Simultaneous absolute quantification of target and control templates by real-time fluorescence reverse transcription-PCR using 4-(4′-dimethylaminophenylazo)bezoic acid as a dark quencher dye

Clinical Chemistry

Volumn 47, Issue 3, 2001, Pages 486-490

  Kreuzer, K A ^a   Bohn, A ^a   Lupberger, J ^a   Solassol, J ^a   Le Coutre, P ^a   Schmidt, C A ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

4 (4' DIMETHYLAMINOPHENYLAZO)BENZOIC ACID; BCR ABL PROTEIN; BETA ACTIN; DYE; UNCLASSIFIED DRUG;

ACCURACY; ACUTE GRANULOCYTIC LEUKEMIA; ARTICLE; CHRONIC MYELOID LEUKEMIA; CLINICAL ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; FLUORESCENCE; HUMAN; HUMAN CELL; PHILADELPHIA 1 CHROMOSOME; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0035103619     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/47.3.486     Document Type: Article

References (10)

1. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase
2. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization
3. Correcting for different amplification rates of internal standard and template when measuring cDNA by competitive PCR
4. Internal control for quality assurance of diagnostic RT-PCR
5. Cyanine dyes with high absorption cross section as donor chromophores in energy transfer
6. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
7. Applicability of an absolute quantitative procedure to monitor intra-individual bcr/abl transcript kinetics in clinical samples from chronic myelogenous leukemia patients
8. A highly sensitive and specific fluorescence RT-PCR assay for the pseudogene free detection of β-actin transcripts as quantitative reference
9. Multiplex detection of four pathogenic retroviruses using molecular beacons
10. A new technology for the detection of specific polymerase chain reaction products in a closed tube