Genotyping of Legionella pneumophila serogroup 1 strains isolated in Northern Sicily, Italy

New Microbiologica

Volumn 31, Issue 2, 2008, Pages 217-228

  Chiarini, Alfredo ^a   Bonura, Celestino ^a   Ferraro, Donatella ^a   Barbaro, Roberta ^a   Calà, Cinzia ^a   Distefano, Salvatore ^a   Casuccio, Nicolò ^b   Belfiore, Santina ^b   Giammanco, Anna ^a  

^a UNIVERSITY OF PALERMO   (Italy)

^b AUSL 6   (Italy)

Author keywords

Amplified fragment length polymorphism (AFLP); Legionella pneumophila; Molecular typing; Sequence based typing (SBT); Surveillance; Water distribution system

Indexed keywords

BACTERIAL DNA; FRESH WATER;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM; ARTICLE; BACTERIUM IDENTIFICATION; CLASSIFICATION; CLUSTER ANALYSIS; DISINFECTION; DNA SEQUENCE; EPIDEMIOLOGY; GENETICS; GENOTYPE; ISOLATION AND PURIFICATION; ITALY; LEGIONELLA PNEUMOPHILA; METHODOLOGY; MICROBIOLOGY;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS; BACTERIAL TYPING TECHNIQUES; CLUSTER ANALYSIS; DISINFECTION; DNA, BACTERIAL; EPIDEMIOLOGY, MOLECULAR; FRESH WATER; GENOTYPE; LEGIONELLA PNEUMOPHILA; SEQUENCE ANALYSIS, DNA; SICILY;

LEGIONELLA PNEUMOPHILA; LEGIONELLA PNEUMOPHILA SEROGROUP 1;

