Evaluation of an effective sample prefractionation method for the proteome analysis of breast cancer tissue using narrow range two-dimensional gel electrophoresis

Bioscience, Biotechnology and Biochemistry

Volumn 72, Issue 6, 2008, Pages 1464-1474

  Lee, KiBeom ^a  

^a SongDo Techno Park ^*  (South Korea)

Author keywords

Narrow range two dimensional gel; Prefractionation; Resolution; Solubilization of samples; Two dimensional gel electrophoresis (2 DE)

Indexed keywords

BIOMOLECULES; COLLOIDS; ELECTROPHORESIS; GELATION; MAMMALS; NUCLEAR PROPULSION; OPTIMIZATION; PH; PH EFFECTS; PHOSPHORUS COMPOUNDS; PROPANE; PROTEINS; REDUCING AGENTS; REDUCTION; SOAPS (DETERGENTS); SOLUBILITY; STABILIZERS (AGENTS); TWO DIMENSIONAL; UREA;

(2+1)-DIMENSIONAL; BREAST CANCERS; CARRIER AMPHOLYTES (CA); CHAOTROPES; DITHIOTHREITOL (DTT); HUMAN BREAST CANCER; HUMAN CANCERS; ISOELECTRIC FOCUSING (IEF); OPTIMIZED CONDITIONS; PH RANGE; PREFRACTIONATION; PROTEIN FRACTIONS; PROTEIN PROFILING; PROTEIN SPOTS; PROTEOME ANALYSIS; PROTEOMES; SOLUBILIZED PROTEINS; SUPERIOR RESOLUTION; TWO-DIMENSIONAL GEL ELECTROPHORESIS (2-DGE);

GELS;

BUFFER; PROTEOME; WATER;

ARTICLE; BREAST TUMOR; HUMAN; ISOELECTRIC FOCUSING; METABOLISM; METHODOLOGY; PH; PROTEOMICS; TUMOR CELL LINE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BREAST NEOPLASMS; BUFFERS; CELL LINE, TUMOR; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; PROTEOME; PROTEOMICS; WATER;

MAMMALIA;

EID: 45849117917     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.70777     Document Type: Article

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