Chapter 6 From Live-Cell Imaging to Scanning Electron Microscopy (SEM): The Use of Green Fluorescent Protein (GFP) as a Common Label

Methods in Cell Biology

Volumn 88, Issue , 2008, Pages 97-108

  Drummond, Sheona P ^a   Allen, Terence D ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ANIMAL; BIOLOGICAL MODEL; CELL FUNCTION; CULTURE TECHNIQUE; GENETIC TRANSFECTION; HUMAN; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY; REVIEW; SCANNING ELECTRON MICROSCOPY; STAINING;

ANIMALS; CELL CULTURE TECHNIQUES; CELL PHYSIOLOGY; GREEN FLUORESCENT PROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON, SCANNING; MODELS, BIOLOGICAL; STAINING AND LABELING; TRANSFECTION;

EID: 46249087610     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0091-679X(08)00406-8     Document Type: Review

References (17)

1. Allen T.D., Bennion G.R., Rutherford S.A., Reipert S., Ramalho A., Kiseleva E., and Goldberg M.W. Macromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy. Scanning 19 6 (1997) 403-410
2. Bagley S., Goldberg M.W., Cronshaw J.M., Rutherford S., and Allen T.D. The nuclear pore complex. J. Cell. Sci. 113 (2000) 3885-3886
3. Blegareh N., Rabut G., Bai S.W., van Overbeen M., Beaudouin J., Daigle N., Zatsepina O.V., Pasteau F., Labas V., Fromont-Racine M., Ellenberg J., and Doye V. An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells. J. Cell Biol. 154 6 (2001) 1147-1160
4. Boehmer T., Enninga J., Dales S., Blobel G., and Zhong H. Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc. Natl. Acad. Sci. USA 100 3 (2003) 981-985
5. Drummond S.P., Rutherford S.A., Sanderson H.S., and Allen T.D. High resolution analysis of mammalian nuclear structure throughout the cell cycle: Implications for nuclear pore complex assembly during interphase and mitosis. Can. J. Physiol. Pharmacol. 84 3-4 (2006) 423-430
6. Goldberg M.W., Wiese C., Allen T.D., and Wilson K.L. Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: Evidence for structural intermediates in nuclear pore complex assembly. J. Cell Sci. 110 (1997) 409-420
7. Harel A., Orjalo A.V., Vincent T., Lachish-Zalait A., Vasu S., Shah S., Zimmerman E., Elbaum M., and Forbes D.J. Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores. Mol. Cell. 11 4 (2003) 853-864
8. Joseph J., Tan S.H., Karpova T.S., McNally J., and Dasso M. SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. J. Cell Biol. 156 4 (2002) 595-602
9. Kiseleva E., Allen T.D., Rutherford S., Bucci M., Wente S.R., and Goldberg M.W. Yeast nuclear pore complexes have a cytoplasmic ring and internal filaments. J. Struct. Biol. 145 3 (2004) 272-288
10. Lim R.Y., and Fahrenkrog B. The nuclear pore complex up close. Curr. Opin. Cell Biol. 18 3 (2006) 342-347
11. Loiodice I., Alves A., Rabut G., van Overbeek M., Ellenberg J., Sibarita J.B., and Doye V. The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis. Mol. Biol. Cell. 15 7 (2004) 3333-3344
12. Orjalo A.V., Arnaoutov A., Shen Z., Boyarchuk Y., Zeitlin S.G., Fontoura B., Briggs S., Dasso M., and Forbes D.J. The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol. Biol. Cell. 17 9 (2006) 3806-3818
13. Rasala B.A., Orjalo A.V., Shen Z., Briggs S., and Forbes D.J. ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division. Proc. Natl. Acad. Sci. USA 103 47 (2006) 17801-17806
14. Salina D., Enarson P., Rattner J.B., and Burke B. Nup358 integrates nuclear envelope breakdown with kinetochore assembly. J. Cell Biol. 162 6 (2003) 991-1001
15. Schwartz T.U. Modularity within the architecture of the nuclear pore complex. Curr. Opin. Struct. Biol. 15 2 (2005) 221-226
16. Walther T.C., Alves A., Pickersgill H., Loiodice I., Hetzer M., Galy V., Hulsmann B.B., Kocher T., Wilm M., Allen T., Mattaj I.W., and Doye V. The conserved Nup107-160 complex is critical for nuclear pore complex assembly. Cell 113 2 (2003) 195-206
17. Zuccolo M., Alves A., Galy V., Bolhy S., Formstecher E., Racine V., Sibarita J.B., Fukagawa T., Shiekhattar R., Yen T., and Doye V. The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions. EMBO J. 26 7 (2007) 1853-1864