Digital differential interference contrast autofocus for high-resolution oil-immersion microscopy

Cytometry Part A

Volumn 73, Issue 7, 2008, Pages 658-666

  Shen, Feimo ^a   Hodgson, Louis ^a   Price, Jeffrey H ^b,c   Hahn, Klaus M ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b BURNHAM INSTITUTE   (United States)

^c VALA SCIENCES INC   (United States)

Author keywords

Autofocus; Differential interference contrast; Image cytometry; Lab automation; Modulation transfer function

Indexed keywords

CELL PROTEIN;

ACCURACY; ANALYZER; ARTICLE; AUTOMATION; BIOSENSOR; CELL GROWTH; DIFFERENTIAL INTERFERENCE CONTRAST MICROSCOPY; FLUORESCENCE; MICROSCOPY; OIL IMMERSION MICROSCOPY; PHASE CONTRAST MICROSCOPY; PHOTOTOXICITY; PRIORITY JOURNAL;

ALGORITHMS; BIOSENSING TECHNIQUES; COMPUTERS; EQUIPMENT DESIGN; FILTRATION; FLUORESCENT DYES; IMAGE CYTOMETRY; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY; MICROSCOPY, INTERFERENCE; MICROSCOPY, PHASE-CONTRAST; MODELS, STATISTICAL; OILS; SOFTWARE;

EID: 46449138336     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20558     Document Type: Article

References (16)

1. Shen F, Hodgson L, Rabinovich A, Pertz O, Hahn K, Price JH. Functional proteometrics for cell migration. Cytometry Part A 2006;69A:563-572.
2. Gaits F, Hahn K. Shedding light on cell signaling: Interpretation of FRET biosensors. Sci STKE 2003;165:3.
3. Pertz O, Hodgson L, Klemke R, Hahn KM. Spatio-temporal dynamics of RhoA activity in migrating cells. Nature 2006;440:1069-1072.
4. Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM. Activation of endogenous Cdc42 visualized in living cells. Science 2004;305:1615-1619.
5. Lalonde S, Ehrhardt DW, Frommer WB. Shining light on signaling and metabolic networks by genetically encoded biosensors. Curr Opin Plant Biol 2005;6:574-581.
6. Inoue S, Spring KR. Video Microscopy, The Fundamentals, 2nd ed. New York: Plenum; 1997.584 p.
7. Ingram M, Preston K Jr. Automated analysis of blood cells. Sci Am 1970;223:72-82.
8. Mason DC, Green DK. Automatic focusing of a computer controlled microscope. IEEE Trans Biomed Eng BME 1975;22:312-317.
9. Jarvis RA. Focus optimization criteria for computer image processing. Microscope 1976;24:163-171.
10. Groen FC, Young IT, Ligthart G. A comparison of different focus functions for use in autofocus algorithms. Cytometry 1985;6:81-91.
11. Price JH, Cough DA. Comparison of phase-contrast and fluorescence digital autofocus for scanning microscopy. Cytometry 1994;16:283-297.
12. Oliva MA, Bravo-Zanoguera M, Price J. Filtering out contrast reversals for microscopy autofocus. Appl Opt 1999;38:638-646.
13. Bravo-Zanoguera M, Massenbach B, Kellner A, Price J. High-performance autofocus circuit for biological microscopy. Rev Sci Instrum 1998;69:3966-3977.
14. Lanni F, Waggoner A, Taylor D. Internal reflection fluorescence microscopy. J Cell Biol 1985;100:1091.
15. Born, M, Wolf. E. Principles of Optics, 6th ed. Oxford: Pergamon; 1980.
16. Beuthan J, Minet O, Helfman J, Muller G. The spatial variation of the refractive index in biological cells. Phys Med Biol 1996;41:369-382.