Estimating glutathione synthesis with deuterated water: A model for peptide biosynthesis

Analytical Biochemistry

Volumn 379, Issue 1, 2008, Pages 40-44

  Cabral, Carolina B ^a   Bullock, Kevin H ^b   Bischoff, David J ^b   Tompkins, Ronald G ^a,b   Yu, Yong M ^a,b   Kelleher, Joanne K ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b SHRINERS HOSPITALS FOR CHILDREN   (United States)

Author keywords

Deuterated water; Glutathione; Isotopomers; LC MS; Mass spectrometry; Models

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; DEUTERIUM; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MODELS;

ACID PRECURSORS; DEUTERATED WATER; GLUTATHIONES; ISOTOPOMERS; LC/MS; LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY; ORAL ADMINISTRATION; PEPTIDE BIOSYNTHESIS;

PEPTIDES;

AMINO ACID; CARBON; DEUTERIUM OXIDE; GLUTATHIONE; GLUTATHIONE N ETHYLMALEIMIDE; HYDROGEN; N ETHYLMALEIMIDE; TRIPEPTIDE;

ANIMAL CELL; ARTICLE; GLUTATHIONE METABOLISM; HYDROGEN BOND; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; RABBIT;

ORYCTOLAGUS CUNICULUS;

EID: 46549087761     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.04.042     Document Type: Article

References (18)

1. Han D., Hanawa N., Saberi B., and Kaplowitz N. Mechanisms of liver injury: III. Role of glutathione redox status in liver injury. Am. J. Physiol. Gastrointest. Liver Physiol. 291 (2006) G1-G7
2. Waelsch H., and Rittenberg D. The metabolism of glutathione. Science 90 (1939) 423-424
3. Sekura R., and Meister A. Glutathione turnover in the kidney: Considerations relating to the γ-glutamyl cycle and the transport of amino acids. Proc. Natl. Acad. Sci. USA 71 (1974) 2969-2972
4. Griffith O.W., and Meister A. Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S - n -butyl homocysteine sulfoximine). J. Biol. Chem. 254 (1979) 7558-7560
5. Reid M., and Jahoor F. Methods for measuring glutathione concentration and rate of synthesis. Curr. Opin. Clin. Nutr. Metab. Care 3 (2000) 385-390
6. 2 O: Application to patients with end-stage renal disease. Am. J. Physiol. Endocrinol. Metab. 286 (2004) E665-E672
7. Busch R., Kim Y.K., Neese R.A., Schade-Serin V., Collins M., Awada M., Gardner J.L., Beysen C., Marino M.E., Misell L.M., and Hellerstein M.K. Measurement of protein turnover rates by heavy water labeling of nonessential amino acids. Biochim. Biophys. Acta 1760 (2006) 730-744
8. 2 O) and mass isotopomer analysis. Am. J. Physiol. 266 (1994) E372-E383
9. Hu R.H., Yu Y.M., Costa D., Young V.R., Ryan C.M., Burke J.F., and Tompkins R.G. A rabbit model for metabolic studies after burn injury. J. Surg. Res. 75 (1998) 153-160
10. Steghens J.P., Flourie F., Arab K., and Collombel C. Fast liquid chromatography-mass spectrometry glutathione measurement in whole blood: Micromolar GSSG is a sample preparation artifact. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 798 (2003) 343-349
11. 3 ]acetone and gas chromatography-mass spectrometry. Anal. Biochem. 258 (1998) 315-321
12. Jahoor F., Wykes L.J., Reeds P.J., Henry J.F., del Rosario M.P., and Frazer M.E. Protein-deficient pigs cannot maintain reduced glutathione homeostasis when subjected to the stress of inflammation. J. Nutr. 125 (1995) 1462-1472
13. Reid M., Badaloo A., Forrester T., Morlese J.F., Frazer M., Heird W.C., and Jahoor F. In vivo rates of erythrocyte glutathione synthesis in children with severe protein-energy malnutrition. Am. J. Physiol. Endocrinol. Metab. 278 (2000) E405-E412
14. Lyons J., Rauh-Pfeiffer A., Yu Y.M., Lu X.M., Zurakowski D., Tompkins R.G., Ajami A.M., Young V.R., and Castillo L. Blood glutathione synthesis rates in healthy adults receiving a sulfur amino acid-free diet. Proc. Natl. Acad. Sci. USA 97 (2000) 5071-5076
15. 15N]glutamic acid and homoglutathione to determine both glutathione synthesis and concentration by gas chromatography-mass spectrometry (GCMS). J. Mass Spectrom. 36 (2001) 726-735
16. 14 -glycine. J. Biol. Chem. 222 (1956) 581-585
17. Ookhtens M., and Kaplowitz N. Role of the liver in interorgan homeostasis of glutathione and cyst(e)ine. Semin. Liver Dis. 18 (1998) 313-329
18. Wu G., Fang Y.Z., Yang S., Lupton J.R., and Turner N.D. Glutathione metabolism and its implications for health. J. Nutr. 134 (2004) 489-492