Identification of a Kunitz inhibitor from Albizzia kalkora and its inhibitory effect against pest midgut proteases

Biotechnology Letters

Volumn 30, Issue 8, 2008, Pages 1495-1499

  Zhou, Jia Yu ^a,b   Liao, Hai ^b   Zhang, Nian Hui ^a   Tang, Lin ^a   Xu, Ying ^a   Chen, Fang ^a  

^a SICHUAN UNIVERSITY   (China)

^b SOUTHWEST JIAOTONG UNIVERSITY   (China)

Author keywords

Albizzia kalkora; Isolation; Kunitz inhibitor; Pest control

Indexed keywords

AMMONIUM COMPOUNDS; CHROMATOGRAPHIC ANALYSIS; COMPUTER NETWORKS; EXTRACTION; GEL PERMEATION CHROMATOGRAPHY; MOLECULAR MASS; NETWORK PROTOCOLS; SOLVENT EXTRACTION; SULFATE MINERALS;

(I ,J) CONDITIONS; AMMONIUM SULFATE PRECIPITATION; INHIBITORY EFFECTS; POLYPEPTIDE CHAINS; PURIFICATION PROTOCOLS; SEPHAROSE 4B; TRYPSIN INHIBITORS; WATER EXTRACTION;

MOLECULES;

KUNITZ TYPE PROTEASE INHIBITOR, PLANT; KUNITZ-TYPE PROTEASE INHIBITOR, PLANT; PEPTIDE; PEPTIDE HYDROLASE; PROTEINASE INHIBITOR; VEGETABLE PROTEIN;

ALBIZZIA; ANIMAL; ARTICLE; DIGESTIVE SYSTEM; DRUG EFFECT; ENZYMOLOGY; INSECT; ISOLATION AND PURIFICATION; METABOLISM; PLANT SEED; POLYACRYLAMIDE GEL ELECTROPHORESIS; SPECTROFLUOROMETRY; THERMODYNAMICS; TITRIMETRY;

ALBIZZIA; ANIMALS; DIGESTIVE SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; INSECTS; PEPTIDE HYDROLASES; PEPTIDES; PLANT PROTEINS; PROTEASE INHIBITORS; SEEDS; SPECTROMETRY, FLUORESCENCE; THERMODYNAMICS; TITRIMETRY;

ALBIZIA; HELICOVERPA ARMIGERA; PIERIS RAPAE; SPODOPTERA EXIGUA;

EID: 45849132032     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9699-0     Document Type: Article

References (14)

1. Beekwilder J, Schipper B, Bakker P et al (2000) Characterization of potato proteinase inhibitor II reactive site mutants. Eur J Biochem 267:1975-1984
2. Bhattacharyya A, Mazumdar LS, Babu CR (2007) Bioinsecticidal activity of Archidendron ellipticum trypsin inhibitor on growth and serine digestive enzymes during larval development of Spodoptera litura. Comp Biochem Physiol C 145:669-677
3. Erlanger BE, Kokowsky N, Cohen W (1961) The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys 95:271-278
4. Giri AP, Harsulkar AM, Ku MSB et al (2003) Identification of potent inhibitors of Helicoverpa armigera gut proteinases from winged bean seeds. Phytochemistry 63:523-532
5. Hanspal JS, Bushell GR, Ghosh P (1983) Detection of protease inhibitors using substrate-containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem 132:288-293
6. Harsulkar AM, Giri AP, Patankar AG et al (1999) Successive use of non-host plant proteinase inhibitors required for effective inhibition of gut proteinases and larval growth of Helicoverpa armigera. Plant Physiol 121:497-506
7. Inanaga H, Kobayasi D, Kouzuma Y et al (2001) Protein engineering of novel proteinase inhibitors and their effects on the growth of Spodoptera exigua larvae. Biosci Biotechnol Biochem 65:2259-2264
8. Jongsma MA, Peter J (1995) Adaptation of Spodoptera exigua larva to plant proteinase inhibitors by induction of proteinase activity insensitive of inhibition. Proc Natl Acad Sci USA 92:8041-8045
9. Knights RJ, Light A (1976) Disulfide bond-modified trypsinogen. J Biol Chem 251:222-228
10. Laskowski M Jr, Qasim MA (2000) What can the structures of enzyme-inhibitor complexes tell us about the structures of enzyme substrate complexes? Biochim Biophys Acta 1477:324-337
11. Liao H, Ren W, Kang Z et al (2007) A trypsin inhibitor from Cassia obtusifolia seeds: isolation, characterization and activity on Pieris rapae. Biotechnol Lett 4:653-658
12. Park SY, Lee WYM, Park YK et al (2006) Effects of nitrogen source and bacterial elicitor on isoflavone accumulation in root cultures of Albizzia kalkora (Roxb.) Prain. J Integr Plant Biol 48:1108-1114
13. Ryan CA (1991) Proteinase inhibitors in plants: genes for improving defenses against insects and pathogens. Annu Rev Phytolpathol 28:425-449
14. Terada S, Fujimura S, Katayama H et al (1994) Purification and characterization of two Kunitz family subtilisin inhibitors from seeds of Canavalia lineata. J Biochem 115:392-396