Challenges in designing a Taqman-based multiplex assay for the simultaneous detection of herpes simplex virus types 1 and 2 and Varicella-zoster virus

Journal of Clinical Virology

Volumn 42, Issue 4, 2008, Pages 326-334

  Weidmann, Manfred ^a   Armbruster, Katrin ^a   Hufert, Frank T ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

ABI 7700; Dye combinations; Herpesvirus; Internal positive control dynamics; Multiplex Taqman PCR

Indexed keywords

CY5 DYE; DYE; FAM DYE; JOE DYE; REPORTER DYE; TXR DYE; VIRUS DNA;

ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; HERPES SIMPLEX; HERPES SIMPLEX VIRUS 1; HERPES SIMPLEX VIRUS 2; INTERNAL POSITIVE CONTROL DYNAMICS; MOLECULAR DYNAMICS; MULTIPLEX POLYMERASE CHAIN REACTION; MULTIPLEX TAQMAN POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SENSITIVITY ANALYSIS; VARICELLA ZOSTER VIRUS; VIRUS DETECTION;

CARBOCYANINES; HERPESVIRUS 1, HUMAN; HERPESVIRUS 2, HUMAN; HERPESVIRUS 3, HUMAN; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; XANTHENES;

EID: 46149086343     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2008.03.005     Document Type: Article

References (40)

