Direct measure of fluorescence intensity for efficient receptor-binding assay: Conjugates of ethinylcarboxyestradiol and 5(and 6)-carboxyfluorescein via α,ω-diaminoalkanes as a tracer for estrogen receptor

Journal of Biochemistry

Volumn 143, Issue 6, 2008, Pages 781-792

  Asai, Daisuke ^a,c   Tokunaga, Takatoshi ^a   Kondo, Kaoru ^b   Kawaguchi, Tsutomu ^a   Takayanagi, Sayaka ^a   Shinmyozu, Teruo ^a   Nakai, Makoto ^b   Yakabe, Yoshikuni ^b   Shimohigashi, Yasuyuki ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b CHEMICALS EVALUATION AND RESEARCH INSTITUTE   (Japan)

^c ST MARIANNA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Endocrine disruptors; Estrogen receptor; Fluorescence intensity; Fluorescent tracer; Receptor binding assay

Indexed keywords

17 ALPHA ETHINYLCARBOXYESTRADIOL; 6 CARBOXYFLUORESCEIN; ALKANE DERIVATIVE; ENDOCRINE DISRUPTOR; ESTRADIOL DERIVATIVE; ESTROGEN; ESTROGEN RECEPTOR; HYDROGEN;

ARTICLE; BINDING AFFINITY; BINDING ASSAY; CONTROLLED STUDY; CROSS LINKING; FLUORESCENCE; FLUORESCENCE POLARIZATION; HUMAN; HUMAN CELL; ISOTOPE LABELING; NONHUMAN; RECEPTOR BINDING; SYNTHESIS;

ALKANES; BINDING, COMPETITIVE; BIOLOGICAL ASSAY; ESTRADIOL; ESTROGEN RECEPTOR ALPHA; FLUORESCEINS; FLUORESCENCE; HUMANS; RADIOLIGAND ASSAY;

EID: 46349108126     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvn031     Document Type: Article

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