Diagnosis of Trichophyton rubrum from onychomycotic nail samples using polymerase chain reaction and calcofluor white microscopy

Journal of the American Podiatric Medical Association

Volumn 98, Issue 3, 2008, Pages 224-228

  Gupta, Aditya K ^a,b   Zaman, Muhammad ^b   Singh, Jagpal ^b  

^a UNIVERSITY OF TORONTO   (Canada)

^b MEDIPROBE RESEARCH INC   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 45549102194     PISSN: 87507315     EISSN: None     Source Type: Journal    
DOI: 10.7547/0980224     Document Type: Review

References (35)

1. CAMPBELL AW, ANYANWU EC, MORAD M: Evaluation of the drug treatment and persistence of onychomycosis. Scientific World J 4: 760, 2004.
2. GUPTA AK, JAIN HC, LYNDE CW, ET AL: Prevalence and epidemiology of onychomycosis in patients visiting physicians' offices: a multicenter Canadian survey of 15,000 patients. J Am Acad Dermatol 43: 244, 2000.
3. ROBERTS DT: Prevalence of dermatophyte onychomycosis in the United Kingdom: results of an omnibus survey. Br J Dermatol 126 (suppl 39): 23, 1992.
4. SUMMERBELL RC, KANE J, KRAJDEN S: Onychomycosis, tinea pedis and tinea manuum caused by non-dermatophytic filamentous fungi. Mycoses 32: 609, 1989.
5. GUPTA AK, KONNIKOV N, MACDONALD P, ET AL: Prevalence and epidemiology of toenail onychomycosis in diabetic subjects: a multicentre survey. Br J Dermatol 139: 665, 1998.
6. GUPTA AK, SCHER RK, DE DONCKER P: Current management of onychomycosis: an overview. Dermatol Clin 15: 121, 1997.
7. EVANS EG: Causative pathogens in onychomycosis and the possibility of treatment resistance: a review. J Am Acad Dermatol 38: S32, 1998.
8. GRASER Y, KUIJPERS AF, PRESBER W, ET AL: Molecular taxonomy of Trichophyton mentagrophytes and T. torisurans. Med Mycol 37: 315, 1999.
9. GUPTA AK, RYDER JE, SKINNER AR: Treatment of onychomycosis: pros and cons of antifungal agents. J Cutan Med Surg 8: 25, 2004.
10. SCHER RK, BARAN R: Onychomycosis in clinical practice: factors contributing to recurrence. Br J Dermatol 149 (suppl 65): 5, 2003.
11. LECHA M, EFFENDY I, FEUILHADE DE CHAUVIN M, ET AL: Treatment options: development of consensus guidelines. J Eur Acad Dermatol Venereol 19 (suppl 1): 25, 2005.
12. OLAFSSON JH, SIGURGEIRSSON B, BARAN R: Combination therapy for onychomycosis. Br J Dermatol 149 (suppl 65): 15, 2003.
13. EVANS EG, SIGURGEIRSSON B: Double blind, randomised study of continuous terbinafine compared with intermittent itraconazole in treatment of toenail onychomycosis. BMJ 318: 1031, 1999.
14. GUPTA AK, DE DONCKER P, SCHER RK, ET AL: Itraconazole for the treatment of onychomycosis. Int J Dermatol 37: 303, 1998.
15. EPSTEIN E: How often does oral treatment of toenail onychomycosis produce a disease-free nail? an analysis of published data. Arch Dermatol 134: 1551, 1998.
16. MONOD M, BAUDRAZ-ROSSELET F, RAMELET AA, ET AL: Direct mycological examination in dermatology: a comparison of different methods. Dermatologica 179: 183, 1989.
17. ZABAWSKI EJ JR, STYLES AR, COCKERELL CJ: Routine periodic acid-Schiff staining of nail plate fragments in fungal cultures for onychomycosis: a method to increase the sensitivity of diagnosis. Cutis 66: 456, 2000.
18. WEINBERG JM, KOESTENBLATT EK, JENNINGS MB: Utility of histopathologic analysis in the evaluation of onychomycosis. JAPMA 95: 258, 2005.
19. ANANE S, AOUN K, ZALLAGUA N, ET AL: Onychomycosis in Tunis area: epidemiological and mycological data [in French]. Ann Dermatol Venereol 128: 733, 2001.
20. BAUDRAZ-ROSSELET F, FRENK E: In vitro nail invasion by pathogenic and non-pathogenic fungi under different culture conditions. Mycoses 33: 553, 1990.
21. LATEUR N, MORTAKI A, ANDRE J: Two hundred ninety-six cases of onychomycosis in children and teenagers: a 10-year laboratory survey. Pediatr Dermatol 20: 385, 2003.
22. SUMMERBELL RC, COOPER E, BUNN U, ET AL: Onychomycosis: a critical study of techniques and criteria for confirming the etiologic significance of nondermatophytes. Med Mycol 43: 39, 2005.
23. GUPTA AK, RYDER JE, BARAN R, ET AL: Non-dermatophyte onychomycosis. Dermatol Clin 21: 257, 2003.
24. GUPTA AK, ZAMAN M, SINGH J: Fast and sensitive detection of Trichopyton rubrum DNA from the nail samples of patients with onychomycosis by a double-round polymerase chain reaction-based assay. Br J Dermtol 157: 698, 2007.
25. ANDERSON TD, ROSS JP, ROBY RK, ET AL: A validation study for the extraction and analysis of DNA from human nail material and its application to forensic casework. J Forensic Sci 44: 1053, 1999.
26. AVNER S, NIR N, HENRI T: Combination of oral terbinafine and topical ciclopirox compared to oral terbinafine for the treatment of onychomycosis. J Dermatol Treat 16: 327, 2005.
27. GUPTA AK: Ciclopirox topical solution, 8% combined with oral terbinafine to treat onychomycosis: a randomized, evaluator-blinded study. J Drugs Dermatol 4: 481, 2005.
28. GUPTA AK, LYNCH LE: Management of onychomycosis: examining the role of monotherapy and dual, triple, or quadruple therapies. Cutis 74: 5, 2004.
29. CLAYTON YM: Clinical and mycological diagnostic aspects of onychomycoses and dermatomycoses. Clin Exp Dermatol 17 (suppl 1): 37, 1992.
30. FLETCHER CL, HAY RJ, SMEETON NC: Onychomycosis: the development of a clinical diagnostic aid for toenail disease: part I. Establishing discriminating historical and clinical features. Br J Dermatol 150: 701, 2004.
31. MIDGLEY G, MOORE MK, COOK JC, ET AL: Mycology of nail disorders. J Am Acad Dermatol 31: S68, 1994.
32. WEINBERG JM, KOESTENBLATT EK, TUTRONE WD, ET AL: Comparison of diagnostic methods in the evaluation of onychomycosis. J Am Acad Dermatol 49: 193, 2003.
33. ARCA E, SARACLI MA, AKAR A, ET AL: Polymerase chain reaction in the diagnosis of onychomycosis. Eur J Dermatol 14: 52, 2004.
34. KARDJEVA V, SUMMERBELL R, KANTARDJIEV T, ET AL: Forty-eight-hour diagnosis of onychomycosis with subtyping of Trichophyton rubrum, strains. J Clin Microbiol 44: 1419, 2006.
35. OKEKE CN, TSUBOI R, KAWAI M, ET AL: Isolation of an intron-containing partial sequence of the gene encoding dermatophyte actin (ACT) and detection of a fragment of the transcript by reverse transcription-nested PCR as a means of assessing the viability of dermatophytes in skin scales. J Clin Microbiol 39: 101, 2001.