Identification and characterization of a DNA region involved in the export of capsular polysaccharide by Actinobacillus pleuropneumoniae serotype 5a

Infection and Immunity

Volumn 65, Issue 6, 1997, Pages 2491-2496

  Ward, Christine K ^a,c   Inzana, Thomas J ^a,b  

^a CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

^b VIRGINIA MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE   (United States)

^c UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIUM LIPOPOLYSACCHARIDE;

ACTINOBACILLUS PLEUROPNEUMONIAE; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL MEMBRANE; BACTERIAL VIRULENCE; BACTERIUM IDENTIFICATION; HAEMOPHILUS INFLUENZAE TYPE B; MEMBRANE STRUCTURE; MOLECULAR CLONING; NEISSERIA MENINGITIDIS; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEROTYPE;

EID: 0030981155     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.65.6.2491-2496.1997     Document Type: Article

References (48)

1. Altschul, S. F., W. Glsh, W. Miller, E. W. Myers, and D. L. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
2. Aydintug, M. K., T. J. Inzana, T. Letonja, W. C. Da vis, and L. B. Cornell. 1989. Cross-reactivity of monoclonal antibodies to Escherichia coli J5 with heterologous gram-negative bacteria and extracted lipopolysaccharides. J. Infect. Dis. 160: 846-857.
3. Boulnois, G. J., and I. S. Roberts. 1990. Genetics of capsular polysaccharide production in bacteria. Curr. Top. Microhiol. Immunol. 150: 1-18.
4. Boulnois, G. J., I. S. Roberts, R. Hodge, K. R. Hardy, K. B. Jann, and K. N. Timmis. 1987. Analysis of the K1 capsule biosynthesis genes of Eschcrichia coli: definition of three functional regions for capsule production. Mol. Gen. Genet. 208: 242-246.
5. Bronner, D., B. R. Clarke, and C. Whitfleld. 1994. Identification of an ATP-binding cassette transport system required for the translocation of lipopolysaccharide O-antigen side-chains across the cytoplasmic membrane of Klebsiella pneunmoniae serotype O1. Mol. Microbiol. 14: 505-519.
6. Cleslcwicz, M. J., S. M. Steenbergen, and E. R. Vimr. 1993. Cloning, sequencing, expression, and complementation analysis of the Excherichia coli K1 kps region 1 gene, kpsE, and identification of an upstream open reading frame encoding a protein with homology to GutQ. J. Bacteriol. 175: 8018-8023.
7. Dower, W. J., J. F. Miller, and C. W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporaiion. Nucleic Acids Res. 16: 6127-6145.
8. Path, M. J., and R. Kolter. 1993. ABC transporters: bacterial exporters. Microbiol. Rev. 57: 995-1017.
9. Frosch, M., U. Edwards, K. Boussel, B. Krausse, and C. Weisgerber. 1991. Evidence for a common molecular origin of the capsule gene loci in gramnegative bacteria expressing group II capsular polysaccharides. Mol. Microbiol. 5: 1251-1263.
10. Frosch, M., C. Weisgerber, and T. F. Meyer. 1989. Molecular characterization and expression in Escherichia coli of the gene complex encoding the polysaccharide capsule of Neisseria meningitidis group B. Proc. Natl. Acad. Sci. USA 86: 1669-1673.
11. Huwley, D. K., and W. R. McClure. 1983. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 11: 2237-2255.
12. Higgins, C. F. 1992. ABC transporters: from microorganisms to man, Annu. Rev. Cell Biol. 8: 67-113.
13. Hoiseth, S. K., C. J. Connelly, and E. R. Moxon. 1985, Genetics of spontaneous, high-frequency loss of b capsule expression in Haemophilus influenzae. Infect. Immun. 49: 389-395.
14. Inzana, T. J., J. Ma, T. Workman, R. P. Gogolewski, and P. Anderson. 1988. Virulence properties and protective efficacy of the capsular polymer of Hae- mophilus (Actinobacillus) pleuropneumoniae serotype 5. Infect. Immun. 56: 1880-1884.
15. Inzana, T. J., and B. Mathison. 1987. Serotvpe specificity and immunogenicity of the capsular polymer of Haemophilus pleuropneumoniae serotype 5. Infect. Immun. 55: 1580-1587.
16. Inzana, T. J., J. Todd, and H. P. Veit. 1993. Safety, stability, and efficacy of noncapsulated mutants of Actinobacillus pleuropneumoniae for use in live vaccines. Infect. Immun. 61: 1682-1686.
17. Ish-Horowicz, D., and J. F. Burke. 1981. Rapid and efficient cosmid cloning. Nucleic Acids Res. 9: 2989-2998.
18. Jann, B., and K. Jann. 1990. Structure and biosynthesis of the capsular antigens of Escherchia coli. Curr. Top. Microbiol. Immunol. 150: 19-42.
19. Jann, K., and B. Jann. 1992. Capsules of Eschttrichia coli, expression and biological significance. Can. J. Microbiol. 38: 705-710.
20. Kroll, J. S, 1992. The genetics of encapsulation in Haemophilus influenzae. J. Infect. Dis. 165(Suppl. 1):S93-S96.
21. Kroll, J. S., B. Loynds, L. N. Bruphy, and E. R. Moxon. 1990. The bex locus in encapsulated Haemophilus influenzae: a chromosomal region involved in capsule polysaccharide export. Mol. Microbiol. 4: 1853-1862.
