Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies

Asian Journal of Andrology

Volumn 10, Issue 4, 2008, Pages 577-584

  Toyama, Yoshiro ^a   Suzuki Toyota, Fumie ^a   Maekawa, Mamiko ^a   Ito, Chizuru ^a   Toshimori, Kiyotaka ^a  

^a CHIBA UNIVERSITY   (Japan)

Author keywords

Actin; Ectoplasmic specialization; Mice; Nectin; Sertolicell; Spermatogenic cell; Testis

Indexed keywords

ACTIN; NECTIN 2; PROTEIN ANTIBODY;

ACTIN FILAMENT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL JUNCTION; CELL MATURATION; CONTROLLED STUDY; ELECTRON MICROSCOPY; MALE; MICROINJECTION; MOUSE; NONHUMAN; PROTEIN EXPRESSION; SEMINIFEROUS TUBULE; SERTOLI CELL; SPERMATID;

ACTINS; ANIMALS; ANTIBODIES; CELL ADHESION MOLECULES; CELL COMMUNICATION; INTERCELLULAR JUNCTIONS; MALE; MICE; MICE, INBRED ICR; MICROFILAMENT PROTEINS; MICROSCOPY, CONFOCAL; SEMINIFEROUS EPITHELIUM; SERTOLI CELLS; SPERMATIDS;

EID: 45749125267     PISSN: 1008682X     EISSN: 17457262     Source Type: Journal    
DOI: 10.1111/j.1745-7262.2008.00357.x     Document Type: Article

References (20)

1. Russell L. Observations on rat Sertoli ectoplasmic ('junctional') specializations in their associations with germ cells of the rat testis. Tissue Cell 1977; 9: 475-98.
2. Yule TD, Montoya GD, Russell LD, Williams TM, Tung KS. Autoantigenic germ cells exist outside the blood testis barrier. J Immunol 1988; 141: 1161-7.
3. Toyama Y. Actin-like filaments in the Sertoli cell junctional specializations in the swine and mouse testis. Anat Rec 1976; 186: 477-92.
4. Cheng CY, Mruk DD. Cell junction dynamics in the testis: Sertoli-germ cell interactions and male contraceptive development. Physiol Rev 2002; 82: 825-74.
5. Yan HH, Mruk DD, Lee WM, Cheng CY. Ectoplasmic specialization: A friend or a foe of spermatogenesis? Bioessays 2007; 29: 36-48.
6. Takai Y, Nakanishi H. Nectin and afadin: Novel organizers of intercellular junctions. J Cell Sci 2003; 116: 17-27.
7. Takahashi K, Nakanishi H, Miyahara M, Mandai K, Satoh K, Satoh A, et al. Nectin/PRR: An immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with afadin, a PDZ domain-containing protein. J Cell Biol 1999; 145: 539-49.
8. Bouchard MJ, Dong Y, McDermott BM Jr, Lam DH, Brown KR, Shelanski M, et al. Defects in nuclear and cytoskeletal morphology and mitochondrial localization in spermatozoa of mice lacking nectin-2, a component of cell-cell adherens junctions. Mol Cell Biol 2000; 20: 2865-73.
9. Ozaki-Kuroda K, Nakanishi H, Ohta H, Tanaka H, Kurihara H, Mueller S, et al. Nectin couples cell-cell adhesion and the actin scaffold at heterotypic testicular junctions. Curr Biol 2002; 12: 1145-50.
10. Mueller S, Rosenquist TA, Takai Y, Bronson RA, Wimmer E. Loss of nectin-2 at Sertoli-spermatid junctions leads to male infertility and correlates with severe spermatozoan head and midpiece malformation, impaired binding to the zona pellucida, and oocyte penetration. Biol Reprod 2003; 69: 1330-40.
11. Inagaki M, Irie K, Ishizaki H, Tanaka-Okamoto M, Miyoshi J, Takai Y. Role of cell adhesion molecule nectin-3 in spermatid development. Gene Cell 2006; 11: 1125-32.
12. Chatot CL, Ziomek CA, Bavister BD, Lewis JL, Torres I. An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J Reprod Fertil 1989; 86: 679-88.
13. Brinster RL, Zimmermann JW. Spermatogenesis following male germ-cell transplantation. Proc Natl Acad Sci U S A 1994; 91: 11298-302.
14. Koh KB, Komiyama M, Toyama Y, Adachi T, Mori C. Percoll fractionation of adult mouse spermatogonia improves germ cell transplantation. Asian J Androl 2004; 6: 93-8.
15. Toyama Y, Maekawa M, Yuasa S. Ectoplasmic specializations in the Sertoli cell: New vistas based on genetic defects and testicular toxicology. Anat Sci Int 2003; 78: 1-16.
16. Suzuki-Toyota F, Ito C, Toyama Y, Maekawa M, Yao R, Noda T, et al. Factors maintaining normal sperm tail structure during epididymal maturation studied in Gopc-/- mice. Biol Reprod 2007; 77: 71-82.
17. Brokelmann J. Fine structure of germ cells and Sertoli cells during the cycle of the seminiferous epithelium in the rat. Z Zellforsch Mikrosk Anat 1963; 59: 820-50.
18. Nicander L. An electron microscopical study of cell contacts in the seminiferous tubules of some mammals. Z Zellforsch Mikrosk Anat 1967; 83: 375-97.
19. Fawcett DW. Ultrastructure and function of the Sertoli cell. In: Hamilton DW, Greep RO, editors. Handbook of Physiology, Section 7: Endocrinology, Vol. 5. Washington DC: American Physiology Society; 1975: 21-55.
20. Toyama Y, Yuasa S. Effects of neonatal administration of 17β-estradiol, β-estradiol 3-benzoate, or bisphenol A on mouse and rat spermatogenesis. Reprod Toxicol 2004; 19: 181-8.