Production in vitro of toxic macromolecules by strains of Beauveria bassiana, and purification of a chitosanase-like protein secreted by a melanizing isolate

Comparative Biochemistry and Physiology - C Toxicology and Pharmacology

Volumn 138, Issue 2, 2004, Pages 149-161

  Fuguet, Rosa ^a   Théraud, Magali ^a   Vey, Alain ^a  

^a France   (France)

Author keywords

Beauveria bassiana; Chitosanase; Cytotoxicity; Entomopathogenic fungus; Galleria mellonella; Insect; Melanization; Micro sequencing; Purification; Toxic protein

Indexed keywords

2 DIETHYLAMINOETHANOL; CHITOSANASE; FUNGAL PROTEIN; PROTEIN BCLP; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BEAUVERIA; CHROMATOGRAPHY; CONTROLLED STUDY; CYTOTOXICITY; DIALYSATE; ELECTROPHORESIS; ELUTION; ENZYME PURIFICATION; FUNGAL STRAIN; FUSARIUM SOLANI; HYDROPHILICITY; INSECTICIDAL ACTIVITY; LEPIDOPTERA; MOLECULAR WEIGHT; MYCOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; SEPARATION TECHNIQUE; SEQUENCE HOMOLOGY;

BEAUVERIA; BEAUVERIA BASSIANA; CORDYCEPS BASSIANA; FUNGI; FUSARIUM; FUSARIUM SOLANI; GALLERIA MELLONELLA; INSECTA; LEPIDOPTERA;

EID: 4644318648     PISSN: 15320456     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2004.06.009     Document Type: Article

References (38)

