PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the Gulf of Mexico

Applied and Environmental Microbiology

Volumn 69, Issue 4, 2003, Pages 2194-2200

  Myers, Michael L ^a,b   Panicker, Gitika ^a   Bej, Asim K ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DNA; WATER;

MICROBIAL SPECIES;

MICROBIOLOGY;

BACTERIAL DNA; GULF WATER; OLIGONUCLEOTIDE; PRIMER DNA; UNCLASSIFIED DRUG; WATER;

BACTERIUM; DETECTION METHOD; POLYMERASE CHAIN REACTION; SEAWATER;

ACCURACY; ARTICLE; BACTERIUM CULTURE; COASTAL WATERS; DILUTION; DNA DETERMINATION; EARLY DIAGNOSIS; ENVIRONMENTAL FACTOR; EPIDEMIC; FOOD SAFETY; GENE AMPLIFICATION; GEOGRAPHIC DISTRIBUTION; INCUBATION TIME; INFECTION RISK; MARINE ENVIRONMENT; METHODOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PATHOGENESIS; POLYMERASE CHAIN REACTION; SAMPLING; SEA FOOD; SENSITIVITY AND SPECIFICITY; SHIP; SPECIES IDENTIFICATION; STRAIN IDENTIFICATION; TEMPERATURE MEASUREMENT; VIBRIO PARAHAEMOLYTICUS; WATER POLLUTANT;

BACTERIAL TYPING TECHNIQUES; COMMUNICABLE DISEASES, EMERGING; CULTURE MEDIA; DISEASE OUTBREAKS; DNA PRIMERS; HUMANS; OPEN READING FRAMES; POLYMERASE CHAIN REACTION; SEAWATER; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; VIBRIO INFECTIONS; VIBRIO PARAHAEMOLYTICUS;

GULF OF MEXICO;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; VIBRIO; VIBRIO PARAHAEMOLYTICUS;

EID: 0037393043     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.4.2194-2200.2003     Document Type: Article

References (26)

