Mutational analysis of the feedback sites of lysine-sensitive aspartokinase of Escherichia coli

FEMS Microbiology Letters

Volumn 173, Issue 1, 1999, Pages 211-215

  Kikuchi, Yoshimi ^a   Kojima, Hiroyuki ^a   Tanaka, Takashi ^a  

^a AJINOMOTO CO INC   (Japan)

Author keywords

Aspartokinase III; Escherichia coli; lysC gene

Indexed keywords

ASPARTATE KINASE; LYSINE; METHIONINE; THREONINE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; MISSENSE MUTATION; MOLECULAR CLONING; MUTANT; NEGATIVE FEEDBACK; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ASPARTATE KINASE; DNA MUTATIONAL ANALYSIS; ESCHERICHIA COLI; FEEDBACK; GENE EXPRESSION REGULATION, BACTERIAL; HYDROXYLAMINE; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PLASMIDS;

EID: 0033039869     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00075-0     Document Type: Article

References (15)

1. Patte, J.C. (1996) Biosynthesis of threonine and lysine. In: Escherichia coli and Salmonella typhimurium (Neidhardt, F.C., Ed.), Vol. 2, pp. 528-541. American Society for Microbiology, Washington, DC.
2. Theze, J., Margarita, D., Cohen, G.N., Borne, F. and Patte, J.C. (1974) Mapping of the structural genes of the three aspartokinases and of the two homoserine dehydrogenases of Escherichia coli K-12. J. Bacteriol. 117, 133-143.
3. Katinka, M., Cossart, P., Sibilli, L., Saint-Girons, I., Chalvignac, M.A., Le Bras, G., Cohen, G.N. and Yaniv, M. (1980) Nucleotide sequence of the thrA gene of Escherichia coli. Proc. Natl. Acad. Sci. USA 77, 5730-5733.
4. Zakin, M.M., Duchange, N., Ferrara, P. and Cohen, G.N. (1983) Nucleotide sequence of the metL gene of Escherichia coli. J. Biol. Chem. 258, 3028-3031.
5. Cassan, M., Parsot, C., Cohen, G.N. and Patte, J.C. (1986) Nucleotide sequence of lysC encoding the lysine-sensitive aspartokinase III of Escherichia coli K-12. J. Biol. Chem. 261, 1052-1057.
6. Falco, S.C., Guida, T., Locke, M., Mauvais, J., Sanders, C. and Webber, P. (1995) Transgenic canola and soybean with increased lysine. BioTechnology 13, 577-582.
7. Kalinowski, J., Cremer, J., Bachmann, B., Eggeling, L., Sahm, H. and Puhler, A. (1991) Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. Mol. Microbiol. 5, 1197-1204.
8. Omori, K., Imai, Y., Suzuki, S. and Komatsubara, S. (1993) Nucleotide sequence of the Serratia marcescens threonine operon and analysis of the threonine operon mutations which alter feedback inhibition of both aspartate kinase I and homoserine dehydrogenase I. J. Bacteriol. 175, 785-794.
9. Omori, K. and Komatsubara, S. (1993) Role of serine 352 in the allosteric response of Serratia marcescens aspartate kinase I-homoserine dehydrogenase I analyzed by using site-directed mutagenesis. J. Bacteriol. 175, 959-965.
10. Berlyn, M.K.B., Low, K.B. and Rudd, K.E. (1996) Linkage map of Escherichia coli K-12, edition 9. In: Escherichia coli and Salmonella typhimurium (Neidhardt, F.C., Ed.), Vol. 2, pp. 1715-1902. American Society for Microbiology, Washington, DC.
11. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
12. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
13. Kikuchi, Y., Tsujimoto, K. and Kurahashi, O. (1997) Mutational analysis of the feedback sites of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli. Appl. Environ. Microbiol. 63, 761-762.
14. Stadtman, E.R., Cohen, G.N., Le Bras, G. and de Robichon-Szulmajster, H. (1961) Feed-back inhibition and repression of aspartokinase activity in Escherichia coli and Saccharomyces cerevisiae. J. Biol. Chem. 236, 2033-2038.
15. Omori, K., Suzuki, S., Imai, Y. and Komatsubara, S. (1991) Analysis of the Serratia marcescens proBA operon and feedback control of proline biosynthesis. J. Gen. Microbiol. 137, 509-517.