1. Anonymous Statistics for Windows Analytical Software 1996
2. M.M. Bradford A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
3. M. Brehélin Hemolymph coagulation in Locusta migratoria: evidence for a functional equivalent of fibrinogen Comp. Biochem. Physiol. 62 1979 329 334
4. M. Brehélin Role of haemocytes in hemolymph coagulation in L. migratoria Experientia 35 1979 270
5. M. Brehélin, and D. Zachary Insect haemocytes: a new classification to rule out the controversy M. Brehélin Immunity in Invertebrates 1986 Springer-Verlag Berlin 36 48
6. A.K. Charnley Mechanisms of fungal pathogenesis in insects J.M. Whipps R.D. Lumsden Biotechnology of Fungi for Improving Plant Growth 1989 Cambridge Univ. Press Sydney 85 116
7. A.K. Charnley, and R.J. St. Leger The role of cuticle degrading enzymes in fungal pathogenesis in insects G.T. Cole H.C. Hoch The Fungal Spore and Disease Initiation in Plant and Animals 1991 Plenum New York 267 286
8. C.Y. Cheng, and Y.K. Li An Aspergillus chitosanase with potential for large-scale preparation of chitosan oligosacharides Biotechnol. Appl. Biochem. 32 2000 197 203
9. C. Dumas, V. Matha, C. Quiot, V. Matha, J.-M. Quiot, and A. Vey Effect of destruxins, cyclic depsipeptide mycotoxins, on calcium balance and phosphorylation of intracellular proteins in lepidopteran cell lines Comp. Biochem. Physiol. C114 1996 213 219
10. R. Fuguet, and A. Vey Comparative analysis of the production of insecticidal and melanizing molecules by strains of Beauveria spp.: in vivo studies J. Invertebr. Pathol. 85 2004 152 167
11. J. Griesch, and A. Vilcinskas Proteases released by entomopathogenic fungi impair phagocytic activity, attachment and spreading of plasmatocytes isolated from the haemolymph of the greater wax moth Galleria mellonella Biocontrol Sci. Technol. 8 1998 517 531
12. B.D. Hames One dimensional polyacrylamide gel electrophoresis B.D. Hames D. Rickwood Gel Electrophoresis of Proteins: a practical approach 1990 IRL Press Oxford, UK 1 147
13. B. Kaplan, and M. Pras Separation of proteins by consecutive sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and reverse phase high performance liquid chromatography Biomed. Chromatogr. 5 1991 86 89
14. G.G. Khachatourians Biochemistry and molecular biology of entomopathogenic fungi D.E. Howard J.D. Miller The Mycota: VI. Human and Animal Relationships 1996 Springer Verlag Berlinâ€"Heidelberg 331 363
15. M. Kucera, and A. Samsinakova Toxins of entomophagous fungus Beauveria bassiana J. Invertebr. Pathol. 12 1968 316 320
16. J. Kurt, and W. Stoffel A facile method for the isolation and preparation of proteins and peptides for sequence analysis in the picomolar range Biol.Chem. Hoppe-Seyler 371 1990 675 685
17. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
18. I. Mazet, S.-Y. Hung, and D.G. Boucias Detection of toxic metabolites in the hemolymph of Beauveria bassiana infected Spodoptera exigua larvae Experientia 50 1994 142 147
19. P. Mollier, J. Lagniel, B. Fournet, A. Aïoun, and G. Riba A glycoprotein highly toxic for Galleria mellonella larvae secreted by the entomopathogenic fungus Beauveria sulfurescens J. Invertebr. Pathol. 64 1994 200 207
20. E. Müller-Kögler On mass cultivation, determination of effectiveness, and standardization of insect pathogenic fungi P.A. Van der Laan Proc. Intern. Colloq. 1967 Insect Pathology and Microbial Control Wageningen North-Holland, Amsterdam 339 353
21. E. Quesada-Moraga, and A. Vey Intra-specific variation in virulence and in vitro production of macromolecular toxins active against locust among Beauveria bassiana strains, and effects of in vivo and in vitro passages on these factors Biocontrol Sci. Technol. 13 2003 323 340
22. E. Quesada-Moraga, and A. Vey Bassiacridin, a protein toxic for locusts secreted by the enthomopathogenic fungus Beauveria bassiana Mycol. Res. 108 4 2004 441 452
23. D.W. Roberts Toxins of entomopathogenic fungi H.D. Burges Microbial Control of Pests and Plant Diseases 1970â€"1980 1981 Academic Press New York 441 464
24. J. Rosenfeld, J. Capdevielle, J.C. Guillemot, and P. Ferrara In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis Anal. Biochem. 203 1992 173 179
25. R.J. Saint-Léger Biology and mechanisms of insect cuticle invasion by deuteromycete fungal pathogens N.E. Beckage S.N. Thompson B.A. Frederici Parasites and Pathogens of Insects vol. II 1993 Academic Press New York 211 229
26. M. Schumacher, M.O. Glocker, M. Wunderlin, and M. Przybylski Direct isolation of proteins from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analysis by electrospray-ionization mass spectrometry Electrophoresis 17 1996 848 854
27. S. Shimizu, Y. Tsuchitani, and T. Matsumoto Production of an extracellular protease by Beauveria bassiana in the haemolymph of the silkworm, Bombyx mori Lett. Appl. Microbiol. 16 1993 291 294
28. S. Shimizu, Y. Tsuchitani, and T. Matsumoto Serology and substrate-specificity of extracellular proteases from 4 species of entomopathogenic hyphomycetes J. Invertebr. Pathol. 61 1993 192 195
29. M. Shimozaka, M. Kumehara, X.Y. Zhang, M. Nogawa, and M. Okazaki Cloning and characterization of a chitosanase gene from the plant pathogenic fungus Fusarium solani J. Ferment. Bioeng. 82 1996 426 431
30. M. Shimozaka, K. Kuroiwa, and M. Okazaki Cloning of a chitosanase gene from the fungus Fusarium solani 2002 GenBank accession numbers: AB090326 ; BAC10609. EMBL/GenBank/DDBJ databases
31. SPSS, 1997. SPSS 8.0 for Windows. SPSS Inc. Headquarters, 233 S. Wacker Drive, 11th floor, Chicago, IL. 60606, USA.
32. H. Strasser, A. Vey, and T. Butt Are there any risks in using enthomopathogenic fungi for pest control, in particular reference to the bioactive metabolites of Metarhizium, Tolypocladium and Beauveria species? Biocontrol Sci. Technol. 10 2000 717 735
33. A. Vey, J.-M. Quiot, I. Mazet, and C.W. McCoy Toxicity and pathology of crude broth filtrate produced by Hirsutella thompsonii var. thompsonii in shake culture J. Invertebr. Pathol. 61 1993 131 137
34. A. Vey, R. Hoagland, and T.M. Butt Toxic metabolites of fungal biocontrol agents T.M. Butt C.W. Jackson N. Magan Fungi as Biocontrol Agents: Progress, Problems and Potential 2001 CABI Publishing 311 346
35. A. Vey, V. Matha, and C. Dumas Effect of the peptide mycotoxin destruxin E on insect haemocytes and on dynamics and efficiency of the multicellular immune reaction J. Invertebr. Pathol. 80 2002 177 187
36. A. Vilcinskas, A. Jegorov, Z. Landa, P. Götz, and V. Matha Effects of beauverolide L and cyclosporin A on humoral and cellular immune response of the greater wax moth, Galleria mellonella Comp. Biochem. Physiol. C122 1999 83 92
37. J. Weiser, V. Matha, Z. Zizka, and A. Jegorov Pathology of cyclosporin A in mosquito larvae Cytobios 59 1989 143 150
38. J. Weiser, and V. Matha The insecticidal activity of cyclosporines on mosquito larvae J. Invertebr. Pathol. 51 1988 92 93