EID: 46649121503     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. nd ed. Pittsburg, PA, 1-15.
2. AMEMURA-MAEKAWA J., KURA F., CHANG B., WATANABE H. (2005). Legionella pneumophila serogroup 1 isolates from cooling towers in Japan form a distinct genetic cluster. Microbiol. Immunol. 49, 1027-1033.
3. ANONYMOUS. (2000). Guidelines for prevention and control of legionellosis. Istituto Superiore di Sanità, Rome, Italy. Gazzetta Ufficiale n. 103/2000 May, 5. Available on the website http://.iss.it
4. BEST M., YU V.L., STOUT J., GOETZ A., MUDER R.R., TAYLOR F. (1983). Legionellaceae in the hospital water supply - epidemiological link with disease and evaluation of a method of control of nosocomial Legionnaires' disease and Pittsburgh pneumonia. Lancet. 2, 307-10.
5. BOOM R., SOL C.J.A., SALIMANS M.M.M., JANSEN C.L., WERTHEIM-VAN DILLEN P.M.E., VAN DER NORDAA J. (1990). Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28, 495-503.
6. BORELLA P., MONTAGNA M.P., STAMPI S., STANCANELLI G., ROMANO-SPICA V, TRIASSI M., MARCHESI I., BARGELLINI A., TATÒ D., NAPOLI C., ZANETTI F., LEONI E., MORO M., SCALTRITI S., RIBERA D'ALCALÀ G., SANTARPIA R., BOCCIA S. (2005). Legionella contamination in hot water of Italian hotels. Appl. Environ. Microbiol. 71, 5805-5813.
7. BORELLA P., MONTAGNA M.T., ROMANO-S PICA V, STAMPI S., STANCANELLI G., TRIASSI M., NEGLIA R., MARCHESI I., FANTUZZI G., TATÒ D., NAPOLI C., QUARANTA G., LAURENTI P., LEONI E., DE LUCA G., OSSI C., MORO M., RIBERA D'ALCALÀ G. (2004). Legionella infection risk from domestic hot water. Emerg. Infect. Dis. 10, 457-464.
8. CIANCIOTTO N.P. (2001). Pathogenicity of Legionella pneumophila. Int. J. Med. Microbiol. 291, 331-343.
9. EDELSTEIN P.H., CIANCIOTTO N.P. (2001). Legionella species and Legionnaires' disease. In: M. Dworkin, S. Falkow, E. Rosenberg, K.H. Schleifer and E. Stackebrandt (ed.). The prokaryotes: an evolving electronic resource for the microbiological community. Spinger-Verlag, New York, N. Y.
10. EWGLI (2005). European guidelines for control and prevention of travel associated Legionnaires' disease. Supplement IA. Technical guidelines for the control and prevention of Legionella in water systems, pp. 46-68. PHLS, London, United Kingdom. Available on the website http://.ewgli.org/data/ european-guidelines.htm.
11. th annual meeting European Working Group for Legionella Infection, Rome, Italy. ISTISAN Congressi 05/C2. Abstract book, p. 32.
12. FRY N.K., ALEXIOU-DANIEL S., BANGSBORG J.M., BERNANDER S., CASTELLANI- PASTORIS M., ETIENNE J., FORSBLOM B., GAIA V., HELBIG J.H., LINDSAY D.S., LÜCK P.C., PELAZ C., ULDUM S., HARRISON T.G. (1999). A multicenter evaluation of genotypic methods for the epidemiological typing of Legionella pneumophila serogroup 1: results of a pan-European study. Clin. Microbiol. Infect. 5, 462-477.
13. FRY N.K., BANGSBORG J.M., BERGMANS A., BERNANDER S., ETIENNE J., FRANZIN L., GAIA V., HASENBERGER P., BALADRON JIMENEZ B., JONAS D., LINDSAY D.S., MENTULA S., PAPOUTSI A., STRUELENS M., ULDUM S.A., VISCA P., WANNET W., HARRISON T.G. (2002). Designation of the European Working Group on Legionella Infections (EWGLI) amplified fragment length polymorphism types of Legionella pneumophila serogroup 1 and results of intercentre proficiency testing using a standard protocol. Eur. J. Clin. Microbiol. Infect. Dis. 21, 722-728.
14. FRY N.K., BANGSBORG J.M., BERNANDER S., ETIENNE J., FORSBLOM B., GAIA V., HASENBERGER P., LINDSAY D.S., PAPOUTSI A., PELAZ C., STRUELENS M., ULDUM S.A., VISCA P., HARRISON T.G. (2000). Assessment of intercentre reproducibility and epidemiological concordance of Legionella pneumophila serogroup 1 genotyping by amplified fragment length polymorphism analysis. Eur. J. Clin. Microbiol. Infect. Dis. 19, 773-780.
15. GAIA V, FRY N.K., AFSHAR B., LÜCK P.C., MEUGNIER H., ETIENNE J., PEDUZZI R., HARRISON T.G. (2005). Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila. J. Clin. Microbiol. 43, 2047-52.
16. GAIA V., FRY N.K., HARRISON G., PEDUZZI R. (2003). Sequence-based typing of Legionella pneumophila serogroup 1 offers the potential for true portability in legionellosis outbreak investigation. J. Clin. Microbiol 41, 2932-2939.
17. GARCIA M.T., JONES S., PELAZ C., MILLAR R.D., ABU KWAIK Y. (2007). Acanthamoeba polyphaga resuscitates viabe non-culturable Legionella pneumophila after disinfection. Environ. Micorbiol. 9, 1267-1277.
18. GREUB G., RAUOLT D. (2004). Microorganisms resistant to free-living amoebae. Clin. Microbiol. Rev. 17, 413-433.
19. JOSEPH C.A. (2002). Surveillance of Legionnaires' disease in Europe, p. 311-7. In: R. Marre, Y. Abu Kwaik, C. Bartlett, N. P. Cianciotto, B.S. Fields, M. Frosch, J. Hacker and P.C. Luck (ed.), Legionella. ASM Press, Washington, D.C.
20. LE FLÈCHE P., FABRE M., DENOEUD F., KOECK J.L., VERGNAUD G. (2002). High resolution, on-line identification of strains from Mycobacterium tuberculosis complex based on tandem repeat typing. BMC Microbiol. 2, 37.
21. LEONI E., DE LUCA G., LEGNANT P.P., SACCHETTI R., STAMPI S., ZANETTI F. (2005). Legionella waterline colonization: detection of Legionella species in domestic, hotel and hospital hot water systems. J. Appl. Microbiol. 98, 373-379.
22. RATZOW S., GAIA V., HELBIG J.H., FRY N.K., LÜCK P.C. (2007). Addition of neuA, the gene encoding N-acyl-neuraminate cytydyl transferase, increases the discriminatory ability of the concensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains. J. Clin. Microbiol. 45, 1965-1968.
23. RODGERS F.G. (1998). Legionella, p. 1147-65. In: L. Collier, A. Balows and M. Sussman (ed.), Topley & Wilson's Microbiology and Microbial Infections, vol. 2. Arnold, London.
24. SCATURRO M., LOSARDO M., DE PONTE G., RICCI M.L. (2005). Comparison of three molecular methods used for subtyping of Legionella pneumophila strains isolated during an epidemic of legionellosis in Rome. J. Clin. Microbiol. 43, 5348-5350.
25. STEINERT M., HENTSCHEL U., HACKER J. (2002). Legionella pneumophila: an aquatic microbe goes astray. Microbiol. Rev. 26, 149-162.
26. STOUT J.E., YU V.L., MURACA P. (1985). Isolation of Legionella pneumophila from the cold water of hospital ice machines: implications for its origin and transmission. Infect. Control. 6, 141-146.
27. STOUT J.E., YU V.L. (2004). Nosocomial Legionella infection, p. 603-21. In: C.G. Mayhall (ed.) Hospital Epidemiology and Infection Control. Lippincott Williams & Wilkins, Philadelphia, USA.
28. TERCELJ-ZORMAN M., SELJAK M., STARE J., MENCINGER J., RAKOVEC J., RYLANDER R., STRLE F. (2004). A hospital outbreak of Legionella from a contaminated water supply. Arch. Environ. Health. 59, 156-159.
29. WITHERELL L.E., DUNCAN R.W., STONE K.M., STRATTON L.J., ORCIARI L., KAPPEL S., JILLSON D.A. (1988). Investigation of L.pneumophila in drinking water. J. Am. Water Works Assoc. 80, 87-93.
30. WONG S., PABBARAJU K., BURK V.F., BROUKHANSKI G.C., FOX J., LOUIE T., MAH M.W., BERNARD K., TILLEY P.A. (2006). Use of sequence-based typing for investigation of a case of nosocomial legionellosis. J. Med. Microbiol. 55, 1707-1710.
31. ZANETTI F., STAMPI S., DE LUCAG., FATEH-MOGHADAM P, BUCCI SABATTINI M.A., CHECCHI L. (2000). Water characteristics associated with the occurrence of Legionella pneumophila in dental units. Eur. J. Oral Sci. 108, 22-28.