1. Atlas, R. M. 1993. Handbook of microbiological media, p. 529. CRC Press, Inc., Boca Raton, Fla.
2. Atlas, R. M., and A. K. Bej. 1990. Detection of bacterial pathogens in environmental water samples by using polymerase chain reaction and gene probe, p. 399-406. In M. Innis, D. Gelfand, J. Sninsky, and T. White (ed.), A guide to methods and applications: a laboratory manual. Academic Press, Orlando, Fla.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Smith, J. G. Seidman, and K. Struhl (ed.). 1987. Current protocols in molecular biology, p. 2.10-2.11. John Wiley & Sons, Inc., New York, N.Y.
4. Bej, A. K., D. P. Patterson, C. W. Brasher, C. L. Vickery, D. D. Jones, and C. A. Kaysner. 1999. Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh. J. Microbiol. Methods 36:215-225.
5. Bej, A. K., M. H. Mahbubani, and R. M. Atlas. 1991. Amplification of nucleic acids by polymerase chain reaction and other methods. Crit. Rev. Biochem. Mol. Biol. 16:301-334.
6. Bhuiyan, N. A., M. Ansaruzzaman, M. Kamruzzaman, K. Alam, N. R. Chowdhury, M. Nishibuchi, S. M. Faruque, D. A. Sack, Y. Takeda, and G. B. Nair. 2002. Prevalence of the pandemic genotype of Vibrio parahaemolyticus in Dhaka, Bangladesh, and significance of its distribution across different serotypes. J. Clin. Microbiol. 40:284-286.
7. Center for Food Safety and Applied Nutrition, Food and Drug Administration, U.S. Department of Health and Human Services. 2000. Draft risk assessment on the public health impact of Vibrio parahaemolyticus in raw molluscan shellfish. Center for Food Safety and Applied Nutrition, Washington, D.C.
8. Centers for Disease Control and Prevention. 1999. Outbreak of Vibrio parahaemolyticus infections associated with eating raw oysters - Pacific Northwest, 1997. Morb. Mortal. Wkly. Rep. 47:457-462.
9. Chiou, C.-S., S.-Y. Hsu, S.-I. Chiu, T.-K. Wang, and C.-S. Chao. 2000. Vibrio parahaemolyticus serovar O3:K6 as cause of unusually high incidence of food-borne disease outbreaks in Taiwan from 1996 to 1999. J. Clin. Microbiol. 38:4621-4625.
10. Chowdhury, N. R., S. Chakraborty, T. Rammamurthy, M. Nishibuchi, S. Yamasaki, Y. Takeda, and G. B. Nair. 2000. Molecular evidence of clonal Vibrio parahaemolyticus pandemic strains. Emerg. Infect. Dis. 6:631-636.
11. Cordova, J. L., J. Astorga, W. Silva, and C. Riqueline. 2002. Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak. Biol. Res. 35:433-440.
12. Daniels, N. A., B. Ray, A. Easton, N. Marano, E. Kahn, A. L. McShan, L. Del Rosario, T. Baldwin, M. A. Kingsley, N. D. Puhr, J. G. Wells, and F. J. Angulo. 2000. Emergence of a new Vibrio parahaemolyticus serotype in raw oysters. JAMA 284:1541-1545.
13. DePaola, A., C. A. Kaysner, J. Bowers, and D. W. Cook, 2000. Environmental investigations of Vibrio parahaemolyticus in oysters after outbreaks in Washington, Texas, and New York (1997 and 1998). Appl. Environ. Microbiol. 66:4649-4654.
14. Gendel, S. M., J. Ulaszek, M. Nishubichi, and A. DePaola. 2001. Automated ribotyping differentiates Vibrio parahaemolyticus O3:K6 strains associated with a Texas outbreak from other clinical strains. J. Food Prot. 64:1617-1620.
15. Iida, T., A. Hattori, K. Tagomori, H. Nasu, R. Naim, and T. Honda. 2001. Filamentous phage associated with recent pandemic strains of Vibrio parahaemolyticus. Emerg. Infect. Dis. 7:477-478.
16. Khan, A. A., S. McCarthy, R. F. Wang, and C. E. Cerniglia. 2002. Characterization of United States outbreak isolates of Vibrio parahaemolyticus using enterobacterial repetitive intergenic consensus (ERIC) PCR and development of a rapid PCR method for detection of O3:K6 isolates. FEMS Microbiol. Lett. 206:209-214.
17. Matsumoto, C., J. Okuda, M. Ishibashi, M. Iwanaga, P. Garg, T. Rammamurthy, H.-C. Wong, A. Depaola, Y. B. Kim, M. J. Albert, and M. Nishibuchi. 2000. Pandemic spread of an O3:K6 clone of Vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed PCR and toxRS sequence analyses. J. Clin. Microbiol. 38:578-585.
18. McCarthy, S. A., A. DePaola, C. A. Kaysner, W. E. Hill, and D. W. Cook. 2000. Evaluation of nonisotopic DNA hybridization methods for detection of the tdh gene of Vibrio parahaemolyticus. J. Food Prot. 63:1660-1664.
19. Nasu, H., T. Iida, T. Sugahara, Y. Yamaichl, K.-S. Park, K. Yokoyama, K. Makino, H. Shinagawa, and T. Honda. 2000. A filamentous phage associated with recent pandemic Vibrio parahaemolyticus O3:K6 strains. J. Clin. Microbiol. 38:2156-2161.
20. Okuda, J., M. Ishibashi, E. Hayakawa, T. Nishino, Y. Takeda, A. K. Mukhopadhyay, S. Garg, S. K. Bhattacharya, G. B. Nair, and M. Nishibuchi. 1997. Emergence of a unique O3:K6 clone of Vibrio parahaemolyticus in Calcutta, India, and isolation of strains from the same clonal group from Southeast Asian travelers arriving in Japan. J. Clin. Microbiol. 35:3150-3155.
21. Rychilk, W., and R. E. Rhoads. 1989. A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA. Nucleic Acids Res. 17:8543-8551.
22. Smith, J. M., E. J. Feil, and N. H. Smith. 2000. Population structure and evolutionary dynamics of pathogenic bacteria. Bioessays 22:1115-1122.
23. Smolikova, L. M., I. M. Lomov, T. V. Khomenko, G. P. Murnachev, T. A. Kudriakova, O. P. Fetsailova, E. M. Sanamiants, L. D. Makedonova, G. V. Kachkina, and E. N. Golenishcheva. 2001. Studies on halophilic vibrios causing a food poisoning outbreak in the city of Vladivostok. Zh. Mikrobiol. Epidemiol. Immunobiol. 6:3-7.
24. Sparagano, O. A., P. A. Robertson, I. Purdom, J. McInnes, Y. Li, D. H. Yu, Z. J. Du, H. S. Xu, and B. Austin. 2000. PCR and molecular detection for differentiating Vibrio species. Ann. N. Y. Acad. Sci. 969:60-65.
25. Twedt, R. M. 1989. Vibrio parahaemolyticus, p, 552-554. In M. P. Doyle (ed.), Foodborne bacterial pathogens. Marcel Dekker Inc., New York, N.Y.
26. Waldor, M. K., and J. J. Mekalanos. 1996. Lysogenic conversion by a filamentous phage encoding cholera toxin. Science 272:1910-1914.