Detection and identification of Actinobacillus pleuropneumoniae serotypes 1, 2, and 8 by multiplex PCR

Journal of Clinical Microbiology

Volumn 42, Issue 9, 2004, Pages 4344-4348

  Schuchert, Jennifer A ^a   Inzana, T J ^a   Angen, O ^b   Jessing, S ^b  

^a CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; CAPSULAR POLYSACCHARIDE; DNA FRAGMENT; POLYSACCHARIDE; UNCLASSIFIED DRUG;

ACTINOBACILLUS PLEUROPNEUMONIAE; ARTICLE; BACTERIAL MEMBRANE; BACTERIOLOGY; BIOSYNTHESIS; DNA DETERMINATION; GENE AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEROTYPE;

ACTINOBACILLUS PLEUROPNEUMONIAE; BASE SEQUENCE; DNA PRIMERS; DNA, BACTERIAL; ELECTROPHORESIS, AGAR GEL; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLASMIDS; POLYMERASE CHAIN REACTION; SEROTYPING;

ACTINOBACILLUS; ACTINOBACILLUS PLEUROPNEUMONIAE; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 4644241497     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.9.4344-4348.2004     Document Type: Article

References (24)

1. Blackall, P. J., H. B. L. M. Klaasen, H. van den Bosch, P. Kuhnert, and J. Frey. 2002. Proposal of a new serovar of Actinobacillus pleuropneumoniae: serovar 15. Vet. Microbiol. 84:47-52.
2. Clarke, B. R., R. Pearce, and L. S. Roberts. 1999. Genetic organization of the Escherichia coli K10 capsule gene cluster: identification and characterization of two conserved regions in group III capsule gene clusters encoding polysaccharide transport functions. J. Bacteriol. 181:2279-2285.
3. Fath, M. J., and R. Kolter. 1993. ABC transporters: bacterial exporters. Microbiol. Rev. 57:995-1017.
4. Frosch, M., U. Edwards, K. Bousset, B. Krausse, and C. Weisgerber. 1991. Evidence for a common molecular origin of the capsule gene loci in gram-negative bacteria expressing group II capsular polysaccharides. Mol. Microbiol. 5:1251-1263.
5. Gram, T., P. Ahrens, and J. P. Nielsen. 1996. Evaluation of a PCR for detection of Actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. Vet. Microbiol. 51:95-104.
6. Gram, T., and P. Ahrens. 1998. Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein. J. Clin. Microbiol. 36:443-448.
7. Gram, T., P. Ahrens, M. Andreasen, and J. P. Nielsen. 2000. An Actinobacillus pleuropneumoniae PCR typing system based on the apx and omlA genes-evaluation of isolates from lungs and tonsils of pigs. Vet. Microbiol. 75:43-57.
8. Hennessy, K. J., J. J. Iandolo, and B. W. Fenwick. 1993. Serotype identification of Actinobacillus pleuropneumoniae by arbitrarily primed polymerase chain reaction. J. Clin. Microbiol. 31:1155-1159.
9. Innis, M. A., D. H. Gelfand, and J. J. Sninsky. 1995. PCR strategies. Academic Press, Inc., San Diego, Calif.
10. Inzana, T. J., and B. Mathison. 1987. Serotype specificity and immunogenicity of the capsular polymer of Haemophilus pleuropneumoniae. Infect. Immun. 55:1580-1587.
11. Inzana, T. J. 1995. Simplified procedure for preparation of sensitized latex particles to detect capsular polysaccharides: application to typing and diagnosis of Actinobacillus pleuropneumoniae. J. Clin. Microbiol. 33:2297-2303.
12. Jessing, S. G., Ø. Angen, and T. J. Inzana. 2003. Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6. J. Clin. Microbiol. 41:4095-4100.
13. Lo, R. Y. C., L. J. McKerral, T. L. Hills, and M. Kostrzynska. 2001. Analysis of the capsule biosynthesis locus of Mannheimia (Pasteurella) haemolytica A1 and proposal of a nomenclature system. Infect. Immun. 69:4458-4464.
14. Lo, T. M., C. K. Ward, and T. J. Inzana. 1998. Detection and identification of Actinobacillus pleuropneumoniae serotype 5 by multiplex PCR. J. Clin. Microbiol. 36:1704-1710.
15. Nicolet, J. 1988. Taxonomy and serological identification of Actinobacillus pleuropneumoniae. Can. Vet. J. 29:578-579.
16. Perry, M. B., E Altman, J. R. Brisson, L. M. Beynon, and J. C. Richards. 1990. Structural characteristics of the antigenic capsular polysaccharide and lipopolysaccharide involved in serological classification of Actinobacillus pleuropneumoniae strains. Serodiagn. Immunother. Infect. Dis. 4:299-308.
17. Roberts, I. S., R. Mountford, N. High, D. Bitter-Suerman, K. Jann, K. N. Timmis, and G. J. Boulnois. 1986. Molecular cloning and analysis of the genes for production of the K5, K7, K12, and K92 capsular polysaccharides of Escherichia coli. J. Bacteriol. 168:1228-1233.
18. Russo, T. A., S. Wenderoth, U. B. Carlino, J. M. Merrick, and A. J. Lesse. 1998. Identification, genomic organization, and analysis of the group III capsular polysaccharide genes kpsD, kpsM, kpsT, and kpsE from an extraintestinal isolate of Escherichia coli (CP9, O/4/K54/H5). J. Bacteriol. 180:338-349.
19. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed., vol. 1-3. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20. Savoye, C., J. L. Jobert, F. Berthelot-Herault, A. M. Keribin, R. Cariolet, H. Morvan, F. Madec, and M. Kobish. 2000. A PCR assay used to study aerosol transmission of Actinobacillus pleuropneumoniae from samples of live pigs under experimental conditions. Vet. Microbiol. 73:337-347.
21. Schaller, A., S. P. Djordjevic, G. J. Eamens, W. A. Forbes, R. Kuhn, P. Kuhnert, M. Gottschalk, J. Nicolet, and J. Frey. 2001. Identification and detection of Actinobacillus pleuropneumoniae by PCR based on the gene apxIVA. Vet. Microbiol. 79:47-62.
22. Taylor, D. J. 1999. Actinobacillus pleuropneumoniae, p. 343-354. In B. E. Straw, S. D'Allaire, W. L. Mengeling, and D. J. Taylor (ed.), Diseases of swine. Iowa State University Press, Ames, Iowa.
23. Ward, C. K., and T. J. Inzana. 1997. Identification and characterization of a DNA region involved in the export of capsular polysaccharide by Actinobacillus pleuropneumoniae serotype 5a. Infect. Immun. 65:2491-2496.
24. Ward, C. K., M. L. Lawerence, H. P. Veit, and T. J. Inzana. 1998. Cloning and mutagenesis of a serotype-specific DNA region involved in encapsulation and virulence of Actinobacillus pleuropneumoniae serotype 5a: concomitant expression of serotype 5a and 1 capsular polysaccharides in recombinant A. pleuropneumoniae serotype 1. Infect. Immun. 66:3326-3336.