Chitin binding by Thermobifida fusca cellulase catalytic domains

Biotechnology and Bioengineering

Volumn 100, Issue 4, 2008, Pages 644-652

  Li, Yongchao ^a   Wilson, David B ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

chitin; Bacterial cellulose (BC); Catalytic domain (CD); Cellulase; Chitinase; Thermobifida fusca

Indexed keywords

CATALYTIC CENTERS; CATALYTIC DOMAINS; CELLO-OLIGOSACCHARIDES; CHITINASE; GENERAL (CO); GLUCOSE RESIDUES; HOMO POLYMERS; HYDROXYL GROUPS; MICRO-FIBRILS; MUTANT ENZYMES; SUGAR RESIDUES; WILD-TYPE (WT); X RAY STRUCTURES;

CADMIUM COMPOUNDS; CELLULOSE; CRACK DETECTION; DATA STORAGE EQUIPMENT; ENZYME ACTIVITY; FOOD ADDITIVES; GLUCOSE; HYDROGEN; MODEL STRUCTURES; POLYSACCHARIDES; SUGAR (SUCROSE); SUGARS;

CHITIN;

ALANINE; ALPHA CHITIN; ARGININE; ASPARTIC ACID; CARBON; CELLULASE; CELLULASE 48A; CELLULASE 5A; CELLULASE 6A; CELLULASE 6B; CELLULASE 9A; CHITIN; CHITINASE; CHITOTETRAOSE; CHITOTRIOSE; CYSTEINE; GLYCINE; HYDROXYL GROUP; LYSINE; OLIGOSACCHARIDE; PHENYLALANINE; SERINE; TRYPTOPHAN; TYROSINE;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HYDROGEN BOND; NONHUMAN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; REACTION ANALYSIS; SOIL MICROFLORA; STRUCTURE ANALYSIS; THERMOBIFIDA FUSCA;

ACTINOMYCETALES; AMINO ACID SUBSTITUTION; BINDING SITES; CATALYTIC DOMAIN; CELLULASE; CELLULOSE; CHITIN; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; OLIGOSACCHARIDES; PROTEIN BINDING; SUBSTRATE SPECIFICITY; TRISACCHARIDES;

CARBON; CATALYSTS; CELLULASE; CELLULOSE; CHITIN; ENZYMES; GLUCOSE; HYDROGEN; HYDROXYL GROUPS; OLIGOSACCHARIDES; POLYMERS; SUCROSE; SUGAR; X RAYS;

BACTERIA (MICROORGANISMS); THERMOBIFIDA FUSCA;

EID: 46249130561     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21808     Document Type: Article

References (29)

