Selective cytotoxicity of scFv-TNF fusion protein on the tumor cells

Biotechnology Letters

Volumn 24, Issue 6, 2002, Pages 441-443

  Luo, Dong ^a   Li, Hongshi ^b   Tang, Xiao Bo ^c  

^a UNIVERSITY OF ALBERTA   (Canada)

^b CanaTech   (Canada)

^c UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

Antibody engineering; Cytotoxicity; ScFv; TNF

Indexed keywords

CA 125 ANTIGEN; HYBRID PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TUMOR NECROSIS FACTOR ALPHA;

ANTIBODY ENGINEERING; ARTICLE; CELL KILLING; CONTROLLED STUDY; CYTOTOXICITY; HUMAN; HUMAN CELL; IC 50; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; TUMOR CELL LINE;

PICHIA; PICHIA PASTORIS;

EID: 0036204215     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1014596924446     Document Type: Article

References (9)

1. Phase I clinical trial of recombinant human tumor necrosis factor
2. Tumor necrosis factor can induce both apoptic and necrotic forms of cell lysis
3. Expression of a fusion protein of scFv-biotin mimetic peptide for immunoassay
4. Vl-linker-Vh orientation-dependent expression of single chain Fv-containing an engineered disulfide-stabilized bond in the framework regions
5. High level secretion of single-chain antibody in Pichia expression system
6. Construction, binding properties, metabolism and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49
7. Recombinant toxins for cancer treatment
8. Human turnout necrosis factor: Precursor structure, expression and homology to lymphotoxin
9. Rapid tumor penetration of single-chain Fv and comparision with other immunoglobulin forms