysis of the Phanerochaete chrysosporium v2.0 genome database and mass spectrometry identification of peptides in ligninolytic cultures reveal complex mixtures of secreted proteins. Fungal Genet Biol 43:343-356
42. Yoshida M, Igarashi K, Kawai R, Aida K, Samejima M (2004) Differential transcription of beta-glucosidase and cellobiose dehydrogenase genes in cellulose degradation by the basidiomycete Phanerochaete chrysosporium. FEMS Microbiol Lett 235:177-182
43. Zeilinger S, Schmoll M, Pail M, Mach RL, Kubicek CP (2003) Nucleosome transactions on the Hypocrea jecorina (Trichoderma reesei) cellulase promoter cbh2 associated with cellulase induction. Mol Genet Genomics 270:46-55