Detection and molecular characterization of Vibrio parahaemolyticus isolated from seafood harvested along the southwest coast of India

Food Microbiology

Volumn 25, Issue 6, 2008, Pages 824-830

  Raghunath, Pendru ^a   Acharya, Sadananda ^a   Bhanumathi, Amarbahadur ^a   Karunasagar, Iddya ^a   Karunasagar, Indrani ^a  

^a University   (India)

Author keywords

Colony hybridization; Genetic markers; PCR; Vibrio parahaemolyticus

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN; HEMOLYSIN; THERMOSTABLE DIRECT HEMOLYSIN RELATED HEMOLYSIN PROTEIN, VIBRIO PARAHAEMOLYTICUS; THERMOSTABLE DIRECT HEMOLYSIN-RELATED HEMOLYSIN PROTEIN, VIBRIO PARAHAEMOLYTICUS;

ANIMAL; ARTICLE; BACTERIAL COUNT; FOOD CONTAMINATION; GENETIC MARKER; GENETICS; HUMAN; INDIA; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; PREVALENCE; RISK ASSESSMENT; SAFETY; SEA FOOD; VIBRIO PARAHAEMOLYTICUS;

ANIMALS; BACTERIAL PROTEINS; COLONY COUNT, MICROBIAL; CONSUMER PRODUCT SAFETY; DNA, BACTERIAL; FOOD CONTAMINATION; GENETIC MARKERS; HEMOLYSIN PROTEINS; HUMANS; INDIA; POLYMERASE CHAIN REACTION; PREVALENCE; RISK ASSESSMENT; SEAFOOD; VIBRIO PARAHAEMOLYTICUS;

BACTERIA (MICROORGANISMS); VIBRIO PARAHAEMOLYTICUS;

EID: 46749131812     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2008.04.002     Document Type: Article

References (48)

