Improved growth in a field trial of transgenic hybrid poplar overexpressing glutamine synthetase

New Phytologist

Volumn 164, Issue 1, 2004, Pages 137-145

  Zhong, Ping Jing ^a   Gallardo, Fernando ^a   Pascual, María Belén ^a   Sampalo, Rafael ^a   Romero, José ^b   De Navarra, Augusto Torres ^b   Cánovas, Francisco M ^a  

^a Campus de Teatinos   (Spain)

^b Edificio Bic Euronova   (Spain)

Author keywords

Field trial; Glutamine biosynthesis; Nitrogen metabolism; Populus; Transgenic trees

Indexed keywords

ENZYME;

POPULUS; POPULUS BEROLINENSIS;

EID: 4644268847     PISSN: 0028646X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-8137.2004.01173.x     Document Type: Article

References (34)

1. Bauer D, Biehler K, Fock H, Carrayol E, Hirel B, Migge A, Becker TW. 1997. A role for cytosolic glutamine synthetase in the remobilization of leaf nitrogen during water stress in tomato. Physiologia Plantarum 99: 241-248.
2. Bhalerao R, Keskitalo J, Sterky F, Erlandsson R, Bjorkbacka H, Birve SJ, Karlsson J, Gardestrom P, Gustafsson P, Lundeberg J, Jansson S. 2003. Gene expression in autumn leaves. Plant Physiology 131: 430-442.
3. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
4. Bradshaw HD, Ceulemans R, Davis J. 2000. Emerging model systems in plant biology: poplar (Populus) as a model forest tree. Journal of Plant Growth Regulation 19: 306-313.
5. Cánovas FM, Dumas-Gaudot E, Recorbet G, Jorrin J, Mock H-P, Rossignol M. 2004. Plant proteome analysis. Proteomics 4: 285-298.
6. Cantón FR, García-Gutiérrez A, Crespillo R, Cánovas FM. 1996. High-level expression of Pinus sylvestris glutamine synthetase in Escherichia coli. Production of polyclonal antibodies against the recombinant protein and expression in pine seedlings. FEBS Letters 393: 205-210.
7. Coleman GD, Chen THH, Ernst SG, Fuchigami. 1991. Photoperiod control of poplar bark storage protein accumulation. Plant Physiology 96: 686-692.
8. Confalonieri M, Balestrazzi A, Bisoffi S, Carbonera D. 2003. In vitro culture and genetic engineering of Populus spp. synergy for forest tree improvement. Plant Cell Tissue Organ and Culture 72: 109-138.
9. Cren M, Hirel B. 1999. Glutamine synthetase in higher plants. Regulation of gene and protein expression from the organ to the cell. Plant Cell Physiology 40: 1187-1193.
10. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28: 350-356.
11. Fu J, Sampalo R, Gallardo F, Cánovas FM, Kirby E. 2003. Assembly of cytosolic pine glutamine synthetase holoenzyme in leaves of transgenic poplar leads to enhanced vegetative growth. Plant, Cell & Environment 26: 411-418.
12. Fuentes SI, Allen DJ, Ortiz-López A, Hernández G. 2001. Over-expression of cytosolic glutamine synthetase increases photosynthesis and growth at low nitrogen concentrations. Journal of Experimental Botany 52: 1071-1081.
13. Gallardo F, Fu J, Cantón FR, García-Gutiérrez A, Cánovas FM, Kirby EG. 1999. Expression of a conifer glutamine synthetase gene in transgenic poplar. Planta 210: 19-26.
14. Gallardo F, Fu J, Jing ZP, Kirby EG, Canovas FM. 2003. Genetic modification of amino acid metabolism in woody plants. Plant Physiology and Biochemistry 41: 587-594.
15. Gallardo F, Gálvez S, Quesada MA, Cánovas FM, Núñez de Castro I. 1988. Glutamine synthetase activity during the ripening of tomato fruit. Plant Physiology and Biochemistry 26: 747-752.
