Green fluorescent protein (GFP) as a direct biosensor for mutation detection: Elimination of false-negative errors in target gene expression

Analytical Biochemistry

Volumn 380, Issue 1, 2008, Pages 91-98

  Tak, Yu Kyung ^a   Naoghare, Pravin K ^a   Lee, Kyeong Hee ^b   Park, Soon Sup ^c   Song, Joon Myong ^a  

^a Seoul National University   (South Korea)

^b Korea Electrotechnology Research Institute (KERI)   (South Korea)

^c Korea Electronic Technology Institute (KETI)   (South Korea)

Author keywords

Direct biosensor; Green fluorescent protein; Mutagenic efficacy

Indexed keywords

BIOSENSORS; CELL CULTURE; DNA; FLUORESCENCE; MUTAGENESIS; PROTEINS;

ADDUCT FORMATION; FALSE NEGATIVE ERRORS; GREEN FLUORESCENT PROTEIN; MUTAGENIC EFFECT; MUTAGENIC EFFICACY; MUTATION DETECTION; SHUTTLE VECTOR; TARGET GENE EXPRESSION;

GENE EXPRESSION;

GREEN FLUORESCENT PROTEIN; MUTAGENIC AGENT;

ARTICLE; BIOSENSOR; CELL CULTURE; CONFOCAL MICROSCOPY; ERROR; FALSE POSITIVE RESULT; FLOW CYTOMETRY; GENE EXPRESSION; MUTAGENESIS; MUTATION; PRIORITY JOURNAL;

EUKARYOTA;

EID: 46849112854     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.05.029     Document Type: Article

References (27)

