Highly efficient secretion of heterologous proteins from Saccharomyces cerevisiae using inulinase signal peptides

Biotechnology and Bioengineering

Volumn 49, Issue 4, 1996, Pages 473-479

  Chung, Bong Hyun ^a   Nam, Soo Wan ^b   Kim, Byung Moon ^a   Park, Young Hoon ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Dong Eui University   (South Korea)

Author keywords

human interleukin 2; human lipocortin 1; INU signal peptide; MF 1 leader peptide; secretion

Indexed keywords

INSULINASE; LIPOCORTIN 1; RECOMBINANT INTERLEUKIN 2;

AMINO ACID SEQUENCE; ARTICLE; CELL CULTURE; NONHUMAN; PEPTIDE SYNTHESIS; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE;

AMINO ACIDS; CELL CULTURE; GENES; VECTORS; YEAST;

HUMAN INTERLEUKIUM; HUMAN LIPOCORTIN; INULINASE SIGNAL PEPTIDES; KLUYVEROMYCES MARXIANUS; SACCHAROMYCES CEREVISIAE; SECRETION;

PROTEINS;

EID: 0030085133     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960220)49:4<473::AID-BIT15>3.0.CO;2-B     Document Type: Article

References (29)

1. Achstetter, T., Nguyen-Juilleret, M., Findeli, A., Merkamm, M., Lemoine, Y. 1992. A new signal peptide useful for secretion of heterologous proteins from yeast and its application for synthesis of hirudin. Gene 110: 25-31.
2. Baldari, C., Murray, J. A. H., Ghiara, P., Cesareni, G., Galeotti, C. L. 1987. A novel leader peptide which allows efficient secretion of a fragment of human interleukin 1 β in Saccharomyces cerevisiae. EMBO J. 6: 229-234.
3. Bitter, G. A., Chen, K. K., Banks, A. R., Lai, P.-H. 1984. Secretion of foreign proteins from Saccharomyces cerevisiae directed by α-factor gene fusions. Proc. Natl. Acad. Sci. USA 81: 5330-5334.
4. Cabezon, T., De Wilde, M., Herion, P., Loriau, R., Bollen, A. 1984. Expression of human α1-antitrypsin cDNA in the yeast Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 81: 6594-6598.
5. Choi, E.-S., Sohn, J.-H., Rhee, S.-K. 1994. Optimization of expression system using galactose inducible promoter for the production of anticoagulant hirudin in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 42: 587-594.
6. Caung, B. H., Seo, D. J., Park, Y. H., Lee, S. B., Han, M. H. 1991. Production of recombinant human interleukin-2 by Escherichia coli with computer controlled temperature induction. Biotechnol. Techniq. 5: 163-168.
7. Cohen, S. N., Chiang, A. C. Y., Hsu, L. 1972. Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of E. coli by R factor DNA. Proc. Natl. Acad. Sci. USA 69: 2110-2114.
8. De Nobel, L. G., Barnett, J. A. 1991. Passage of molecules through yeast cell walls: A brief essay-review. Yeast 7: 313-323.
9. Emr, S. D., Schekman, R., Flessel, M. C., Thorner, J. 1983. An MFα1-SUC2 (α-factor-invertase) gene fusion for study of protein localization and gene expression in yeast. Proc. Natl. Acad. Sci. USA 80: 7080-7084.
10. Giga-Hama, Y., Tohda, H., Okada, H., Owada, M. K., Okayama, H., Kumagai, H. 1994. High-level expression of human lipocortin I in the fission yeast Schizosaccharomyces pombe using a novel expression vector. Bio/Technology 12: 400-404.
11. Hinnen, A., Meyhack, B , Heim, J. 1989. Heterologous gene expression in yeast, pp. 193-213. In P. J. Barr, A. J. Brake, and P. Valenzuela (eds.), Yeast genetic engineering. Butterworths, London.
12. Hitzeman, R. A., Lenny, D. W., Petty, L. J., Kohr, W. J., Levine, H. L., Goeddel, D. V. 1983. Secretion of human interferons by yeast. Science 219: 620-625.
13. Hofmann, K. J., Schultz, L. D. 1991. Mutations of the α-galactosidase signal peptide which greatly enhance secretion of heterologous proteins by yeast. Gene 101: 105-111.
14. Ito, H., Fukuda, Y., Murata, K., Kimura, A. 1983. Transformation of intact yeast cell treated with alkali cations. J. Bacteriol. 153: 163-168.
15. Julius, D., Brake, A., Blair, L., Kunisawa, R., Thorner, J. 1984. Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor. Cell 37: 1075-1089.
16. Kim, B. .M., Nam, S. W., Chung, B. H., Park, Y. H., Rhee, S. K. 1994. Effect of transcription terminators on expression of human lipocortin-1 in recombinant Saccharomyces cerevisiae. J. Microbiol Biotechnol. 4: 237-244.
17. Ko, J. H. 1991. Studies on the extracellular secretion of proteins in yeast, Ph.D. thesis, The University of Tokyo, Tokyo, Japan.
18. Laloux, O., Cassart, J. P., Delcour, J., van Beeumen, J., Vandenhaute, J. 1991. Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424. FEBS Lett. 289: 64-68.
19. Moir, D. T., Dumais, D. R. 1987. Glycosylation and secretion of human α-1-antitrypsin by yeast. Gene 56: 209-217.
20. Nam, S. W., Kim, B. M., Chung, B. H., Kang, D. O., Ahn, J. S. 1994. Expression and secretion of human lipocortin-1 by promoter and signal sequence of STA1 from Saccharomyces diastaticus. Biotechnol. Lett. 16: 897-902.
21. Nam, S. W., Yoda, K., Yamasaki, M. 1993. Secretion and localization of invertase and inulinase in recombinant Saccharomyces cerevisiae. Biotechnol. Lett. 15: 1049-1054.
22. Rholam, M., Nicolas, P., Cohen, P. 1986. Precursors for peptide hormones share common secondary structures forming features at the proteolytic processing sites. FEBS Lett. 207: 1-6.
23. Sambrook, J., Fritsch, E. F., Maniatis, T. 1989. Molecular cloning: A laboratory manual. 2nd edition. Cold Spring Harbor Laboratory, Cold Spring, New York.
24. Sanger, F., Nicklen, S., Coulson, A. R. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467.
25. Sleep, D., Belfield, G. P., Goodey, A. R. 1991. The secretion of human serum albumin from the yeast Saccharomyces cerevisiae using five different leader sequences. Bio/Technol. 8: 42-46.
26. Smith, R. A., Duncan, M. J., Moir, D. T. 1985. Heterologous protein secretion from yeast. Science 229: 1219-1224.
27. Taniguchi, T., Mitsui, H., Fujita, T., Takaoka, C., Kashima, N., Yoshimoto, R., Hamuro, J. 1983. Structure and expression of a cloned cDNA for human interleukin-2. Nature 302: 305-310.
28. von Heijne, G. 1984. How signal sequences maintain cleavage specificity. J. Mol. Biol. 173: 243-251.
29. Zsebo, K. M., Lu, H.-S., Fieschko, J. C., Goldstein, L., Davis, J., Duker, K., Suggs, S. V., Lai, P.-H., Bitter, G. A. 1986. Protein secretion from Saccharomyces cerevisiae directed by the prepro-α-factor leader region. J. Biol. Chem. 261: 5858-5865.