Multistep sequential mechanism of Escherichia coli helicase PriA protein-ssDNA interactions. Kinetics and energetics of the active ssDNA-searching site of the enzyme

Biochemistry

Volumn 43, Issue 34, 2004, Pages 11002-11016

  Galletto, Roberto ^a   Jezewska, Maria J ^a   Bujalowski, Wlodzimierz ^a,b  

^a UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

^b University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONFORMATIONS; DERIVATIVES; DNA; ENZYME KINETICS; ESCHERICHIA COLI; FLUORESCENCE; GLYCEROL; OLIGOMERS; SALTS;

BINDING SITES; EQUILIBRIUM CONSTANT; INTRAMOLECULAR TRANSITIONS; ION RELEASE;

ENZYMES;

ADENOSINE; ADENYLYLIMIDODIPHOSPHATE; BACTERIAL PROTEIN; ENZYME; HELICASE; OLIGOMER; PROTEIN PRIA; SINGLE STRANDED DNA; UNCLASSIFIED DRUG; ADENOSINE TRIPHOSPHATASE; DIVALENT CATION; ESCHERICHIA COLI PROTEIN; GLYCEROL; MAGNESIUM CHLORIDE; PRIA PROTEIN, E COLI; SODIUM CHLORIDE;

ARTICLE; BINDING SITE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; DNA BINDING; DNA STRUCTURE; ENZYME BINDING; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STABILITY; ESCHERICHIA COLI; FLUORESCENCE; ION TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN DOMAIN; WATER TRANSPORT; CHEMICAL MODEL; CHEMISTRY; ENZYME SPECIFICITY; KINETICS; METABOLISM; METHODOLOGY; PROTEIN BINDING; PROTEIN CONFORMATION; SPECTROFLUOROMETRY; THERMODYNAMICS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ADENOSINE TRIPHOSPHATASES; ADENYLYL IMIDODIPHOSPHATE; BINDING SITES; CATIONS, DIVALENT; DNA HELICASES; DNA, SINGLE-STRANDED; ENZYME STABILITY; ESCHERICHIA COLI PROTEINS; GLYCEROL; KINETICS; MAGNESIUM CHLORIDE; MODELS, CHEMICAL; PROTEIN BINDING; PROTEIN CONFORMATION; SODIUM CHLORIDE; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

EID: 4644283081     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi049378q     Document Type: Article

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