Determination of protein-bound palmitate turnover rates using a three-compartment model that formally incorporates [3H]palmitate recycling

Biochemistry

Volumn 43, Issue 38, 2004, Pages 12275-12288

  Qanbar, Riad ^a   Bouvier, Michel ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

METABOLISM; PHYSIOLOGY; RECYCLING;

PALMITOYLATION; TRACERS;

PROTEINS;

CELL PROTEIN; PALMITIC ACID;

ARTICLE; BINDING AFFINITY; COMPARTMENT MODEL; DATA ANALYSIS; ISOTOPE LABELING; MOLECULAR DYNAMICS; PALMITOYLATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION; RECYCLING; TURNOVER TIME;

CELL DIVISION; CELL LINE; HUMANS; KINETICS; MODELS, BIOLOGICAL; PALMITATES; PALMITOYL COENZYME A; PROTEIN BINDING; PROTEINS; TIME FACTORS; TRITIUM;

EID: 4644305386     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049176u     Document Type: Article

References (35)

1. McCabe, J. B., and Berthiaume, L. G. (2001) N-terminal protein acylation confers localization to cholesterol, sphingolipid-enriched membranes but not to lipid rafts/caveolae, Mol. Biol. Cell 12, 3601-3617.
2. Yang, S., Zhang, L., and Huang, Y. (2001) Membrane association and conformational change of palmitoylated G(o)alpha, FEBS Lett. 498, 76-81.
3. O'Dowd, B. F., Hnatowich, M., Caron, M. G., Lefkowitz, R. J., and Bouvier, M. (1989) Palmitoylation of the human beta 2-adrenergic receptor. Mutation of Cys341 in the carboxyl tail leads to an uncoupled nonpalmitoylated form of the receptor, J. Biol. Chem. 264, 7564-7569.
4. Hayashi, M. K., and Haga, T. (1997) Palmitoylation of muscarinic acetylcholine receptor m2 subtypes: reduction in their ability to activate G proteins by mutation of a putative palmitoylation site, cysteine 457, in the carboxyl-terminal tail, Arch. Biochem. Biophys. 340, 376-382.
5. Moffett, S., Mouillac, B., Bonin, H., and Bouvier, M. (1993) Altered phosphorylation and desensitization patterns of a human beta 2-adrenergic receptor lacking the palmitoylated Cys341, EMBO J. 12, 349-356.
6. Palmer, T. M., and Stiles, G. L. (2000) Identification of threonine residues controlling the agonist-dependent phosphorylation and desensitization of the rat A(3) adenosine receptor, Mol. Pharmacol. 57, 539-545.
7. Hawtin, S. R., Tobin, A. B., Patel, S., and Wheatley, M. (2001) Palmitoylation of the vasopressin V1a receptor reveals different conformational requirements for signaling, agonist-induced receptor phosphorylation, and sequestration, J. Biol. Chem. 276, 38139-38146.
8. Kraft, K., Olbrich, H., Majoul, I., Mack, M., Proudfoot, A., and Oppermann, M. (2001) Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization, J. Biol. Chem. 276, 34408-34418.
9. Qanbar, R., and Bouvier, M. (2003) Role of palmitoylation/ depalmitoylation reactions in G-protein-coupled receptor function, Pharmacol. Ther. 97, 1-33.
10. James, G., and Olson, E. N. (1989) Identification of a novel fatty acylated protein that partitions between the plasma membrane and cytosol and is deacylated in response to serum and growth factor stimulation, J. Biol. Chem. 264, 20998-21006.
11. Moffett, S., Adam, L., Bonin, H., Loisel, T. P., Bouvier, M., and Mouillac, B. (1996) Palmitoylated cysteine 341 modulates phosphorylation of the beta2-adrenergic receptor by the cAMP-dependent protein kinase, J. Biol. Chem. 271, 21490-21497.
12. Patterson, S. I. (2002) Posttranslational protein S-palmitoylation and the compartmentalization of signaling molecules in neurons, Biol. Res. 35, 139-150.
13. Bijlmakers, M. J., and Marsh, M. (2003) The on-off story of protein palmitoylation, Trends Cell Biol. 13, 32-42.
14. Kennedy, M. E., and Limbird, L. E. (1994) Palmitoylation of the alpha 2A-adrenergic receptor. Analysis of the sequence requirements for and the dynamic properties of alpha 2A-adrenergic receptor palmitoylation, J. Biol. Chem. 269, 31915-31922.