1. In: Ausubel F.M., Brent R., Kingsten R.E., Moore D.D., Seidman J.G., Smith J.A., and Struhl K. (Eds). Short Protocols in Molecular Biology (1995), Wiley, New York, NY, pp. 2.11-2.12
2. Bhuiyan N.A., Ansaruzzaman M., Kamruzzaman M., Alam K., Chowdhury N.R., Nishibuchi M., Faruque S.M., Sack D.A., Takeda Y., and Nair G.B. Prevalence of the pandemic genotype of Vibrio parahaemolyticus in Dhaka, Bangladesh, and significance of its distribution across different serotypes. J. Clin. Microbiol. 40 (2002) 284-286
3. Cai Y.L., and Ni Y.X. Purification, characterization and pathogenicity of urease produced by Vibrio parahaemolyticus. J. Clin. Lab. Anal. 10 (1996) 70-73
4. Chowdhury N.R., Chakraborty S., Eampokalap B., Chaicumpa W., Chongsa-Nguan M., Moolasart P., Mitra R., Ramamurthy T., Bhattacharya S.K., Nishibuchi M., Takeda Y., and Nair G.B. Clonal dissemination of Vibrio parahaemolyticus displaying similar fingerprint but belonging to two different serovars (O3:K6 and O4:K68) in Thailand and India. Epidemiol. Infect. 125 (2000) 17-25
5. Chowdhury A., Ishibashi M., Thiem V.D., Tuyet D.T., Tung T.V., Chien B.T., Seidlein L.V., Canh D.G., Clemens J., Trach D.D., and Nishibuchi M. Emergence and serovar transition of Vibrio parahaemolyticus pandemic strains isolated during a diarrhea outbreak in Vietnam between 1997-1999. Microbiol. Immunol. 48 (2004) 319-327
6. Chun D., Chung J.K., Tak Y., and Seol S.Y. Nature of the Kanagawa phenomenon of Vibrio parahaemolyticus. Infect. Immun. 12 (1975) 81-87
7. Daniels N.A., Ray B., Easton A., Marano N., Kahn E., McShan A.L., Del Rosario L., Baldwin T., Kingsley M.A., Puhr N.D., Wells J.G., and Angulo F.J. Emergence of a new Vibrio parahaemolyticus serotype in raw oysters. JAMA 284 (2000) 1541-1545
8. Deb B.C., Sinha R., De S.P., Sengupta P.G., Sikda S.M., and Mondal A. Studies on Vibrio parahaemolyticus infection in Calcutta as compared to cholera infection. Prog. Diagn. Res. Trop. Dis. 19 (1975) 400-405
9. Deepanjali A., Sanath Kumar H., Karunasagar I., and Karunasagar I. Seasonal variation in abundance of total and pathogenic Vibrio parahaemolyticus in oysters along the southwest coast of India. Appl. Environ. Microbiol. 71 (2005) 3575-3580
10. DePaola A., Hopkins L.H., Peeler J.T., Wentz B., and McPhearson R.M. Incidence of Vibrio parahaemolyticus in US coastal waters and oysters. Appl. Environ. Microbiol. 56 (1990) 2299-2302
11. DePaola A., Nordstrom J.L., Bowers J.C., Wells J.G., and Cook D.W. Seasonal abundance of total and pathogenic Vibrio parahaemolyticus in Alabama oysters. Appl. Environ. Microbiol. 69 (2003) 1521-1526
12. Dileep V., Kumar H.S., Kumar Y., Nishibuchi M., Karunasagar I., and Karunasagar I. Application of polymerase chain reaction for detection of Vibrio parahaemolyticus associated with tropical seafoods and coastal environment. Lett. Appl. Microbiol. 36 (2003) 423-427
13. Food and Drug Administration, 2004. Bacteriological Analytical Manual On Line, eighth ed., Revision A, 1998. AOAC International, Arlington, VA (Chapter 9) 〈http://www.cfsan.fda.gov/~ebam/bam-9.html〉.
14. Fujino T., Okuno Y., Nakada D., Aoyama A., Fukai K., Mukai T., and Ueho T. On the bacteriological examination of shellfish food poisoning. Med. J. Osaka Univ. 4 (1953) 299-305
15. Hara-Kudo Y., Sugiyama K., Nishibuchi M., Chowdhury A., Yatsuyanagi J., Ohtomo Y., Saito A., Nagano H., Nishina T., Nakagawa H., Konuma H., Miyahara M., and Kumagai S. Prevalence of pandemic thermostable direct hemolysin-producing Vibrio parahaemolyticus O3:K6 in seafood and the coastal environment in Japan. Appl. Environ. Microbiol. 69 (2003) 3883-3891
16. Hayashi S., Okura M., and Osawa R. Soft-agar coated filter method for early detection of viable and thermostable direct hemolysin (TDH) or TDH-related hemolysin producing Vibrio parahaemolyticus in seafood. Appl. Environ. Microbiol. 72 (2006) 4576-4582
17. Huq M.I., Huber D., and Kibryia G. Isolation of urease producing Vibrio parahaemolyticus strains from cases of gastroenteritis. Indian J. Med. Res. 70 (1979) 549-553
18. Iida T., Hattori A., Tagomori K., Nasu H., Naim R., and Honda T. Filamentous phage associated with recent pandemic strains of Vibrio parahaemolyticus. Emerg. Infect. Dis. 7 (2001) 477-478
19. Joseph S.W., Colwell R.R., and Kaper J.B. Vibrio parahaemolyticus and related halophilic vibrios. Crit. Rev. Microbiol. 10 (1982) 77-124
20. Kaneko T., and Colwell R.R. Ecology of Vibrio parahaemolyticus in Chesapeake Bay. J. Bacteriol. 113 (1973) 24-32
21. Karunasagar I., Venugopal M.N., Karunasagar I., and Segar K. Evaluation of methods for enumeration of Vibrio parahaemolyticus from seafood. Appl. Environ. Microbiol. 52 (1986) 583-585
22. Karunasagar I., Sugumar G., Karunasagar I., and Reilly P.J.A. Rapid polymerase chain reaction method for detection of Kanagawa positive Vibrio parahaemolyticus in seafoods. Int. J. Food Microbiol. 31 (1996) 317-323
23. Kaysner C.A., Abeyta C.J., Scott R.F., Krane M.H., and Wekell M.M. Enumeration of Vibrio species, including V. cholerae from samples of an oyster growing area, Grays Harbor, WA. J. Food Prot. 53 (1996) 300-302
24. Khan A.A., McCarthy S., Wang R.F., and Cerniglia C.E. Characterisation of United States outbreak isolates of Vibrio parahaemolyticus using enterobacterial repetitive intergenic consensus (ERIC) PCR and development of a rapid PCR method for detection of O3:K6 isolates. FEMS Microbiol. Lett. 206 (2002) 209-214