16. Gálvez S, Gallardo F, Cánovas FM. 1990. The occurrence of glutamine synthetase isoenzymes in tomato plants is correlated to plastid differentiation. Journal of Plant Physiology 137: 1-4.
17. García-Gutiérrez A, Cantón FR, Gallardo F, Sánchez-Jiménez F, Cánovas FM. 1995. Expression of ferredoxin-dependent glutamate synthase in dark-grown pine seedlings. Plant Molecular Biology 27: 115-128.
18. García-Gutiérrez A, Dubois F, Cantón FR, Gallardo F, Sangwan RS, Cánovas FM. 1998. Two different modes of early development and nitrogen assimilation in gymnosperm seedlings. Plant Journal. 13: 187-199.
19. Hirel B, Bertin P, Quilleré I, Bourdoncle W, Attagnant C, Dellay C, Gouy A, Cadiou S, Retailliau C, Falque M, Gallais A. 2001. Towards a better understanding of the genetic and physiological basis for nitrogen use efficiency in maize. Plant Physiology 125: 1258-1270.
20. Kawakami N, Watanabe A. 1988. Senescence-specific increase in cytosolic glutamine synthetase and its mRNA in radish cotyledons. Plant Physiology 88: 1430-1434.
21. Kirk K, Obst JR. 1988. Lignin determination. Methods in Enzymology 161: 87-101.
22. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
23. Langheinrich U, Tischner R. 1991. Vegetative storage proteins in poplar. Induction and characterization of a 32- and a 36-kilodalton polypeptide. Plant Physiology 97: 1017-1025.
24. Lea PJ. 1997. Primary nitrogen metabolism. In: Dey PM, Harborne JB, eds. Plant biochemistry. San Diego, CA, USA: Academic Press, 273-313.
25. Miflin B, Habash DZ. 2002. The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops. Journal of Experimental Botany 53: 979-987.
26. Miflin BJ, Lea PJ. 1980. Ammonia assimilation. In: Miflin BJ, ed. The biochemistry of plants, vol 5. San Diego, CA, USA: Academic Press, 169-202.
27. Obara M, Sato T, Yamaya T. 2001. Mapping of QTLs associated with cytosolic glutamine synthetase and NADH-glutamate synthase in rice (Oryza sativa L.). Journal of Experimental Botany 52: 1209-1217.
28. Pérez-García A, Cánovas FM, Gallardo F, Hirel B, de Vicente A. 1995. Differential expression of glutamine synthetase isoforms in tomato detached leaflets infected with Pseudomonas syringae pv. tomato. Molecular Plant-Microbe Interactions 8: 96-103.
29. Pilate G, Guiney E, Holt K, Petit-Conil M, Lapierre C, Leple JC, Pollet B, Mila I, Webster EA, Mastorp HG, Hopkins DW, Jouanin L, Boerjan W, Schuch W, Cornu D, Halpin C. 2002. Field and pulping performances of transgenic trees with altered lignification. Nature Biotechnology 20: 607-612.
30. Sauter JJ, van Cleve B. 1992. Seasonal variation of amino acids in the xylem sap of Populus x canadensis and its relation to protein body mobilization. Trees 7: 26-32.
31. Suárez MF, Avila C, Gallardo F, Cantón FR, García-Gutiérrez A, Claros MG, Cánovas FM. 2002. Molecular and enzymatic analysis of ammonium assimilation in woody plants. Journal of Experimental Botany 53: 891-904.
32. Taylor G. 2002. Populus: Arabidopsis for forestry. Do we need a model tree? Annals of Botany 90: 687-766.
33. Wetzel S, Demmers C, Greenwood JS. 1989. Seasonally fluctuating bark proteins are a potential form of nitrogen storage in three temperate hardwoods. Planta 178: 275-281.
34. Zhu B, Coleman GD. 2001. Phytochrome-mediated photoperiod perception, shoot growth, glutamine, calcium and protein phosphorylation influence the activity of the poplar bark storage protein gene promoter (bspA). Plant Physiology 126: 342-251.