1. Grubor N.M., Shinar R., Jankowiak R., Porter M.D., and Small G.J. Novel biosensor chip for simultaneous detection of DNA-carcinogen adducts with low-temperature fluorescence. Biosens. Bioelectron. 19 (2004) 547-556
2. Rowe-Taitt C.A., Golden J.P., Feldstein M.J., Cras J.J., Hoffman K.E., and Ligler F.S. Array biosensor for detection of biohazards. Biosens. Bioelectron. 14 (2000) 785-794
3. Maher V.M., Yang J.L., Mah M.C., and McCormick J.J. Comparing the frequency and spectra of mutations induced when an SV-40 based shuttle vector containing covalently bound residues of structurally-related carcinogens replicates in human cells. Adv. Exp. Med. Biol. 220 (1989) 83-92
4. Dycaico M.J., Provost G.S., Kretz P.L., Ransom S.L., Moores J.C., and Short J.M. The use of shuttle vectors for mutation analysis in transgenic mice and rats. Mutat. Res. 307 (1994) 461-478
5. Jones M., Burkhart-Schultz K., and Carrano A.V. A method to quantify spontaneous and in vivo induced thioguanine-resistant mouse lymphocytes. Mutat. Res. 147 (1985) 97-105
6. Bigger C.A., Ponten I., Page J.E., and Dipple A. Mutational spectra for polycyclic aromatic hydrocarbons in the supF target gene. Mutat. Res. 450 (2000) 75-93
7. Yang L., Maher V.M., and McCormick J.J. Kinds of mutations formed when a shuttle vector containing adducts of (±)-7beta, 8alpha-dihydroxy-9alpha, 10alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene replicates in human cells. Proc. Natl. Acad. Sci. USA 84 (1987) 3787-3791
8. Wani M.A., Zhu Q., El-Mahdy M., Venkatachalam S., and Wani A.A. Enhanced sensitivity to anti-benzo(a)pyrene-diol-epoxide DNA damage correlates with decreased global genomic repair attributable to abrogated p53 function in human cells. Cancer Res. 60 (2000) 2273-2280
9. Mukhopadhyay S., Clark D.R., Watson N.B., Zacharias W., and McGregor W.G. REV1 accumulates in DNA damage-induced nuclear foci in human cells and is implicated in mutagenesis by benzo[a]pyrenediolepoxide. Nucleic Acids Res. 32 (2004) 5820-5826
10. Stanisławska-Sachadyn A., Sachadyn P., Ihle K., Sydorczuk C., Wiejacha K., and Kur J. The construction of bifunctional fusion proteins consisting of MutS and GFP. J. Biotechnol. 121 (2006) 134-143
11. Heim R., Cubitt A.B., and Tsien R.Y. Improved green fluorescence. Nature 373 (1995) 663-664
12. Heim R., and Tsien R.Y. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6 (1996) 178-182
13. Cariello N.F., Narayanan S., Kwanyuen P., Muth H., and Casey W.M. A novel bacterial reversion and forward mutation assay based on green fluorescent protein. Mutat. Res. 414 (1998) 95-105
14. Yu M., Chalfie T., Guskirchen G., Ward W.W., and Parsher D.C. Green fluorescent protein as a marker for gene expression. Science 263 (1994) 802-805
15. Kohler S.W., Provost G.S., Fieck A., Kretz P.L., Bullock W.O., Sorge J.A., Levy D.D., Groopman J.D., Lim S.E., Seidman M.M., and Kraemer K.H. Spectra of spontaneous and mutagen-induced mutations in the lacI gene in transgenic mice. Cancer Res. 52 (1992) 5668-5673
16. Denissenko M.F., Cahill J., Koudriakova T.B., Gerber N., and Pfeifer G.P. Quantitation and mapping of aflatoxin B1-induced DNA damage in genomic DNA using aflatoxin B1-8,9-epoxide and microsomal activation systems. Mutat. Res. 425 (1999) 205-211
17. Geacintov N.E., Zinger D., Ibanez V., Santella R., Grunberger D., and Harvey R.G. Properties of covalent benzo[a]pyrene diol epoxide-DNA adducts investigated by fluorescence techniques. Carcinogenesis 8 (1987) 925-935
18. Chary P., and Lloyd R.S. In vitro replication by prokaryotic and eukaryotic polymerases on DNA templates containing site-specific and stereospecific benzo[a]pyrene-7, 8-dihydrodiol-9, 10-epoxide adducts. Nucleic Acids Res. 23 (1995) 1398-1405
19. Billinton N., Barker M.G., Michel C.E., Knight A.W., Heyer W.-D., Goddard N.J., Fielden P.R., and Walmsley R.M. Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor. Biosens. Bioelectron. 13 (1998) 831-838
20. Hsiang Y.H., Hertzberg R., Hecht S., and Liu L.F. Camptothecin induced protein-linked DNA breaks via mammalian DNA topoisomerase I. J. Biol. Chem. 260 (1985) 14873-14878
21. Jaxel C., Kohn K.W., and Yves P. Topoisomerase I interaction with SV4O DNA in the presence and absence of camptothecin. Nucleic Acids Res. 16 (1988) 11157-11170
22. Porter S.E., and Champoux J.J. The basis for camptothecin enhancement of DNA breakage by eukaryotic topoisomerase I. Nucleic Acids Res. 17 (1989) 8521-8532
23. Tanizawa K., Kohn K.W., Kohlhagen G., Leteurtre F., and Pommier Y. Differential stabilization of eukaryotic DNA topoisomerase I cleavable complexes by camptothecin derivatives. Biochemistry 34 (1995) 7200-7206
24. Pommier Y., Kohlhagen G., Pourquier P., Sayer J.M., Kroth H., and Jerina D.M. Benzo[a]pyrene diol epoxide adducts in DNA are potent suppressors of a normal topoisomerase I cleavage site and powerful inducers of other topoisomerase I cleavages. Proc. Natl. Acad. Sci. USA 97 (2000) 2040-2045
25. Lodovici M., Luceri C., Guglielmi F., Bacci C., Akpan V., Fonnesu M.L., Boddi V., and Dolara P. Benzo(a)pyrene diolepoxide (BPDE)-DNA adduct levels in leukocytes of smokers in relation to polymorphism of CYP1A1,GSTM1,GSTP1,GSTT1, and mEH. Cancer Epidemiol. Biomarkers Prev. 13 (2004) 1342-1348
26. Bartsch H., Nair U., Risch A., Rojas M., Wilkman H., and Alexandrov K. Genetic polymorphism of CYP genes, alone or in combination, as a risk modifier of tobacco-related cancers. Cancer Epidemiol. Biomarkers Prev. 9 (2000) 3-28
27. Ryberg D., Skaug V., Hewer A., David H., Lorna P., Harries W., Roland C., Wolf C.R., Øgreid D., Ulvik A., Vu P., and Haugen A. Genotypes of glutathione transferase M1 and P1 and their significance for lung DNA adduct levels and cancer risk. Carcinogenesis 18 (1997) 1285-1289