Purification and characterization of human uroporphyrinogen III synthase expressed in Escherichia coli

Journal of Biochemistry

Volumn 136, Issue 2, 2004, Pages 211-220

  Omata, Yoshiaki ^a   Sakamoto, Hiroshi ^a   Higashimoto, Yuichiro ^a   Hayashi, Shunsuke ^a   Noguchi, Masato ^a  

^a KURUME UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Congenital erythropoietic porphyria (CEP); Hydroxymethylbilane synthase; Porphyrin synthesis; Uroporphyrinogen III synthase

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); CYSTEINE; DITHIOTHREITOL; EDETIC ACID; PHOSPHATE; PORPHOBILINOGEN DEAMINASE; PORPHYRIN; UROPORPHYRINOGEN III SYNTHASE;

ARTICLE; CELL LYSATE; CONGENITAL ERYTHROPOIETIC PORPHYRIA; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; HEME SYNTHESIS; NONHUMAN; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RESIDUE ANALYSIS; THERMOSTABILITY;

BIOCHEMISTRY; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; DITHIOTHREITOL; DNA, COMPLEMENTARY; EDETIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC VECTORS; GLYCEROL; HEAT; HUMANS; HYDROXYMETHYLBILANE SYNTHASE; KINETICS; MODELS, CHEMICAL; OXYGEN; PORPHYRINS; SULFHYDRYL COMPOUNDS; TEMPERATURE; TIME FACTORS; UROPORPHYRINOGEN III SYNTHETASE;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 4644362890     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh111     Document Type: Article

References (32)