1. Barr BK, Wolfgang DE, Piens K, Claeyssens M, Wilson DB. 1998. Active-site binding of glycosides by Thermomonospora fusca endocellulase E2. Biochemistry 37(26):9220-9229.
2. Boraston AB, Bolam DN, Gilbert HJ, Davies GJ. 2004. Carbohydrate-binding modules: Fine-tuning polysaccharide recognition. Biochem J 382(Pt 3):769-781.
3. Davies GJ, Wilson KS, Henrissat B. 1997. Nomenclature for sugar-binding subsites in glycosyl hydrolases. Biochem J 321:557-559.
4. Fukamizo T. 2000. Chitinolytic enzymes: Catalysis, substrate binding, and their application. Curr Protein Pept Sci 1(1): 105-124.
5. Ghose TK. 1987. Measurement of cellulase activities. Pure Appl Chem 59(2):257-268.
6. Horn SJ, Sorbotten A, Synstad B, Sikorski P, Sorlie M, Varum KM, Eijsink VG. 2006. Endo/exo mechanism and processivity of family 18 chit-inases produced by Serratia marcescens. FEBS J 273(3):491-503.
7. Irwin D, Walker L, Spezio M, Wilson D. 1993. Activity studies of eight purified cellulases: Specificity, synergism, and binding domain effects. Biotech Bioeng 42:1002-1013.
8. Irwin D, Shin D, Zhang S, Barr BK, Sakon J, Karplus PA, Wilson DB. 1998. Roles of the catalytic domain and two cellulose binding domains of Thermomonospora fusca E4 in cellulose hydrolysis. J Bacteriol 180(7): 1709-1714.
9. Irwin DC, Zhang S, Wilson DB. 2000. Cloning, expression and characterization of a Family 48 exocellulase, Cel48A, from Thermobifida fusca. Eur J Biochem 267(16):4988-4997.
10. Jung ED, Lao G, Irwin D, Barr BK, Benjamin A, Wilson DB. 1993. DNA sequences and expression in Streptomyces lividans of an exoglucanase gene and an endoglucanase gene from Thermomonospora fusca. Appl Environ Microbiol 59(9):3032-3043.
11. Jung H, Wilson DB, Walker LP. 2002. Binding mechanisms for Thermobifida fusca Cel5A, Cel6B, and Cel48A cellulose binding modules on bacterial microcrystalline cellulose. Biotechnol Bioeng 80(4):380-392.
12. Jung H, Wilson DB, Walker LP. 2003. Binding and reversibility of Thermobifida fusca Cel5A, Cel6B, and Cel48A and their respective catalytic domains to bacterial microcrystalline cellulose. Biotechnol Bioeng 84(2):151-159.
13. Kang HJ, Uegaki K, Fukada H, Ishikawa K. 2007. Improvement of the enzymatic activity of the hyperthermophilic cellulase from Pyrococcus horikoshii. Extremophiles 11(2):251-256.
14. Kumar J, Ethayathulla AS, Srivastava DB, Singh N, Sharma S, Kaur P, Srinivasan A, Singh TP. 2007. Carbohydrate-binding properties of goat secretory glycoprotein (SPG-40) and its functional implications: Structures of the native glycoprotein and its four complexes with chitin-like oligosaccharides. Acta Crystallogr D Biol Crystallogr 63(Pt 4):437-446.
15. Lao G, Ghangas GS, Jung ED, Wilson DB. 1991. DNA sequences of three beta-1, 4-endoglucanase genes from Thermomonospora fusca. J Bacteriol 173(11):3397-3407.
16. Lever M. 1972. A new reaction for colorimetric determination of carbohydrates. Anal Biochem 47:273-279.
17. Li Y, Irwin DC, Wilson DB. 2007. Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Appl Environ Microbiol 73(10):3165-3172.
18. McGinnis K, Wilson DB. 1993. Disulfide arrangement and functional domains of ß-1, 4-endoglucanase E5 from Thermomonospora fusca. Biochemistry 32(32):8157-8161.
19. Morris G, Goodsell D, Halliday R, Huey R, Hart W, Belew R, Olson A. 1998. Automated docking using a Lamarckian genetic algorithm and an empirical binding free energy function. J Comp Chem 19(14): 1639-1662.
20. Noishiki Y, Takami H, Nishiyama Y, Wada M, Okada S, Kuga S. 2003. Alkali-induced conversion of beta-chitin to alpha-chitin. Biomacro-molecules 4(4):896-899.
21. Sakon J, Irwin D, Wilson DB, Karplus PA. 1997. Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca. Nat Struct Biol 4(10):810-817.
22. Shoseyov O, Shani Z, Levy I. 2006. Carbohydrate binding modules: Biochemical properties and novel applications. Microbiol Mol Biol Rev 70(2):283-295.
23. Spezio M, Wilson DB, Karplus PA. 1993. Crystal structure of the catalytic domain of a thermophilic endocellulase. Biochemistry 32:9906-9916.
24. Tharanathan RN, Kittur FS. 2003. Chitin - The undisputed biomolecule of great potential. Crit Rev Food Sci Nutr 43(1):61-87.
25. Vaaje-Kolstad G, Houston DR, Riemen AH, Eijsink VG, Van Aalten DM. 2005. Crystal structure and binding properties of the Serratia marcescens chitin-binding protein C BP21. J Biol Chem 280(12): 11313-11319.
26. Zhang S, Lao G, Wilson DB. 1995. Characterization of a Thermomonospora fusca exocellulase. Biochemistry 34(10):3386-3395.
27. Zhang S, Barr BK, Wilson DB. 2000a. Effects of non-catalytic residue mutations on substrate specificity and ligand binding of Thermobifida fusca endocellulase Cel6A. Eur J Biochem 267:244-252.
28. Zhang S, Irwin DC, Wilson DB. 2000b. Site-directed mutation of non-catalytic residues of Thermobifida fusca exocellulase Cel6B. Eur J Biochem 267:3101-3115.
29. Zhou W, Irwin DC, Escovar-Kousen J, Wilson DB. 2004. Kinetic studies of Thermobifida fusca Cel9A active site mutant enzymes. Biochemistry 43:9655-9663.