Direct isolation of proteins from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analysis by electrospray-ionization mass spectrometry

Electrophoresis

Volumn 17, Issue 5, 1996, Pages 848-854

  Schuhmacher, Martina ^a   Glocker, Michael O ^a   Wunderlin, Markus ^a   Przybylski, Michael ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

Covalent protein adducts; Electroelution; Electrospray ionization mass spectrometry; Protein mixtures; Sodium dodecyl sulfate polyacrylamide gel electrophoresis

Indexed keywords

BODY FLUIDS; ELECTRODEPOSITION; ELECTROPHORESIS; ELECTROSPRAY IONIZATION; MASS SPECTROMETRY; MOLECULAR WEIGHT; NEGATIVE IONS; REDUCING AGENTS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

BUFFER SYSTEM; COVALENT PROTEIN ADDUCT; ELECTROELUTION; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; GEL ANALYSIS; MOLECULAR IONS; MOLECULAR WEIGHT DETERMINATION; PROTEIN ADDUCTS; PROTEIN MIXTURES; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;

PROTEINS;

BOVINE SERUM ALBUMIN; INSULIN; LYSOZYME; MYOGLOBIN; PLASMA PROTEIN;

CONFERENCE PAPER; CONTROLLED STUDY; DOG; GEL ELECTROPHORESIS; HEMOFILTRATION; HORSE; HUMAN; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SHEEP; TECHNIQUE; UREMIA;

EID: 0030051007     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150170506     Document Type: Conference Paper

References (22)

1. Patterson, S. D., Aebersold, R., Electrophoresis 1995, 16, 1791-1814.
2. Dianoux, A. C., Stasia, M. J., Garin, J., Gagnon, J., Vignais, P. V., Biochemistry 1992, 31, 5898-5905.
3. Biemann, K., Martin, S. A., Mass Spectrom. 1987, 6, 1-75.
4. Burlingame, A. L., Baillie, T. A., Russel, D. H., Anal. Chem. 1992, 64, 467R-502R.
5. Smith, R. D., Loo, J. A., Orgorzalek-Loo, R. R., Busman, M., Udseth, H. R., Mass Spectrom. Rev. 1991, 10, 359-452.
6. Karas, M., Bahr, U., Gießmann, U., Mass Spectrom. Rev. 1991, 10, 335-357.
7. Loo, R. R. O., Dales, N., Andrews, P. C., Protein Sci. 1994, 3, 1975-1983.
8. Eckerskorn, C., Strupat, K., Karas, M., Hillenkamp, F., Lottspeich, F., Electrophoresis 1992, 13, 664-665.
9. Strupat, K., Karas, M., Hillenkamp, F., Anal. Chem. 1994, 66, 464-470.
10. Vestling, M. M., Fenselau, C., Anal. Chem. 1994, 66, 471-477.
11. Rasmussen, H. H., Mørtz, E., Mann, M., Roepstorff, P., Celis, J. E., Electrophoresis 1994, 15, 406-416.
12. Mørtz, E., Vorm, O., Mann, M., Roepstorff, P., Biol. Mass Spectrom. 1994, 23, 249-261.
13. Haebel, S., Jensen, C., Andersen, S. O., Roepstorff, P., Protein Sci. 1995, 4, 394-404.
14. Henderson, L. E., Oroszlan, S., Konigsberg, W., Anal. Biochem. 1979, 93, 153-157.
15. Schuhmacher, M., Master Thesis, Universität Konstanz, 1993.
16. rd Conference on Mass Spectrometry and Allied Topics, Atlanta, GA, USA, May 21-26, 1995, p. 618.
17. Hall, S. C., Smith, D. M., Masiarz, F. R., Soo, V. W., Tran, H. M., Epstein, S. C., Burlingame, A. L., Proc. Natl. Acad. Sci. USA 1993, 90, 1927-1931.
18. Le Maire, M., Deschamps, S., Møller, J. V., Le Caer, J. P., Rossier, J., Anal. Biochem. 1993, 214, 50-57.
19. Przybylski, M., Glocker, M. O., Angew. Chem. Int. Ed. 1996, 35, 4001-4021.
20. Chiari, M., Righetti, P. G., Negri, A., Ceciliani, F., Ronchi, S., Electrophoresis 1992, 13, 882-884.
21. Schägger, H., v. Jagow, G., Anal. Biochem. 1987, 166, 368-379.
22. Klarskov, K., Roepstorff, P., Biol. Mass Spectrom. 1992, 22, 433-440.