15. Baker, L. P., and Storm, D. R. (1997) Dynamic palmitoylation of neuromodulin (GAP-43) in cultured rat cerebellar neurons and mouse NIE-115 cells, Neurosci. Lett. 234, 156-160.
16. Lane, S. R., and Liu, Y. (1997) Characterization of the palmitoylation domain of SNAP-25, J. Neurochem. 69, 1864-1869.
17. Wolven, A., Okamura, H., Rosenblatt, Y., and Resh, M. D. (1997) Palmitoylation of p59fyn is reversible and sufficient for plasma membrane association, Mol. Biol. Cell 8, 1159-1173.
18. van de Loo, J. W., Teuchert, M., Pauli, I., Plets, E., Van de Yen, W. J., and Creemers, J. W. (2000) Dynamic palmitoylation of lymphoma proprotein convertase prolongs its half-life, but is not essential for trans-Golgi network localization, Biochem. J. 352 (Part 3), 827-833.
19. Mao, S. Y., and Metzger, H. (1997) Characterization of protein-tyrosine phosphatases that dephosphorylate the high affinity IgE receptor, J. Biol. Chem. 272, 14067-14073.
20. Goldstein, B., Faeder, J. R., Hlavacek, W. S., Blinov, M. L., Redondo, A., and Wofsy, C. (2002) Modeling the early signaling events mediated by FcepsilonRI, Mol. Immunol. 38, 1213-1219.
21. Lu, J. Y., and Hofmann, S. L. (1995) Depalmitoylation of CAAX motif proteins. Protein structural determinants of palmitate turnover rate, J. Biol. Chem. 270, 7251 -7256.
22. Baker, T. L., Booden, M. A., and Buss, J. E. (2000) S-Nitrosocysteine increases palmitate turnover on Ha-Ras in NIH 3T3 cells, J. Biol. Chem. 275, 22037-22047.
23. Baker, T. L., Zheng, H., Walker, J., Coloff, J. L., and Buss, J. E. (2003) Distinct rates of palmitate turnover on membrane-bound cellular and oncogenic H-ras, J. Biol. Chem. 278, 19292-19300.
24. Ponimaskin, E. G., Schmidt, M. F., Heine, M., Bickmeyer, U., and Richter, D. W. (2001) 5-Hydroxytryptamine 4(a) receptor expressed in Sf9 cells is palmitoylated in an agonist-dependent manner, Biochem. J. 353, 627-634.
25. Duncan, J. A., and Oilman, A. G. (2002) Characterization of Saccharomyces cerevisiae acyl-protein thioesterase 1, the enzyme responsible for G protein alpha subunit deacylation in vivo, J. Biol. Chem. 277, 31740-31752.
26. Bligh, E. G., and Dyer, W. J. (1959) A rapid method of total lipid extraction and purification, Can. J. Med. Sci. 37, 911-917.
27. Rouser, G., Fkeischer, S., and Yamamoto, A. (1970) Two-dimensional then layer Chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots, Lipids 5, 494-496.
28. Robinson, P. J., Noronha, J., DeGeorge, J. J., Freed, L. M., Nariai, T., and Rapoport, S. I. (1992) A quantitative method for measuring regional in vivo fatty-acid incorporation into and turnover within brain phospholipids: review and critical analysis, Brain Res. Brain Res. Rev. 17, 187-214.
29. Grange, E., Deutsch, J., Smith, Q. R., Chang, M., Rapoport, S. I., and Purdon, A. D. (1995) Specific activity of brain palmitoyl-CoA pool provides rates of incorporation of palmitate in brain phospholipids in awake rats, J. Neurochem. 65, 2290-2298.
30. Sheppard, C. W. (1962) Basic Principles of the Tracer Method: Introduction to Mathematical Tracer Kinetics, Wiley, New York.
31. Gibaldi, M., and Perrier, D. (1982) Pharmacokinetics, 2nd ed., Dekker, New York.
32. Comar, C. L. (1955) Radioisotopes in Biology and Agriculture: Principles and Practice, McGraw-Hill, New York.
33. Tetzloff, S. U., and Bizzozero, O. A. (1998) Palmitoylation of proteolipid protein from rat brain myelin using endogenously generated 18O-fatty acids, J. Biol. Chem. 273, 279-285.
34. Loisel, T. P., Adam, L., Hebert, T. E., and Bouvier, M. (1996) Agonist stimulation increases the turnover rate of beta 2AR-bound palmitate and promotes receptor depalmitoylation, Biochemistry 35, 15923-15932.
35. Mouillac, B., Caron, M., Bonin, H., Dennis, M., and Bouvier, M. (1992) Agonist-modulated palmitoylation of beta 2-adrenergic receptor in Sf9 cells, J. Biol. Chem. 267, 21733-21737.