1. Aberham C., Pendl C., Gross P., Zerlauth G., and Gessner M. A quantitative, internally controlled real-time PCR Assay for the detection of parvovirus B19 DNA. J Virol Methods 92 2 (2001) 183-191
2. Abu Al-Soud W., and Radstrom P. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J Clin Microbiol 38 12 (2000) 4463-4470
3. Antunes F. Central nervous system AIDS-related diseases. Acta Neurochir 146 10 (2004) 1071-1074
4. Aurelius E., Johansson B., Skoldenberg B., Staland A., and Forsgren M. Rapid diagnosis of herpes-simplex encephalitis by nested polymerase chain-reaction assay of cerebrospinal-fluid. Lancet 337 8735 (1991) 189-192
5. Aurelius E., Johansson B., Skoldenberg B., and Forsgren M. Encephalitis in immunocompetent patients due to herpes-simplex virus Type-1 or Type-2 as determined by type-specific polymerase chain-reaction and antibody-assays of cerebrospinal-fluid. J Med Virol 39 3 (1993) 179-186
6. Bergallo M., Costa C., Margio S., Sidoti F., Terlizzi M.E., and Cavallo R. Development of a multiplex polymerase chain reaction for detection and typing of major human herpesviruses in cerebrospinal fluid. Can J Microbiol 53 10 (2007) 1117-1122
7. Biron K.K. Antiviral drugs for cytomegalovirus diseases. Antiviral Res 71 2-3 (2006) 154-163
8. Corless C.E., Guiver M., Borrow R., Edwards-Jones V., Fox A.J., and Kaczmarski E.B. Simultaneous detection of neisseria meningitidis, haemophilus influenzae, and streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR. J Clin Microbiol 39 4 (2001) 1553-1558
9. Douek D., 2002. 7700 TAQMAN @ listserv.it.northwestern.edu now replaced by qpcrlistserver QPCR listserver.
10. Francois P., Pittet D., Bento M., Pepey B., Vaudaux P., Lew D., et al. Rapid detection of methicillin-resistant staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay. J Clin Microbiol 41 1 (2003) 254-260
11. Grace M.B., McLeland C.B., Gagliardi S.J., Smith J.M., Jackson III W.E., and Blakely W.F. Development and assessment of a quantitative reverse transcription-PCR assay for simultaneous measurement of four amplicons. Clin Chem 49 9 (2003) 1467-1475
12. Gruber F., Falkner F.G., Dorner F., and Hammerle T. Quantitation of viral DNA by real-time PCR applying duplex amplification, internal standardization, and two-color fluorescence detection. Appl Environ Microbiol 67 6 (2001) 2837-2839
13. Hobson-Peters J., O'Loughlin P., and Toye P. Development of an internally controlled, homogeneous polymerase chain reaction assay for the simultaneous detection and discrimination of herpes simplex virus types 1 and 2 and varicella-zoster virus. Mol Cell Probes 21 1 (2007) 24-30
14. Khurana R.N., Charonis A., Samuel M.A., Gupta A., and Tawansy K.A. Intravenous foscarnet in the management of acyclovir-resistant herpes simplex virus type 2 in acute retinal necrosis in children. Med Sci Monit 11 12 (2005) CS75-CS78
15. King D.P., Reid S.M., Hutchings G.H., Grierson S.S., Wilkinson P.J., Dixon L.K., et al. Development of a TaqMan PCR assay with internal amplification control for the detection of African swine fever virus. J Virol Methods 107 1 (2003) 53-61
16. Klein D., Leutenegger C.M., Bahula C., Gold P., Hofmann-Lehmann R., Salmons B., et al. Influence of preassay and sequence variations on viral load determination by a multiplex real-time reverse transcriptase-polymerase chain reaction for feline immunodeficiency virus. J Acquir Immune Defic Syndr 26 1 (2001) 8-20
17. Kreuzer K.A., Bohn A., Lupberger J., Solassol J., le Coutre P., and Schmidt C.A. Simultaneous absolute quantification of target and control templates by real-time fluorescence reverse transcription-PCR using 4-(4′-dimethylaminophenylazo)benzoic acid as a dark quencher dye. Clin Chem 47 3 (2001) 486-490
18. Muir P., and van Loon A.M. Enterovirus infections of the central nervous system. Intervirology 40 2-3 (1997) 153-166
19. Nasarabadi S., Milanovich F., Richards J., and Belgrader P. Simultaneous detection of TaqMan probes containing Fam and Tamra reporter fluorophores. Biotechniques 27 6 (1999) 1116-1118
20. Nazarenko I.A., Bhatnagar S.K., and Hohman R.J. A closed tube format for amplification and detection of DNA based on energy transfer. Nucleic Acids Res 25 12 (1997) 2516-2521
21. Niesters H.G. Quantitation of viral load using real-time amplification techniques. Methods 25 4 (2001) 419-429
22. Niesters H.G. Clinical virology in real time. J Clin Virol 25 Suppl 3 (2002) S3-S12
23. Peter M., Gilbert E., and Delattre O. A multiplex real-time PCR assay for the detection of gene fusions observed in solid tumors. Lab Invest 81 6 (2001) 905-912
24. Schwaiger M., and Cassinotti P. Development of a quantitative real-time RT-PCR assay with internal control for the laboratory detection of tick borne encephalitis virus (TBEV) RNA. J Clin Virol 27 2 (2003) 136-145
25. Sen K., and Asher D.M. Multiplex PCR for detection of enterobacteriaceae in blood. Transfusion 41 11 (2001) 1356-1364
26. Sharma V.K., and Dean-Nystrom E.A. Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins. Vet Microbiol 93 3 (2003) 247-260
27. Templeton K.E., Scheltinga S.A., Sillekens P., Crielaard J.W., van Dam A.P., Goossens H., et al. Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species. J Clin Microbiol 41 9 (2003) 4016-4021
28. Templeton K.E., Scheltinga S.A., van der Zee A., Diederen B.M., van Kruijssen A.M., Goossens H., et al. Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis. J Clin Microbiol 41 9 (2003) 4121-4126
29. Templeton K.E., Scheltinga S.A., Beersma M.F., Kroes A.C., and Claas E.C. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus and parainfluenza viruses 1, 2, 3 and 4. J Clin Microbiol 42 4 (2004) 1564-1569
30. Townsend G.C., and Scheld W.M. Infections of the central nervous system. Adv Internal Med 43 (1998) 403-447
31. Tucker R.A., Unger E.R., Holloway B.P., and Swan D.C. Real-time PCR-based fluorescent assay for quantitation of human papillomavirus types 6, 11, 16 and 18. Mol Diagn 6 1 (2001) 39-47
32. Tyler K. Herpes simplex virus infections of the central nervous system: encephalitis and meningitis, including Mollaret's. Herpes 11 Suppl 2 (2004) 57A-64A
33. Varma-Basil M., El-Hajj H., Marras S.A., Hazbon M.H., Mann J.M., Connell N.D., et al. Molecular beacons for multiplex detection of four bacterial bioterrorism agents. Clin Chem 50 6 (2004) 1060-1062
34. Vet J.A., Majithia A.R., Marras S.A., Tyagi S., Dube S., Poiesz B.J., et al. Multiplex detection of four pathogenic retroviruses using molecular beacons. Proc Natl Acad Sci USA 96 11 (1999) 6394-6399
35. Ward N.L., and Bej A.K. Detection of vibrio parahaemolyticus in shellfish by use of multiplexed real-time PCR with TaqMan fluorescent probes. Appl Environ Microbiol 72 3 (2006) 2031-2042
36. Weidmann M., Meyer-Konig U., and Hufert F.T. Rapid detection of herpes simplex virus and varicella-zoster virus infections by real-time PCR. J Clin Microbiol 41 4 (2003) 1565-1568
37. Welti M., Jaton K., Altwegg M., Sahli R., Wenger A., and Bille J. Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae. Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions. Diagn Microbiol Infect Dis 45 2 (2003) 85-95
38. Westcott D.G., King D.P., Drew T.W., Nowotny N., Kindermann J., Hannant D., et al. Use of an internal standard in a closed one-tube RT-PCR for the detection of equine arteritis virus RNA with fluorescent probes. Vet Res 34 2 (2003) 165-176
39. Whitley R.J., Kimberlin D.W., and Roizman B. Herpes simplex viruses. Clin Infect Dis 26 3 (1998) 541-553 quiz 554-545
40. Widada J., Nojiri H., Kasuga K., Yoshida T., Habe H., and Omori T. Quantification of the carbazole 1,9a-dioxygenase gene by real-time competitive PCR combined with co-extraction of internal standards. FEMS Microbiol Lett 202 1 (2001) 51-57