FRET evidence that an isoform of caspase-7 binds but does not cleave its substrate

Biochemical and Biophysical Research Communications

Volumn 373, Issue 2, 2008, Pages 325-329

  Li, Isaac T S ^a,b   Pham, Elizabeth ^a  

^a Jui Hsuan)   (Canada)

^b Jui Hsuan)   (Canada)

Author keywords

Biosensor; Caspase 7; Enzyme kinetics; Fluorescence spectroscopy; Fluorescent resonance energy transfer (FRET)

Indexed keywords

CASPASE 7;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; APOPTOSIS; ARTICLE; BIOSENSOR; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDROLYSIS; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION;

ANIMALS; BIOSENSING TECHNIQUES; CASPASE 7; CERCOPITHECUS AETHIOPS; COMPUTER SIMULATION; COS CELLS; FLUORESCENCE RESONANCE ENERGY TRANSFER; ISOENZYMES; KINETICS; SUBSTRATE SPECIFICITY;

EID: 46049090552     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.06.034     Document Type: Article

References (15)

1. Cohen G.M. Caspases: the executioners of apoptosis. Biochem. J. 326 Pt 1 (1997) 1-16
2. Los M., and Walczak H. Caspases: Their Role in Cell Death and Cell Survival, Landes Bioscience (2002), Kluwer Academic/Plenum Pub., Georgetown, TX, New York
3. Shi Y. Mechanisms of caspase activation and inhibition during apoptosis. Mol. Cell 9 (2002) 459-470
4. Yaoita Y. Inhibition of nuclear transport of caspase-7 by its prodomain. Biochem. Biophys. Res. Commun. 291 (2002) 79-84
5. Zhang J., Campbell R.E., Ting A.Y., and Tsien R.Y. Creating new fluorescent probes for cell biology. Na. Rev. Mol. Cell Biol. 3 (2002) 906-918
6. Truong K., and Ikura M. The use of FRET imaging microscopy to detect protein-protein interactions and protein conformational changes in vivo. Curr. Opin. Struct. Biol. 11 (2001) 573-578
7. Chiang J.J., and Truong K. Computational modeling of a new fluorescent biosensor for caspase proteolytic activity improves dynamic range. IEEE Trans. Nanobiosci. 5 (2006) 41-45
8. 2+ signaling. Biotechnol. Lett. 27 (2005) 1219-1227
9. Truong K., Khorchid A., and Ikura M. A fluorescent cassette-based strategy for engineering multiple domain fusion proteins. BMC Biotechnol. 3 (2003) 8
10. Rekas A., Alattia J.R., Nagai T., Miyawaki A., and Ikura M. Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity. J. Biol. Chem. 277 (2002) 50573-50578
11. Hyun Bae J., Rubini M., Jung G., Wiegand G., Seifert M.H., Azim M.K., Kim J.S., Zumbusch A., Holak T.A., Moroder L., Huber R., and Budisa N. Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins. J. Mol. Biol. 328 (2003) 1071-1081
12. Wei Y., Fox T., Chambers S.P., Sintchak J., Coll J.T., Golec J.M., Swenson L., Wilson K.P., and Charifson P.S. The structures of caspases-1, -3, -7 and -8 reveal the basis for substrate and inhibitor selectivity. Chem. Biol. 7 (2000) 423-432
13. Pham E., Chiang J., Li I., Shum W., and Truong K. A computational tool for designing FRET protein biosensors by rigid-body sampling of their conformational space. Structure 15 (2007) 515-523
14. Nagai T., Ibata K., Park E.S., Kubota M., Mikoshiba K., and Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (2002) 87-90
15. Hillel Z., and Wu C.W. Statistical interpretation of fluorescence energy transfer measurements in macromolecular systems. Biochemistry 15 (1976) 2105-2113