Kinetic mechanism of histidine-tagged homocitrate synthase from Saccharomyces cerevisiae

Biochemistry

Volumn 43, Issue 37, 2004, Pages 11790-11795

  Andi, Babak ^a   West, Ann H ^a   Cook, Paul F ^a  

^a UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE; HYDROLYSIS; ORGANIC COMPOUNDS; REACTION KINETICS; TITRATION;

HISTIDINE-TAGGED HOMOCITRATE SYNTHASE (HCS); SYNERGISM;

ENZYMES;

2 OXOGLUTARIC ACID; ACETYL COENZYME A; CITRIC ACID; COENZYME A; FUNGAL ENZYME; HISTIDINE; HOMOCITRATE SYNTHASE; OXALOACETIC ACID; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; DISSOCIATION CONSTANT; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; YEAST;

ACETYL COENZYME A; ALPHA-CYCLODEXTRINS; CYCLODEXTRINS; LIGANDS; MATHEMATICS; OXALOACETIC ACIDS; OXO-ACID-LYASES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 4644223096     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi048766p     Document Type: Article

References (14)

1. Johansson, E., Steffens, J. J., Lindqvist, Y., and Schneider, G. (2000) Crystal structure of Saccharopine reductase from Magnaporthe grisea, an enzyme of the α-aminoadipate pathway of lysine biosynthesis, Structure 8, 1037-1047.
2. Bhattacharjee, J. K. (1992) Evolution of α-aminoadipate pathway for the synthesis of lysine in fungi, in Evolution of Metabolic Function (Mortlock, R. P., Ed.) pp 47-80, CRC Press, Boca Raton.
3. Bhattacharjee, J. K. (1985) α-Aminoadipate pathway for the biosynthesis of lysine in lower eukaryotes, Crit. Rev. Microbiol. 12, 131-151.
4. Umbarger, H. E. (1987) Amino acid biosynthesis and its regulation, Annu. Rev. Biochem. 47, 532-606.
5. Vogel, H. J. (1960) Two modes of lysine synthesis among lower fungi: evolutionary significance, Biochim. Biophys. Acta 41, 172-173.
6. Ramos, F., Verhasselt, P., Feller, A., Peeters, P., Wach, A., Dubois, E., and Volckaert, G. (1996) Identification of a gene encoding a homocitrate synthase isoenzyme of Saccharomyces cerevisiae, Yeast 12, 1315-1320.
7. Tracy, J., and Kohlhaw, G. (1975) Reversible, coenzyme-A-mediated inactivation of biosynthesis condensing enzymes in yeast: A possible regulatory mechanism, Proc. Natl. Acad. Sci. U.S.A. 72, 1802-1805.
8. Gaillardin, G. M., Poirier, L. and Heslot, H. (1976) A kinetic study of homocitrate synthase activity in the yeast Saccharomycopsis lipolytica, Biochim. Biophys. Acta 422, 390-406.
9. Jaklitsch, W. M., and Kubicek, C. P. (1990) Homocitrate synthase from Penicillium chrysogenum, localization, purification of the cytosolic isoenzyme, and sensitivity to lysine, Biochem. J. 269, 247-253.
10. Wulandari, A. P., Miyazaki, J., Kobashi, N., Nishiyama, M., Hoshino T., and Yamane, H. (2002) Characterization of bacterial homocitrate synthase involved in lysine biosynthesis, FEBS Lett. 522, 35-40.
11. Andi, B., West, A. H., and Cook, P. F. (2004) Stabilization and characterization of histidine-tagged homocitrate synthase from Saccharomyces cerevisiae, Arch. Biochem. Biophys. 421, 243-254.
12. Cleland, W. W. (1963) The kinetics of enzyme-catalyzed reactions with two or more substrates or products I. Nomenclature and rate equations, Biochim. Biophys. Acta 67, 104-137.
13. Cleland, W. W. (1977) Determining the chemical mechanisms of enzyme-catalyzed reactions by kinetic studies, Adv. Enzymol. 45, 273-387.
14. Berman, H. M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T. N., Weissig, H., Shindyalov, I. N., and Bourne, P. E. (2000) The protein data bank, Nucleic Acids Res. 28, 235-242.