Extreme scale-down of expanded bed adsorption: Purification of an antibody fragment directly from recombinant E. coli culture

Biotechnology and Bioengineering

Volumn 87, Issue 5, 2004, Pages 641-647

  Willoughby, Nik ^a   Martin, Phillip ^a   Titchener Hooker, Nigel ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Expanded bed adsorption; Process mimic; Scale down

Indexed keywords

ADSORPTION; BIOTECHNOLOGY; CELL CULTURE; DATA ACQUISITION; ESCHERICHIA COLI; FLOWCHARTING; HYDRODYNAMICS; ION EXCHANGE;

BINDING ISOTHERMS; EXPANDED BED ADSORPTION; ION EXCHANGE CHROMATOGRAPHY;

ANTIBODIES;

IMMUNOGLOBULIN F(AB) FRAGMENT;

ARTICLE; BINDING AFFINITY; CHEMICAL REACTION KINETICS; ELUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPANDED BED ADSORPTION; FERMENTATION; HYDRODYNAMICS; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCESS DEVELOPMENT; PURIFICATION; SCALE DOWN;

ADSORPTION; ANTIBODIES; CHROMATOGRAPHY; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EQUIPMENT DESIGN; ESCHERICHIA COLI; FERMENTATION; KINETICS; MOLECULAR WEIGHT; RECOMBINANT PROTEINS;

EID: 4644303364     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20173     Document Type: Article

References (10)

1. Amersham Biosciences. 1998. Expanded bed adsorption-principles and methods. Handbook 18-1124-26 AB, Uppsala, Sweden.
2. Bowering LC, Bracewell DG, Keshavarz-Moore E, Hoare M, Weir ANC. 2002. Comparison of techniques for monitoring antibody fragment production in E. coli fermentation cultures. Biotechnol Prog 18:1431-1438.
3. Boychyn M, Yim SSS, Ayazi Shamlou P, Bulmer M, More J, Hoare M. 2001. Characterization of flow intensity in continuous centrifuges for the development of laboratory mimics. Chem Eng Sci 56:4759-4770.
4. Datar RV, Rosen CG. 1990. Downstream process economics. In: Asenjo JA, editor. Separation processes in biotechnology. New York: Marcel Dekker. p 741-793.
5. Fish NM, Lilly MD. 1984. The interactions between fermentation and protein recovery. Bio/Technol 2:623-627.
6. Ghose S, Chase H. 200Oa. Expanded bed chromatography of proteins in small diameter columns. I. Scale down and validation. Bioseparation 9:21-28.
7. Ghose S, Chase H. 200Ob. Expanded bed chromatography of proteins in small diameter columns. II. Method developments and scale-up. Bioseparation 9:29-36.
8. nd edition. Wiley, New York.
9. Maybury JP, Hoare M, Dunnill P. 1998. The performance of a scaled down industrial stack centrifuge with a reduced feed material requirement. Bioproc Eng 18:191-199.
10. Reynolds T, Boychyn M, Sanderson T, Bulmer M, More J, Hoare M. 2003. Scale-down of continuous filtration for rapid bioprocess design: recovery and dewatering of protein precipitate suspensions. Biotechnol Bioeng 83:454-464.