Development and transfection of short-term primary cell cultures from Brugia malayi

Molecular and Biochemical Parasitology

Volumn 137, Issue 2, 2004, Pages 345-348

  Higazi, Tarig B ^a   Shu, Limin ^a   Unnasch, Thomas R ^a  

^a UNIVERSITY OF ALABAMA SCHOOL OF MEDICINE   (United States)

Author keywords

5 bromo 2 deoxyuridine; BrdU; Cell culture; FBS; fetal bovine serum; Filaria; Filariasis; GFP; green florescent protein; Nematode; PBS; phosphate buffered saline; simian virus large T antigen; SV40LT; Transfection

Indexed keywords

BROXURIDINE; CHITINASE; GREEN FLUORESCENT PROTEIN; HEAT SHOCK PROTEIN 70; PRONASE;

ANIMAL CELL; ARTICLE; BIOLISTIC TRANSFORMATION; BRUGIA MALAYI; CAENORHABDITIS ELEGANS; CELL CULTURE; CELL GROWTH; CELL IMMORTALIZATION; CELL METABOLISM; CELL PROLIFERATION; CELL SHAPE; CELL SIZE; CELL STRUCTURE; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; EGG SHELL; EMBRYO; EMBRYO DEVELOPMENT; FEMALE; FILARIA; GENE EXPRESSION; GENETIC TRANSFECTION; METHODOLOGY; NONHUMAN; PARASITE DEVELOPMENT; PRIORITY JOURNAL; PROMOTER REGION; PURIFICATION; REPORTER GENE; SEDIMENTATION; SERUM; TISSUE CULTURE;

ANIMALS; BRUGIA MALAYI; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL SURVIVAL; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; RECOMBINANT PROTEINS; TRANSFECTION;

ANIMALIA; BOVINAE; BRUGIA; BRUGIA MALAYI; CAENORHABDITIS; CAENORHABDITIS ELEGANS; FILARIA; SIMIAE;

EID: 4644320148     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2004.06.004     Document Type: Article

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