25. Kim Y.B., Okuda J., Matsumoto C., Takahashi N., Hashimoto S., and Nishibuchi M. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J. Clin. Microbiol. 37 (1999) 1173-1177
26. Matsumoto C., Okuda J., Ishibashi M., Iwanaga M., Garg P., Ramamurthy T., Wong H.C., DePaola A., Kim Y.B., Albert M.J., and Nishibuchi M. Pandemic spread of an O3:K6 clone of Vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed PCR and toxRS sequence analyses. J. Clin. Microbiol. 38 (2000) 578-585
27. McCarthy S.A., DePaola A., Cook D.W., Kaysner C.A., and Hill W.E. Evaluation of alkaline phosphatase- and digoxigenin-labeled probes for detection of the thermolabile hemolysin (tlh) gene of Vibrio parahaemolyticus. Lett. Appl. Microbiol. 28 (1999) 66-70
28. McCarthy S.A., DePaola A., Kaysner C.A., Hill W.E., and Cook D.W. Evaluation of non-isotopic DNA hybridization methods for detection of the tdh gene of Vibrio parahaemolyticus. J. Food. Prot. 63 (2000) 1660-1664
29. Meador C.E., Parsons M.M., Bopp C.A., Smidt P.G., Painter J.A., and Vora G.J. Virulence gene and pandemic group-specific marker profiling of clinical Vibrio parahaemolyticus isolates. J. Clin. Microbiol. 45 (2007) 1133-1139
30. Myers M.L., Panicker G., and Bej A.K. PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the Gulf of Mexico. Appl. Environ. Microbiol. 69 (2003) 2194-2200
31. Nair G.B., and Hormazabal J.C.O. The Vibrio parahaemolyticus pandemic. Rev. Chilena Infectol. 22 (2005) 125-130
32. Nasu H., Iida T., Sugahara T., Yamaichi Y., Park K.-S., Yokoyama K., Makino K., Shinagawa H., and Honda T. A filamentous phage associated with recent pandemic Vibrio parahaemolyticus O3:K6 strains. J. Clin. Microbiol. 38 (2000) 2156-2161
33. Nishibuchi M., and Kaper J.B. Thermostable direct hemolysin gene of Vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect. Immun. 63 (1995) 251-256
34. Nishibuchi M., Ishibashi M., Takeda Y., and Kaper J.B. Detection of thermostable direct hemolysin gene and related DNA sequences in Vibrio parahaemolyticus and other Vibrio spp. by DNA colony hybridization test. Infect. Immun. 49 (1985) 481-486
35. Nolan C.M., Ballad J., Kaysner C.A., Lilja J.L., Williams L.P., and Tenover F.C. Vibrio parahaemolyticus gastroenteritis an outbreak associated with raw oysters in the pacific Northwest. Diagn. Microbiol. Infect. Dis. 2 (1984) 119-128
36. Nordstrom J.L., and DePaola A. Improved recovery of pathogenic Vibrio parahaemolyticus from oysters using colony hybridization following enrichment. J. Microbiol. Meth. 52 (2003) 273-277
37. Okuda J., Ishibashi M., Hayakawa E., Nishino T., Takeda Y., Mukhopadhyay A.K., Garg S., Bhattacharya S.K., Nair G.B., and Nishibuchi M. Emergence of a unique O3:K6 clone of Vibrio parahaemolyticus in Calcutta, India and isolation of strains from same clonal group from South East Asian travellers arriving in Japan. J. Clin. Microbiol. 35 (1997) 3150-3155
38. Okura M., Osawa R., Iguchi A., Takagi M., Arakawa E., Terajima J., and Watanabe H. Genotypic analyses of Vibrio parahaemolyticus and development of a pandemic group-specific multiplex PCR assay. J. Clin. Microbiol. 41 (2003) 4676-4682
39. Okura M., Osawa R., Iguchi A., Takagi M., Arakawa E., Terajima J., and Watanabe H. PCR-based identification of pandemic group Vibrio parahaemolyticus with a novel group-specific primer pair. Microbiol. Immunol. 48 (2004) 787-790
40. Osawa R., Okitsu T., Morozumi H., and Yamai S. Occurrence of urease positive Vibrio parahemolyticus in Kanagawa, Japan, with specific reference to presence of thermo stable direct hemolysin (TDH) and the TDH-related hemolysin genes. Appl. Environ. Microbiol. 62 (1996) 725-727
41. Osawa R., Iguchi A., Arakawa E., and Watanabe H. Genotyping of pandemic Vibrio parahaemolyticus O3:K6 still open to question. J. Clin. Microbiol. 40 (2002) 2708-2709
42. Parvathi A., Sanath Kumar H., Bhanumathi A., Ishibashi M., Nishibuchi M., Karunasagar I., and Karunasagar I. Molecular characterization of thermostable direct haemolysin-related haemolysin (TRH)-positive Vibrio parahaemolyticus from oysters in Mangalore, India. Environ. Microbiol. 8 (2006) 997-1004
43. Raghunath P., Pradeep B., Karunasagar I., and Karunasagar I. Rapid detection and enumeration of trh-carrying Vibrio parahaemolyticus with the alkaline phosphatase-labeled oligonucleotide probe. Environ. Microbiol. 9 (2007) 266-270
44. Suthienkul O., Ishibashi M., Iida T., Nethip N., Supavej S., Eampolalap B., Makini M., and Honda T. Urease production correlated with possession of the trh gene in Vibrio parahaemolyticus strains isolated in Thailand. J. Infect. Dis. 172 (1995) 1405-1408
45. Tada J., Ohashi T., Nishimura N., Shirasaki Y., Ozaki H., Fukushima S., Takano J., Nishibuchi M., and Takeda Y. Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reaction. Mol. Cell. Probes 6 (1992) 477-487
46. Thompson C.A., and Vanderzant C. Serological and hemolytic characteristics of Vibrio parahaemolyticus from marine sources. J. Food Sci. 41 (1976) 204-205
47. Wagatsuma S. A medium for the test of the hemolytic activity of Vibrio parahaemolyticus. Media Circle 13 (1968) 159-162
48. Williams T.L., Musser S.M., Nordström J.L., DePaola A., and Monday S.R. Identification of a protein biomarker unique to the pandemic O3:K6 clone of Vibrio parahaemolyticus. J. Clin. Microbiol. 42 (2004) 1657-1665