Disruption of metF increased L-lysine production by Methylophilus methylotrophus from methanol

Bioscience, Biotechnology and Biochemistry

Volumn 72, Issue 5, 2008, Pages 1317-1324

  Ishikawa, Kohei ^a,b   Asahara, Takayuki ^a   Gunji, Yoshiya ^a   Yasueda, Hisashi ^a   Asano, Kozo ^b  

^a AJINOMOTO CO INC   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

Author keywords

L lysine production; MetF; Methanol; Methylophilus methylotrophus

Indexed keywords

AMINES; CARBON; ENCODING (SYMBOLS); HEALTH; METHANOL; ORGANIC ACIDS;

AUXOTROPHIC MUTANTS; CORYNEBACTERIUM GLUTAMICUM (PROPCG); DIHYDRODIPICOLINATE SYNTHASE; FEEDBACK INHIBITION; L-LYSINE (PLL); L-LYSINE PRODUCTION; METHIONINE (M); OPEN READING FRAME (ORF); REDUCTASE (BVR-A); SOLE CARBON SOURCE;

AMINO ACIDS;

5,10 METHYLENETETRAHYDROFOLATE REDUCTASE (FADH2); HOMOSERINE KINASE; LYSINE; METHANOL; METHIONINE; PHOSPHOTRANSFERASE;

ARTICLE; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; METABOLISM; METHYLOPHILUS METHYLOTROPHUS; MUTATION;

5,10-METHYLENETETRAHYDROFOLATE REDUCTASE (FADH2); LYSINE; METHANOL; METHIONINE; METHYLOPHILUS METHYLOTROPHUS; MUTATION; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR);

CORYNEBACTERIUM GLUTAMICUM; METHYLOPHILUS METHYLOTROPHUS; TETRA;

EID: 45749110512     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.70818     Document Type: Article

References (26)

