Reversible biotinylation phosphoramidite for 5′-end-labeling, phosphorylation, and affinity purification of synthetic oligonucleotides

Bioconjugate Chemistry

Volumn 14, Issue 1, 2003, Pages 80-85

  Fang, Shiyue ^a,b   Bergstrom, Donald E ^a,b  

^a PURDUE UNIVERSITY   (United States)

^b WALTHER CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COENZYMES; DNA SEQUENCES; FLUORINE COMPOUNDS; MICROSPHERES; MIXTURES; OLIGONUCLEOTIDES; PURIFICATION; SYNTHESIS (CHEMICAL);

5′-END LABELING; AFFINITY PURIFICATION; BIOTINYLATIONS; BIS(HYDROXYMETHYL); HYDROXYL GROUPS; MALONATES; PHOSPHORAMIDITES; SOLID PHASIS; SOLID-PHASE; SYNTHESISED;

DNA;

ACETAL; AMMONIA; BIOTIN; BUFFER; DIETHYL BIS(HYDROXYMETHYL)MALONATE; DNA; HYDROXYL GROUP; MALONIC ACID DERIVATIVE; MICROSPHERE; OLIGONUCLEOTIDE; ORGANOPHOSPHATE; PHOSPHORAMIDIC ACID DERIVATIVE; PYRIDINE; UNCLASSIFIED DRUG; WATER;

ARTICLE; BIOTINYLATION; DNA CLEAVAGE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHOSPHORYLATION; PURIFICATION; SOLID; SYNTHESIS;

EID: 0037260948     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc025626o     Document Type: Article

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