Reversible 5′-end biotinylation and affinity purification of synthetic RNA

Tetrahedron Letters

Volumn 45, Issue 43, 2004, Pages 7987-7990

  Fang, Shiyue ^a   Bergstrom, Donald E ^a  

^a NONE

Author keywords

Affinity purification; Avidin; Diisopropylsilylacetal linker; Oligoribonucleotide; Phosphoramidite; Reversible biotinylation; RNA; Solid phase synthesis

Indexed keywords

BUFFER; FLUORIDE; MICROSPHERE; OLIGOMER; OLIGORIBONUCLEOTIDE; PHOSPHORAMIDOUS ACID; PHOSPHORUS DERIVATIVE; RNA; TETRABUTYLAMMONIUM FLUORIDE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BIOTINYLATION; DEPROTECTION REACTION; PURIFICATION; QUALITY CONTROL; SOLID; SYNTHESIS;

EID: 4644370975     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tetlet.2004.09.019     Document Type: Article

References (20)

1. J.L. McInnes, and R.H. Symons Nucleic Acid Probes R.H. Symons Preparation and Detection of Nonradioactive Nucleic Acid and Oligonucleotide Probes 1989 CRC Boca Raton, FL 33 80
2. P.R. Langer, A.A. Waldrop, and D.C. Ward Proc. Natl. Acad. Sci. U.S.A. 78 1981 6633 6637
3. M.S. Urdea, B.D. Warner, J.A. Running, M. Stempien, J. Clyne, and T. Horn Nucleic Acids Res. 16 1988 4937 4956
4. B.D. Gildea, J.M. Coull, and H. Koster Tetrahedron Lett. 31 1990 7095 7098
5. J. Olejnik, E. Krzymanska-Olejnik, and K.J. Rothschild Nucleic Acids Res. 24 1996 361 366
6. M. Shimkus, J. Levy, and T. Herman Proc. Natl. Acad. Sci. U.S.A. 82 1985 2593 2597
7. M.M. Greenberg Tetrahedron 51 1995 29 38
8. M.M. Greenberg, and J.L. Gilmore J. Org. Chem. 59 1994 746 753
9. J. Cadet, and P. Vigny Bioorganic Photochemistry H. Morrison The Photochemistry of Nucleic Acids Vol. 1 1990 John Wiley & Sons New York, NY 170 184
10. S. Fang, and D.E. Bergstrom Nucleic Acids Res. 31 2003 708 715
11. S. Fang, and D.E. Bergstrom Bioconjugate Chem. 14 2003 80 85
12. 2′-O-ACE-uridine 3 was purchased from Dharmacon, Inc
13. 19SSi 1221.56, found 1221.35
14. 3, 300 MHz) δ 168.30, 168.57
15. S.A. Scaringe, F.E. Wincott, and M.H. Caruthers J. Am. Chem. Soc. 120 1998 11820 11821
16. B.H. Dahl, K. Bjergarde, L. Henriksen, and O. Dahl Acta Chem. Scand. 44 1990 639 641
17. 4, adjusted to pH 7.0 with HCl
18. NAP™-25 column was purchased from Amersham Biosciences. Desalting was performed following manufacture-suggested procedure
19. The gel was imaged on a Molecular Dynamics Storm (860) system after staining in a SYBR® Gold (purchased from Molecular Probes, Inc.) solution in 1x TBE buffer (1:10,000) at rt for 30 min
20. RNAs 6 and 7 were identified with MALDI mass spectra. Biotinyl RNA 6: calcd 5287, found 5289. Unmodified RNA 7: calcd 4687, found 4687