1. Dailey, H.A. (1997) Enzymes of heme biosynthesis. J. Biol. Inorg. Chem. 2, 411-417
2. Romeo, G. and Levin, E.Y. (1969) Uroporphyrinogen III cosynthetase in human congenital erythropoietic porphyria. Proc. Natl Acad. Sci. USA 63, 856-863
3. Romeo, G., Glenn, B.L., and Levin, E.Y. (1970) Uroporphyrinogen III cosynthetase in asymptomatic carriers of congenital erythpoietic porphyria. Biochem. Genet. 4, 719-726
4. Chretien, S., Dubart, A., Beaupain, D., Raich, N., Grandchamp, B., Rosa, J., Goossens, M., and Romeo, P.H. (1988) Alternative transcription and splicing of the human porphobilinogen deaminase gene result either in tissue-specific or in housekeeping expression. Proc. Natl. Acad. Sci. USA 85, 6-10
5. Aizencang, G., Solis, C., Bishop, D.F., Warner, C., and Desnick, R.J. (2000) Human uroporphyrinogen-III synthase: genomic organization, alternative promoters, and erythroid-specific expression. Genomics 70, 223-231
6. Battersby, A.R. and Leeper, F.J. (1990) Biosynthesis of the pigments of life: mechanistic studies on the conversion of porphobilinogen to uroporphyrinogen III. Chem. Rev. 90, 1261-1274
7. Battersby, A.R., Hodgson, G.L., Hunt, E., McDonald, E., and Saunders, J. (1976) Biosynthesis of porphyrins and related macrocycles, Part VI, Nature of the rearrangement process leading to the natural type III porphyrins. J. Chem. Soc. Perkin I 273-282
8. Pichon, C., Atshaves, B.P., Stolowich, N.J., and Scott, A.I. (1994) Evidence for an intermediate in the enzymatic formation of uroporphyrinogen III. Bioorg. Med. Chem. 2, 153-168
9. Battersby, A.R., Fookes, C.J.R., McDonald, E., and Meegan, M.J. (1978) Biosynthesis of type-III porphyrins: proof of intact enzymic conversion of the head-to-tail bilane into uro'gen-III by intramolecular rearrangement. J. Chem. Soc. Chem. Commun. 185-186
10. Tietze, L.F. and Geissler, H. (1993) Investigations in the biosynthesis of the pigments of life: calculations on the formation of uroporphyrinogen III from hydroxymethylbilan and description of a new mechanism for the D-ring inversion. Angew. Chem. Int. Ed. Engl. 32, 1040-1042
11. Leeper, F.J. (1994) The evidence for a spirocyclic intermediate in the formation of uroporphyrinogen III by cosynthase. Ciba Found. Symp. 180, 111-130
12. Hart, G.J. and Battersby, A.R. (1985) Purification and properties of uroporphyrinogen III synthase (co-synthetase) from Euglena gracilis. Biochem. J. 232, 151-160
13. Tsai, S., Bishop, D.F., and Desnick, R.J. (1987) Purification and properties of uroporphyrinogen III synthase from human erythrocytes. J. Biol. Chem. 262, 1268-1273
14. Smythe, E. and Williams, D.C. (1988) Rat liver uroporphyrinogen III synthase has similar properties to the enzyme from Euglena gracilis, including absence of a requirement for a reversibly bound cofactor for activity. Biochem. J. 253, 275-279
15. Alwan, A.F., Mgbeje, B.I.A., and Jordan, P.M. (1989) Purification and properties of uroporphyrinogen III synthase (co-synthase) from an overproducing recombinant strain of Escherichia coli K-12. Biochem. J. 264, 397-402
16. Stamford, N.P.J., Capretta, A., and Battersby, A.R. (1995) Expression, purification and characterisation of the product from the Bacillus subtilis hemD gene, uroporphyrinogen III synthase. Eur. J. Biochem. 231, 236-241
17. Tsai, S., Bishop, D.F., and Desnick, R.J. (1988) Human uroporphyrinogen III synthase: molecular cloning, nucleotide sequence, and expression of a full-length cDNA. Proc. Natl Acad. Sci. USA 85, 7049-7053
18. Boulechfar, S., Silva, V.D., Deybach, J.-C., Nordmann, Y., Grandchamp, B., and Verneuil, H. (1992) Heterogeneity of mutations in the uroporphyrinogen III synthase gene in congenital erythropoietic porphyria. Hum. Genet. 88, 320-324
19. Mathews, M.A.A., Schubert, H.L., Whitby, F.G., Alexander, K.J., Schadick, K., Bergonia, H.A., Phillips, J.D., and Hill, C.P. (2001) Crystal structure of human uroporphyrinogen III synthase. EMBO J. 20, 5832-5839
20. Grandchamp, B., De Verneuil, H., Beaumont, C., Chretien, S., Walter, O., and Nordmann, Y. (1987) Tissue-specific expression of porphobilinogen deaminase. Two isoenzymes from a single gene. Eur. J. Biochem. 162, 105-110
21. Craig III, S.P., Yuan, L., Kuntz, D.A., McKerrow, J.H., and Wang, C.C. (1991) High level expression in Escherichia coli of soluble, enzymatically active schistosomal hypoxanthine/guanine phosphoribosyltransferase and trypanosomal ornithine decarboxylase. Proc. Natl Acad. Sci. USA 88, 2500-2504
22. Anderson, P.M. and Desnick, R.J. (1980) Purification and properties of uroporphyrinogen I synthase from human erythrocytes. Identification of stable enzyme-substrate intermediates. J. Biol. Chem. 255, 1993-1999
23. Anderson, P.M. and Desnick, R.J. (1982) Porphobilinogen deaminase: methods and principles of the enzymatic assay. Enzyme 28, 146-157
24. Rimington, C. (1960) Spectral-absorption coefficients of some porphyrins in the Soret-band region. Biochem. J. 75, 620-623
25. Jordan, P.M. (1982) Uroporphyrinogen III cosynthetase: a direct assay method. Enzyme 28, 158-169
26. Tsai, S., Bishop, D.F., and Desnick, R.J. (1987) Coupled-enzyme and direct assays for uroporphyrinogen III synthase activity in human erythrocytes and cultured lymphoblasts. Enzymatic diagnosis of heterozygotes and homozygotes with congenital erythropoietic porphyria. Anal. Biochem. 166, 120-133
27. Shoolingin-Jordan, P.M. and Leadbeater, R. (1997) Coupled assay for uroporphyrinogen III synthase. Methods Enzymol. 281, 327-336
28. Jordan, P.M., Burton, G., Nordlöv, H., Schneider, M.M., Pryde, L., and Scott, A.I. (1979) Pre-uroporphyrinogen: a substrate for uroporphyrinogen III cosynthase. J. Chem. Soc. Chem. Commun. 204-205
29. Riddles, P.W., Blakeley, R.L., and Zerner, B. (1983) Reassessment of Ellman's reagent. Methods Enzymol. 91, 49-60
30. Clement, R.P., Kohashi, M., and Piper, W.N. (1982) Rat hepatic uroporphyrinogen III cosynthase: purification, properties, and inhibition by metal ions. Arch. Biochem. Biophys. 214, 657-667
31. Kohashi, M., Clement, R.P., Tse, J., and Piper, W.N. (1984) Rat hepatic uroporphyrinogen III co-synthase. Purification and evidence for a bound folate coenzyme participating in the biosynthesis of uroporphyrinogen III. Biochem. J. 220, 755-765
32. Desnick, R.J., Glass, I.A., Xu, W., Solis, C., and Astrin, K.H. (1998) Molecular genetics of congenital erythropoietic porphyria. Semin. Liver Dis. 18, 77-84