Single-chain antibody against human lipocalin-type prostaglandin D synthase: Construction, expression, purification, and activity assay

Biochemistry (Moscow)

Volumn 73, Issue 6, 2008, Pages 702-710

  Chen, De Yu ^a,b   Liu, Li Min ^a   Liu, Sheng Jie ^b   Zhu, Mao Ying ^b   Xu, Lan ^a   Huang, Tian Hua ^a  

^a SHANTOU UNIVERSITY MEDICAL COLLEGE   (China)

^b FUYANG NORMAL UNIVERSITY   (China)

Author keywords

Expression; Prostaglandin D synthase (L PGDS); Single chain antibody (ScFv)

Indexed keywords

CLONING; ESCHERICHIA COLI; LIPIDS; MONOCLONAL ANTIBODIES; PURIFICATION;

EXPRESSION; INCLUSION BODIES; MURINE MONOCLONAL ANTIBODY; NITRILOTRIACETIC ACID; PROSTAGLANDIN D SYNTHASE; SINGLE-CHAIN ANTIBODIES; SPECIFIC RECOGNITION; VARIABLE REGIONS;

POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI; MURINAE;

IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; ISOMERASE; LIPOCALIN; PROSTAGLANDIN R2 D ISOMERASE; PROSTAGLANDIN R2 D-ISOMERASE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOLOGICAL MODEL; BIOSYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; PROTEIN ENGINEERING; TUMOR CELL LINE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL LINE, TUMOR; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; INTRAMOLECULAR OXIDOREDUCTASES; LIPOCALINS; MICE; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING;

EID: 46449133370     PISSN: 00062979     EISSN: 16083040     Source Type: Journal    
DOI: 10.1134/S0006297908060114     Document Type: Article

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