1. Jenkins, O., Byrom, D., and Jones, D., Methylophilus: a new genusof methanol-utilizing bacteria. Int. J. Syst. Bacteriol., 37, 446-448 (1987).
2. Senior, P. J., and Windass, J., The ICI single cell protein process. Biotechnol. Lett., 2, 205-210 (1980).
3. Anthony, C., The commercial exploitation of methylotrophs. In "The Biochemistry of the Methylotrophus," ed. Anthony, C., Academic Press, London, pp. 328-378 (1982).
4. Windass, J. D., Worsey, M. J., Pioli, E. M., Barth, P. T., Atherton, K. T., Dart, E. C., Byrom, D., Powell, K., and Senior, P. J., Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus. Nature, 287, 396-401 (1980).
5. Pfefferle, W., Mockel, B., bathe, B., and Marx, A., Biotechnological manufacture of lysine. Adv. Biochem. Eng. Biotechnol., 79, 59-112 (2003).
6. Motoyama, H., Yano, H., Terasaki, Y., and Anazawa, H., Overproduction of L-Lysine from methanol by Methylobacillus glycogens derivatives carrying a plasmid with a mutated dapA gene. Appl. Environ. Microbiol., 67, 3064-3070 (2001).
7. Lee, G. H., Hur, W., Bremmon, C. C., and Flickinger, M. C., Lysine production from methanol at 50°C using Bacillus methanolicus: modeling and productivity using a three-phase continuous simulation. Biotechnol. Bioeng., 49, 639-653 (1996).
8. Gunji, Y., Tsujimoto, N., Shimaoka, M., Ogawa-Miyata, Y., Sugimoto, S., and Yasueda, H., Characterization of the L-lysine biosynthetic pathway in an obligate methylotroph, Methylophilus methylotrophus. Biosci. Biotechnol. Biochem., 68, 1449-1460 (2004).
9. Tsujimoto, N., Gunji, Y., Ogawa-Miyata, Y., Shimaoka, S., and Yasueda, H., L-Lysine biosynthetic pathway of Methylophilus methylotrophus and construction of an L-lysine producer. J. Biotechnol., 124, 327-337 (2006).
10. Gunji, Y., and Yasueda, H., Enhancement of L-lysine production in methylotroph Methylophilus methylotrophus by introducing a mutant LysE exporter. J. Biotechnol., 127, 1-13 (2006).
11. Tosaka, O., Hirakawa, H., Takinami, K., and Hirose, Y., Regulation of lysine biosynthesis by leucine in Brevibacterium lactofermentum. Agric. Biol. Chem., 42, 1501-1506 (1978).
12. Petek, M., Krumbach, K., Eggeling, L., and Sahm, H., Leucine synthesis in Corynebacterium glutamicum: enzyme activities, structure of leuA, and effect of leuA inactivation on lysine biosynthesis. Appl. Environ. Microbiol., 60, 133-140 (1994).
13. Fernández-González, C., Gil, J. A., Mateos, L. M., Schwarzer, A., Schäfer, A., Kalinowski, J., Pühler, A., and Martín, J. F., Construction of L-lysine-overproducing strains of Brevibacterium lactofermentum by targeted disruption of the hom and thrB genes. Appl. Microbiol. Biotechnol., 46, 554-558 (1996).
14. Silman, N. J., Carver, M. A., and Jones, C. W., Directed evolution of amidase in Methylophilus methylotrophus: purification and properties of amidases from wild-type and mutant strains. J. Gen. Microbiol., 137, 169-178 (1991).
15. Sambrook, J., and Russell, D., "Molecular Cloning, a Laboratory Manual" 3rd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor (2001).
16. Gliesche, C. G., Transformation of methylotrophic bacteria by electroporation. Can. J. Microbiol., 43, 197-201 (1996).
17. These, J., Kleidman, L., and Girons, I. S., Homoserine kinase from Escherichia coli K-12: properties, inhibition by L-threonine, and regulation of biosynthesis. J. Bacteriol., 118, 577-581 (1974).
18. Kosuge, T., Gao, D., and Hoshino, T., Analysis of the methionine biosynthetic pathway in the extremely thermophilic eubacterium Thermus thermophilus. J. Biosci. Biotechnol., 90, 271-279 (2000).
19. Greene, R. C., Biosynthesis of methionine. In "Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology" 2nd ed., eds. Neidhardt, F., and others, American Society for Microbiology, Washington DC, pp. 542-560 (1996).
20. Burr, B., Walker, J., Truffa-bachi, P., and Cohen, G. N., Homoserine kinase from Escherichia coli K12. Eur. J. Biochem., 62, 519-526 (1976).
21. Byung-Joon, H., Hye-Jin, Y., Younhee, K., and Heung-shick, L., Corynebacterium glutamicum utilizes both transsulfuration and directsulfhydrilation pathways for methionine biosynthesis. J. Bacteriol., 184, 1277-1286 (2002).
22. Kikuchi, Y., Kojima, H., and Tanaka, T., Mutational analysis of the feedback sites of lysine-sensitive aspartokinase of Escherichia coli. FEMS Microbiol. Lett., 173, 211-215 (1999).
23. Kojima, H., Ogawa, M., Kawamura, K., and Sano, K., Process for producing L-lysine. W/O 95/16042 (1995).
24. Akashi, K., Shibai, H., and Hirose, H., The effect of aeration and agitation on amino acid fermentation. Hakko to Kogyo (in Japanese), 37, 321 (1979).
25. Shimizu, E., Oosumi, T., Heima, H., Tanaka, T., Kurashige, J., Enei, H., Miwa, K., and Nakamori, S., Culture conditions for improvement of L-threonine production using a genetically self-cloned L-threonine hyperproducing strain of Escherichia coli K-12. Biosci. Biotechnol. Biochem., 59, 1095-1098 (1995).
26. Ishida, M., Sato, K., Hashiguchi, K., Ito, H., Enei, H., and Nakamori, S., High fermentative production of L-threonine from acetate by a Brevibacterium flavum stabilized strain transformed with a recombinant plasmid carrying the Escherichia coli thr operon. Biosci. Biotechnol. Biochem., 57, 1755-1756 (1993).