A real-time polymerase chain reaction assay to detect single nucleotide polymorphisms at codon 171 in the prion gene for the genotyping of scrapie susceptibility in sheep

Journal of Veterinary Diagnostic Investigation

Volumn 20, Issue 2, 2008, Pages 209-212

  McKay, Jerome Thomas ^a   Brigner, Tiffany A ^a   Caplin, Brian E ^b   McCurdy, Kimberly S ^c   Forde, Richard L ^a  

^a Rocky Mountain Regional Animal Health Laboratory ^*  (United States)

^b Fluoresentric   (United States)

^c MIDWEST RESEARCH INSTITUTE   (United States)

Author keywords

Codon 171; Fluorescence resonance energy transfer (FRET); Fluoresentric; Genotyping; Real time polymerase chain reaction; Scrapie

Indexed keywords

OVIS ARIES;

DNA;

ANIMAL; ANIMAL DISEASE; ARTICLE; CHEMISTRY; GENETIC PREDISPOSITION; GENETICS; GENOTYPE; METHODOLOGY; POLYMERASE CHAIN REACTION; PRION; SCRAPIE; SHEEP; SINGLE NUCLEOTIDE POLYMORPHISM;

ANIMALS; DNA; GENETIC PREDISPOSITION TO DISEASE; GENOTYPE; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; PRIONS; SCRAPIE; SHEEP;

EID: 45849144807     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870802000210     Document Type: Article

References (21)

1. Acutis PL, Sbaiz L, Verburg F, et al.: 2004, Low frequency of the scrapie resistance-associated allele and presence of lysine-171allele of the prion protein gene in Italian Biellese ovine breed. J Gen Virol 85:3165-3172.
2. Alvarez L, Arranz JJ, San Primitivo F: 2006, Identification of a new leucine haplotype (ALQ) at codon 154 in the ovine prion protein gene in Spanish sheep. J Anim Sci 84:259-265.
3. Baylis M, Chihota C, Stevenson E, et al.: 2004, Risk of scrapie in British sheep of different prion protein genotype. J Gen Virol 85:2735-2740.
4. Baylis M, Goldmann W, Houston F, et al.: 2002, Scrapie epidemic in a fully PrP-genotyped sheep flock. J Gen Virol 83:2907-2914.
5. Billinis C, Psychas V, Leontides L, et al.: 2004, Prion protein gene polymorphisms in healthy and scrapie-affected sheep in Greece. J Gen Virol 85:547-554.
6. Bossers A, De Vries R, Smits MA: 2000, Susceptibility of sheep for scrapie as assessed in vitro conversion of nine naturally occurring variants of PrP. J Virol 77:1407-1414.
7. Clouscard C, Beaudry P, Elsen JM, et al.: 1995, Different allelic effects of the codons 136 and 171 of the prion protein gene in sheep with natural scrapie. J Gen Virol 76:2097-2101.
8. Dempsey E, Barton DE, Ryan F: 2004, Detection of five common CFTR mutations by rapid-cycle real-time amplification refractory mutation system PCR. Clin Chem 50:773-775.
9. SC propagation in experimental and natural scrapie in sheep with different PrP genotypes. Vet Pathol 42:258-274.
10. SC for evaluation of scrapie pathogenesis in sheep. J Vet Diagn Invest 19:552-557.
11. Goldmann W, Hunter N, Smith G, et al.: 1994, PrP genotype and agent effects in scrapie: change in allelic interaction with different isolates of agent in sheep, a natural host of scrapie. J Gen Virol 75:989-995.
12. Heaton MP, Leymaster KA, Freking BA, et al.: 2003, Prion gene sequence variation within diverse groups of U.S. sheep, beef, cattle, and deer. Mamm Genome 14:765-777.
13. Johnson ML, Evoniuk JM, Stoltenow CL, et al.: 2007, Development of an assay to determine single nucleotide polymorphisms in the prion gene for the genetic diagnosis of relative susceptibility to classical scrapie in sheep. J Vet Diagn Invest 19:73-77.
14. Kawasaki ES: 1990, DNA isolation from whole blood, PCR-adapted proteinase K method for leucocytes or whole blood. In: PCR: clinical diagnostics and research, ed. A. Rolfs, pp. 84-85. Springer-Verlag, Berlin.
15. Moudjou M, Treguer E, Rezaei H, et al.: 2004, Glycan-controlled epitopes of prion protein include a major determinant of susceptibility to sheep scrapie. J Virol 78:9270-9276.
16. O'Rourke KI, Holyoak GR, Clark WW, et al.: 1997, PrP genotypes and experimental scrapie in orally inoculated Suffolk sheep in the United States. J Gen Virol 78:975-978.
17. Rezaei H, Marc D, Choiset Y, et al.: 2000, High yield purification and physico-chemical properties of full-length recombinant allelic variants of sheep prion protein linked to scrapie susceptibility. Eur J Biochem 267:2833-2839.
18. Schutz E, Scharfenstein M, Brenig B: 2006, Genotyping of ovine prion protein gene (PRNP) variants by PCR with melting curve analysis. Clin Chem 52:1426-1429.
19. Thuring CMA, Erkens JHF, Jacobs JG, et al.: 2004, Discrimination between scrapie and bovine spongiform encephalopathy in sheep by molecular size, immunoreactivity, and glycoprofile of prion protein. J Clin Microbiol 3:972-980.
20. Van Poucke M, Vandesompele J, Mattheeuws, et al.: 2005, A dual fluorescent multiprobe assay for prion protein genotyping in sheep. BMC Infect Dis 5:13.
21. Yuzbasiyan-Gurkan V, Krehbiel JD, Cao Y, Venta PJ: 1999, Development and usefulness of new polymerase chain reaction-based tests for detection of different alleles at codons 136 and 171 of the ovine prion protein gene. Am J Vet Res 60:884-887.