22. Kroll, J. S., S. Zamze, B. Loynds, and E. R. Moxon. 1989. Common organization of chromosomal loci for production of different capsular polysaccharides in Haemophilus influenzae. J. Bacteriol. 171: 3343-3347.
23. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157: 1005-1032.
24. Lucas, A. H. 1988. Expression of crossreactive idiotypes by human antibodies specific for the capsular polysaccharide of Haemophilus influenzae type b. J. Clin. Invest. 81: 480-486.
25. Nielsen, R. 1986. Serological characterization of Actinobacillus pleumpneumoniae strains and proposal of a new serotype: serotype 12. Acta Vet. Scand. 27: 453-455.
26. Pavelka, M. S., L. F. Wright, and R. P. Silver. 1991. Identification of two genes, kpsM and kpsT. in region 3 of the polysialic acid gene cluster of Escherichia coli K1. J. Bacteriol. 173: 4603-4610.
27. Pazzani, C., C. Rosenow, G. J. Boulnois, D. Bronner, K. Jann, and I. S. Roberts. 1993. Molecular analysis of region 1 of the Escherichia coli K5 antigen gene cluster: a region encoding proteins involved in cell surface expression of capsular polysaccharide. J. Bacteriol. 175: 5978-5983.
28. Pearce, R., and I. S. Roberts. 1995. Cloning and analysis of gene clusters for production of the Escherichia coli K10 and K54 antigens: identification of a new group of serA-linked capsule gene clusters. J. Bacteriol. 177: 3992-3997.
29. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57: 50-108.
30. Reizer, J., A. Reizer, and M. H. Saier, Jr. 1992. A new subfamily of bacterial ABC-type transport systems catalyzing export of drugs and carbohydrates. Protein Sci. 1: 1326-1332.
31. Ried, J. L., and A. Collmer. 1987. An nptl-sac'R cartridge for constructing directed, unmarked mutations in gram-negative bacteria by marker exchange-eviction mutagenesis, Gene 57:239-246.
32. Roberts, I., R. Mountford, N. High, D. Bitter-Suermann, K. Jann, K. Timmis, and G. Boulnois. 1986. Molecular cloning and analysis of genes for production of K5, K7, K12. and K92 capsular polysaccharides in Escherichia coll. J. Bacteriol. 168: 1228-1233.
33. Roberts, I. S., R. Mountford, R. Hudge, K. Jann. and G. J. Boulnois. 1988. Common organization of gone clusters for production of different capsular polysaccharides (K antigens) in Eschericha coli. J. Bacteriol, 170: 1305-1310.
34. Roof, M. B., R. DenHoed, D. Cleveringa, J. T. Hoick, and T. J. Inzana. 1996. Safety and efficacy of an avirulent live Actinohadlhts plcuropncumonim.1 isolate in pigs, abstr. 195. In 77th Conference of Research Workers in Animal Diseases. Chicago. Ill.
35. Rosenberg, M., and D. Court. 1979. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu. Rev. Genet. 13: 319-353.
36. Rosendal, S. D. A. Boyd, and K. A. Gilbride. 1985. Comparative virulence of porcine Haemophilus bacteria. Can. J. Comp. Med. 49: 68-74.
37. Rosenow, C., F. Esumah, I. S. Roberts, and K. Jann. 1995. Characterization and localization of the KpsE protein of Escherichia coli K5, which is involved in polysaccharide export. J. Bacieriol. 177: 1137-1143.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1489, Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, N.Y.
39. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467.
40. Shine, J., and L. Dalgarno. 1974. The 3′ terminal sequence of Eschcrichia coli 16S ribosomal RNA: complementarity to nonsense triplets and the ribosome binding sites. Proc. Natl. Acad. Sci. USA 71: 1342-1346.
41. Smith, A. N., G. J. Boulnois, and I. S. Roberts. 1990. Molecular analysis of the Escherfchia coli K5 kps locus: identification and characterization of an inner-membrane capsular polysaccharide transport system. Mol. Microbiol. 4: 1863-1869.
42. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 503-517.
43. Stevens, M. P., P. Hänfling, B. Jann, K. Jann, and I. S. Roberts. 1994. Regulation of Escherichia coli K5 capsular polysaccharide expression: evidence for involvement of RfaH in the expression of group II capsules. FEMS Microbiol. Lett. 124: 93-98.
44. Tartof, K. D., and C. A. Hobbes. 1987. Improved media for growing plasmid and cosmid clones. Bethesda Res. Lab. Focus 9(2) : 12.
45. Walker, J. E., M. Sarste, M. J. Runswlck, and N. J. Gay. 1982. Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes, and a common nucleotide binding fold. EMBO J. 1: 945-951.
46. Ward, C. K., and T. J. Inzana. 1994. Resistance of Actinobacillus pleuropneumonia? to bactericidal antibody and complement is mediated by capsular polysaccharide and blocking antibody specific for lipopolysaccharide. J. Immunol. 153: 2110-2121.
47. Ward, C. K., and T. J. Inzana. 1996. Mutations in capsular polysaccharide biosynthesis render Actinobacillus pleuropneumoniae avirulent, abstr. 25. In Proceedings of the Haemophilus, Actinobacillust Pastvurella 96 International Conference. Acapulco. Mexico.
48. Zhen, L., and R. T. Swank. 1993. A simple and high yield method for recovering DNA from agarose gels. BioTechniqucs 14: 894-898.